WNT7B

WNT7B
Identifikatori
Aliasi	WNT7B
Vanjski ID-jevi	OMIM: 601967 MGI: 98962 HomoloGene: 22531 GeneCards: WNT7B
Lokacija gena (čovjek)
Hrom.	Hromosom 22 (čovjek)
Kraj	45,977,162 bp
Lokacija gena (miš)
Hrom.	Hromosom 15 (miš)
Kraj	85,466,674 bp
Ontologija gena
Molekularna funkcija	• GO:0005110 frizzled binding; • signaling receptor binding; • receptor ligand activity;
Ćelijska komponenta	• endocytic vesicle membrane; • endoplasmic reticulum lumen; • extracellular region; • Golgi lumen; • Egzosom; • ćelijska membrana; • citoplazma; • Vanćelijsko;
Biološki proces	• cellular response to retinoic acid; • cell fate commitment; • renal inner medulla development; • lung morphogenesis; • mammary gland epithelium development; • stem cell proliferation; • embryonic organ development; • lung development; • renal outer medulla development; • embryonic placenta morphogenesis; • synapse organization; • metanephric epithelium development; • in utero embryonic development; • trachea cartilage morphogenesis; • chorio-allantoic fusion; • multicellular organism development; • developmental growth involved in morphogenesis; • forebrain regionalization; • positive regulation of osteoblast differentiation; • cell metabolism; • metanephric loop of Henle development; • neuron differentiation; • central nervous system vasculogenesis; • fibroblast proliferation; • lobar bronchus development; • metanephros morphogenesis; • inner medullary collecting duct development; • metanephric collecting duct development; • establishment or maintenance of polarity of embryonic epithelium; • lung epithelium development; • outer medullary collecting duct development; • oxygen homeostasis; • lens fiber cell development; • homeostatic process; • regulation of cell projection size; • response to glucocorticoid; • neuron projection morphogenesis; • Wnt-signalni put; • neuron projection development; • positive regulation of JNK cascade; • canonical Wnt signaling pathway; • regulation of signaling receptor activity;
	Izvori:Amigo / QuickGO
Ortolozi
	7477
	22422
	ENSG00000188064
	ENSMUSG00000022382
	P56706
	P28047
	NM_058238
	NM_001163633; NM_001163634; NM_009528
	NP_478679
	NP_001157105; NP_001157106; NP_033554
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Protein Wnt-7b jest protein koji je kod ljudi kodiran genom WNT7B sa hromosoma 22. Wnt7b je signalni protein koji ima ključnu ulogu u mnogim razvojnim procesima, uključujući formiranje posteljice, pluća, očiju, dendrita i kostiju zajedno s razvojem bubrega. Primarna uloga Wnt7b je uspostavljanje kortikojezgarne ose organizacije epitela.^[5]^[6]^[7]^[8]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 349 aminokiselina, a molekulska težina 39.327 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MHRNFRKWIF	YVFLCFGVLY	VKLGALSSVV	ALGANIICNK	IPGLAPRQRA
ICQSRPDAII	VIGEGAQMGI	NECQYQFRFG	RWNCSALGEK	TVFGQELRVG
SREAAFTYAI	TAAGVAHAVT	AACSQGNLSN	CGCDREKQGY	YNQAEGWKWG
GCSADVRYGI	DFSRRFVDAR	EIKKNARRLM	NLHNNEAGRK	VLEDRMQLEC
KCHGVSGSCT	TKTCWTTLPK	FREVGHLLKE	KYNAAVQVEV	VRASRLRQPT
FLRIKQLRSY	QKPMETDLVY	IEKSPNYCEE	DAATGSVGTQ	GRLCNRTSPG
ADGCDTMCCG	RGYNTHQYTK	VWQCNCKFHW	CCFVKCNTCS	ERTEVFTCK

Funkcija

Porodica gena WNT sastoji se od strukturno povezanih gena koji kodiraju izlučene signalizirajuće proteine. Ovi proteini uključeni su u onkogenezu i u nekoliko razvojnih procesa, kao što je regulacija sudbine ćelije usnjeravanje tokom embriogeneze. Ovaj gen je član porodice gena WNT. Kodira protein koji pokazuje 99% aminokiselinskog identiteta miša i i 91% Wnt7A proteina u rodu Xenopus. Među članovima ljudske porodice WNT, ovaj protein je najsličniji proteinu WNT7A (77,1% ukupnog aminokiselinskog identiteta). Ovaj gen može igrati važnu ulogu u razvoju i napredovanju raka želuca, jednjaka i gušterače.^[8]

