UBL3

UBL3
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2GOW
Identifikatori
Aliasi	UBL3
Vanjski ID-jevi	OMIM: 604711 MGI: 1344373 HomoloGene: 5153 GeneCards: UBL3
Lokacija gena (čovjek)
Hrom.	Hromosom 13 (čovjek)
Kraj	29,850,617 bp
Lokacija gena (miš)
Hrom.	Hromosom 5 (miš)
Kraj	148,552,789 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ortolozi
	5412
	24109
	ENSG00000122042
	ENSMUSG00000001687
	O95164
	Q9Z2M6
	NM_007106
	NM_011908
	NP_009037
	NP_036038; NP_001346128; NP_001346129; NP_001346130; NP_001346131
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Ubikvitinoliki protein 3 je protein je kod ljudi kodiran genom UBL3.^[5]^[6]

Hibridnim mapiranjem somatskih ćelija, Chadwick et al. (1999) locirali su ljidskii gen UBL3 na hromosomu 13, sekvenca 13q12-q13. Koristeći fluorescentnu hibridizaciju in situ, mapirali su i mišji gen Ubl3 na telomerni kraj hromosoma 5 u pojasu G2-3, regiji koja pokazuje homologiju sintenije sa ljudskim 13q.^[7]

U melanocitnim ćelijama ekspresija gena UBL3 može se regulirati transkripcijskim faktorom, povezanim s mikroftalmijom (MITF).^[8]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 227 aminokiselina, a molekulska težina 13.157 Da.^[9]

10	20	30	40	50
MSSNVPADMI	NLRLILVSGK	TKEFLFSPND	SASDIAKHVY	DNWPMDWEEE
QVSSPNILRL	IYQGRFLHGN	VTLGALKLPF	GKTTVMHLVA	RETLPEPNSQ
GQRNREKTGE	SNCCVIL

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Trodimenzijska struktura ubikvitinskog proteina

Ubikvitin cilja proteine za razgradnju 26S, proteasomom. Nasuprot tome, proteini slični ubikvitinu (UBL) (npr. UBL1) možda nisu direktno uključeni u razgradnju proteina, ali izgleda da imaju raznolik raspon ćelijskih funkcija (pregled Hodges et al., 1998). Gorepomenuti Chadwick et al. (1999) identifikovali su gen Drosophila UBL3. Pretražujući EST bazu podataka, koristeći cDNK Drosophila UBL3 kao upit, identifikovali su ljudski UBL3 EST. Koristeći EST, izolirali su cDNK matičnih ćelija ljudskih keratinocita koje imaju kompletnu sekvencu kodiranja UBL3. Izvedeni ljudski protein UBL3sa 117 aminokiselina sadrži dva potencijalna mjesta N-glikozilacije, potencijalno mjesto fosforilacije C-proteinske kinaze i potencijalno mjesto prednilacije C-terminala. Relativno je hidrofilan, bez predviđenih transmembranskih domena. Ljudski UBL3 deli 69% identiteta aminokiselinske sekvence sa jednim oblikom drozofilinog UBL3 i 99% identiteta sa mišjim Ubl3, cDNK. Northern blot analiza ljudskih tkiva otkrila je transkripte UBL3 od 3,5 i 4,5 kb u svim testiranim tkivima, kao što su slezena, timus, leukociti periferne krvi, sjemenici, jajnici, posteljica, prostata, jetra, [[gušterača, tanko i debelo crijevo, te bubreg, srce, pluća, mozak i skeletni mišići. Dodatni transkripti od 2,0 i 3,0 kb pronađeni su u testisima. Identificirali su tri signala poliadenilacije, koji rezultiraju alternativno poliadeniliranim transkriptima UBL3.^[10]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000122042 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000001687 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Chadwick BP, Kidd T, Sgouros J, Ish-Horowicz D, Frischauf AM (Aug 1999). "Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene". Gene. 233 (1–2): 189–95. doi:10.1016/S0378-1119(99)00138-9. PMID 10375635.
^ "Entrez Gene: UBL3 ubiquitin-like 3".
^ Chadwick, B. P., Kidd, T., Sgouros, J., Ish-Horowicz, D., Frischauf, A.-M. Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene. Gene 233: 189-195, 1999. PubMed: 10375635
^ Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971.
^ "UniProt, O95164". Pristupljeno 11. 9. 2017.
^ Hodges, M., Tissot, C., Freemont, P. S. Protein regulation: tag wrestling with relatives of ubiquitin. Curr. Biol. 8: R749-R752, 1998. PubMed: 9799726

Dopunska literatura

Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Downes BP, Saracco SA, Lee SS, et al. (2006). "MUBs, a family of ubiquitin-fold proteins that are plasma membrane-anchored by prenylation". J. Biol. Chem. 281 (37): 27145–57. doi:10.1074/jbc.M602283200. PMID 16831869.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000122042 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000001687 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10375635-5] Chadwick BP, Kidd T, Sgouros J, Ish-Horowicz D, Frischauf AM (Aug 1999). "Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene". Gene. 233 (1–2): 189–95. doi:10.1016/S0378-1119(99)00138-9. PMID 10375635.

[entrez-6] "Entrez Gene: UBL3 ubiquitin-like 3".

[7] Chadwick, B. P., Kidd, T., Sgouros, J., Ish-Horowicz, D., Frischauf, A.-M. Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene. Gene 233: 189-195, 1999. PubMed: 10375635

[pmid19067971-8] Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971.

[UniProt-9] "UniProt, O95164". Pristupljeno 11. 9. 2017.

[10] Hodges, M., Tissot, C., Freemont, P. S. Protein regulation: tag wrestling with relatives of ubiquitin. Curr. Biol. 8: R749-R752, 1998. PubMed: 9799726

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