RSL1D1

RSL1D1
Identifikatori
Aliasi	RSL1D1
Vanjski ID-jevi	OMIM: 615874 MGI: 1913659 HomoloGene: 41052 GeneCards: RSL1D1
Lokacija gena (čovjek)
Hrom.	Hromosom 16 (čovjek)
Kraj	11,851,580 bp
Lokacija gena (miš)
Hrom.	Hromosom 16 (miš)
Kraj	11,021,195 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• cadherin binding; • vezivanje sa RNK; • mRNA 3'-UTR binding; • mRNA 5'-UTR binding;
Ćelijska komponenta	• Jedarce; • membrana; • jedro; • cytosolic large ribosomal subunit; • 90S preribosome;
Biološki proces	• regulation of apoptotic process; • regulation of cellular senescence; • osteoblast differentiation; • regulation of protein localization; • Biosinteza bjelančevina; • maturation of LSU-rRNA;
	Izvori:Amigo / QuickGO
Ortolozi
	26156
	66409
	ENSG00000171490
	ENSMUSG00000005846
	O76021
	Q8BVY0
	NM_015659
	NM_025546
	NP_056474
	NP_079822
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Ribosomni L1 domen-sadržavajući protein 1 je protein koji je kod ljudi kodiran genom RSL1D1.^[5]^[6]^[7]

Ma et al. (2008) izvijestili su da se gen RSL1D1 nalazi na kratkom (p) kraku hromosoma 16, pozicija 16p13.^[8]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 490 aminokiselina, a molekulska težina 54.973 Da.^[9]

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin

H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MEDSASASLS	SAAATGTSTS	TPAAPTARKQ	LDKEQVRKAV	DALLTHCKSR
KNNYGLLLNE	NESLFLMVVL	WKIPSKELRV	RLTLPHSIRS	DSEDICLFTK
DEPNSTPEKT	EQFYRKLLNK	HGIKTVSQII	SLQTLKKEYK	SYEAKLRLLS
SFDFFLTDAR	IRRLLPSLIG	RHFYQRKKVP	VSVNLLSKNL	SREINDCIGG
TVLNISKSGS	CSAIRIGHVG	MQIEHIIENI	VAVTKGLSEK	LPEKWESVKL
LFVKTEKSAA	LPIFSSFVSN	WDEATKRSLL	NKKKKEARRK	RRERNFEKQK
ERKKKRQQAR	KTASVLSKDD	VAPESGDTTV	KKPESKKEQT	PEHGKKKRGR
GKAQVKATNE	SEDEIPQLVP	IGKKTPANEK	VEIQKHATGK	KSPAKSPNPS
TPRGKKRKAL	PASETPKAAE	SETPGKSPEK	KPKIKEEAVK	EKSPSLGKKD
ARQTPKKPEA	KFFTTPSKSV	RKASHTPKKW	PKKPKVPQST

Kloniranje i ekspresija

Supresivnom subtraktivnom hibridizacijom za identifikaciju proteina smanjenih s replikativnom senescencijom u 2BS ljudskim diploidnim fibroblastima, nakon čega slijedi PCR amplifikacija, Ma et al. (2008) klonirali su RSL1D1, koji su nazvali CSIG. Izvedeni protein s 490 aminokiselina ima N-terminalni ribosomski L1 (MRPL1) domen i C-terminalni domen bogat lizinom. Northern blot analiza otkrila je varijabilnu ekspresiju CSIG u većini ispitivanih ljudskih tkiva, s najvećom ekspresijom u srcu, skeletnim mišićima i posteljici, a malo ili nimalo u mozgu, debelom crijevu i timusu. Endogeni CSIG lokaliziran je pretežno na jedarcetu u 2BS i ćelijama HeLa, frakcioniran polimerima ćelija HEK293. Western blot analizom otkriven je CSIG pri prividnoj molekulskoj masi od 55 kD.

Funkcija gena

UPA (PLAU) je proteaza vanćelijskog matriksa koja je uključena u migraciju ćelija. Korištenjem 1-hibridnog mjesta kvasca i EMSA-e, Tong et al. (2005) otkrili su da je RSL1D1, ili PBK1, povezao pojačivač u promotoru UPA, između dva AP1-lokusa. Eksperimenti prekomjerne ekspresije i nokdauna sa ćelijskim linijama ljudskog karcinoma pluća 95D potvrdili su da je PBK1 pojačavao ekspresiju UPA, vezivanjem za pojačivač.^[10] Ma et al. (2008) otkrili su da prekomjerna ekspresija CSIG u 2BS fibroblastima pospješuje rast ćelija, povećava broj ćelika u S fazi i odgađa starenje. Suprotno tome, utišavanje CSIG-a pomoću male ometajuće RNK usporilo je rast ćelija, smanjilo broj ćelija u S i G2 /M fazama i ubrzalo ćelijsko starenje. Analize gena reportera i Western blot analiza otkrile su da su CSIG negativno regulirali PTEN i njegov efektor p27 (KIP1) (CDKN1B), a oba su potrebna za replikativno starenje. Studije vezivanja pokazale su da je CSIG stupio u interakciju sa određenim segmentom PTEN-ovog 5-UTR-a, moguće indirektno i smanjio regulaciju PTEN-translacije, što je rezultiralo destabilizacijom p27 (KIP1). Ekspresija PTEN-a bila je presudna za CSIG-ovisnu ekspresiju p27 (KIP1) i napredovanje ćelijskog ciklusa.

Struktura gena

Ma et al. (2008) utvrdili su da gen RSL1D1 sadrži devet egzona i obuhvata 5,1 kb.

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000171490 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000005846 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P (Oct 2004). "Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer". Oncogene. 23 (46): 7734–45. doi:10.1038/sj.onc.1207921. PMID 15334068.
^ Huch G, Hohn HP, Denker HW (Mar 1999). "Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR". Placenta. 19 (8): 557–67. doi:10.1016/S0143-4004(98)90015-7. PMID 9859858.
^ "Entrez Gene: RSL1D1 ribosomal L1 domain containing 1".
^ Ma, L., Chang, N., Guo, S., Li, Q., Zhang, Z., Wang, W., Tong, T. CSIG inhibits PTEN translation in replicative senescence. Molec. Cell. Biol. 28: 6290-6301, 2008. PubMed: 18678645
^ "UniProt, O76021". Pristupljeno 9. 7. 2021.
^ Tong, C., Tan, L., Li, P., Zhu, Y.-S. Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells. Acta Biochim. Biophys. Sinica 37: 303-309, 2005.

Dopunska literatura

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Tong C, Tan L, Li P, Zhu YS (2005). "Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells". Acta Biochim. Biophys. Sin. (Shanghai). 37 (5): 303–9. doi:10.1111/j.1745-7270.2005.00041.x. PMID 15880258.
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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000171490 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000005846 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid15334068-5] Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P (Oct 2004). "Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer". Oncogene. 23 (46): 7734–45. doi:10.1038/sj.onc.1207921. PMID 15334068.

[pmid9859858-6] Huch G, Hohn HP, Denker HW (Mar 1999). "Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR". Placenta. 19 (8): 557–67. doi:10.1016/S0143-4004(98)90015-7. PMID 9859858.

[entrez-7] "Entrez Gene: RSL1D1 ribosomal L1 domain containing 1".

[8] Ma, L., Chang, N., Guo, S., Li, Q., Zhang, Z., Wang, W., Tong, T. CSIG inhibits PTEN translation in replicative senescence. Molec. Cell. Biol. 28: 6290-6301, 2008. PubMed: 18678645

[UniProt-9] "UniProt, O76021". Pristupljeno 9. 7. 2021.

[10] Tong, C., Tan, L., Li, P., Zhu, Y.-S. Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells. Acta Biochim. Biophys. Sinica 37: 303-309, 2005.

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