Uloga u bubrezima

Wnt7b je ključni parakrini signalni faktor kojeg luči epitel mokraćovoda, a uspostavlja kortiko-sržnu osovinu sisarskog bubrega.^[9] Wnt7b je signalni protein koji ima ključnu ulogu za mnoge razvojne procese, uključujući formiranje placente, pluća, oka, dendrita i kostiju zajedno s razvojem bubrega. Primarna uloga Wnt7b je uspostavljanje kortiko-sržne osovine organizacije epitela. Uspostavljanje kortiko-medulske ose igra bitnu ulogu za razvoj komponente bubrežne moždine. Wnt7b regulira orijentaciju ćelijskih dioba u epitelu sabirnog kanala bubrežne moždine, u kojem je glavna struktura koja pokreće formiranje bubrežne srži. Postoje dva intersticijska regulatora koji su eksplicitno povezani sa razvojem medule (srži, moždine); Pod1 koji kodira transkripcijski faktor eksprimiran u bubrežnom intersticiju, dok p57Kip2 kodira inhibitor kinaze, zavisne od ciklina koji je povezan sa Beckwith-Wiedemannovim sindromom. Pod1, p57Kip2 i integrin α3 (ITGA3) su tri faktora koja su uključena u morfogenezu bubrežne srži. Nokaut Pod1 rezultira izostankom formiranja bubrežne medule, dok nokauti p57Kip2 i Itga3 rezultiraju smanjenom bubrežnom moždinom. Uklanjanje aktivnosti Wnt7b dovodi do neuspjeha u razvoju medule, dok ostali aspekti razvoja bubrega, uključujući grananje mokraćovoda, razvoj kore bubrega i nefrogenezu, ostaju nepromijenjeni. Odsustvo bubrežne medule također utiče na ravan diobe epitelnih ćelija zajedno sa malim proliferativnim rastom Henleove petlje. Wnt7b nulti alel će rezultirati smrtnim ishodom zbog smanjenja funkcije placente, što dovodi do neuspjeha u pokretanju organogeneze.^[9]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000188064 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022382 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL (May 1994). "Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue". Cancer Research. 54 (10): 2615–2621. PMID 8168088.
^ van Bokhoven H, Kissing J, Schepens M, van Beersum S, Simons A, Riegman P, McMahon JA, McMahon AP, Brunner HG (Sep 1997). "Assignment of WNT7B to human chromosome band 22q13 by in situ hybridization". Cytogenetics and Cell Genetics. 77 (3–4): 288–289. doi:10.1159/000134600. hdl:2066/25317. PMID 9284940.
^ Kirikoshi H, Sekihara H, Katoh M (October 2001). "Molecular cloning and characterization of human WNT7B". International Journal of Oncology. 19 (4): 779–783. doi:10.3892/ijo.19.4.779. PMID 11562755.
^ ^a ^b ^c "Entrez Gene: WNT7B wingless-type MMTV integration site family, member 7B".
^ ^a ^b Yu J, Carroll TJ, Rajagopal J, Kobayashi A, Ren Q, McMahon AP (January 2009). "A Wnt7b-dependent pathway regulates the orientation of epithelial cell division and establishes the cortico-medullary axis of the mammalian kidney". Development. 136 (1): 161–171. doi:10.1242/dev.022087. PMC 2685965. PMID 19060336.

Dopunska literatura

Smolich BD, McMahon JA, McMahon AP, Papkoff J (December 1993). "Wnt family proteins are secreted and associated with the cell surface". Molecular Biology of the Cell. 4 (12): 1267–1275. doi:10.1091/mbc.4.12.1267. PMC 275763. PMID 8167409.
Tanaka K, Okabayashi K, Asashima M, Perrimon N, Kadowaki T (July 2000). "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family". European Journal of Biochemistry. 267 (13): 4300–4311. doi:10.1046/j.1432-1033.2000.01478.x. PMID 10866835.
Capurro MI, Shi W, Sandal S, Filmus J (December 2005). "Processing by convertases is not required for glypican-3-induced stimulation of hepatocellular carcinoma growth". The Journal of Biological Chemistry. 280 (50): 41201–41206. doi:10.1074/jbc.M507004200. PMID 16227623.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000188064 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022382 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8168088-5] Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL (May 1994). "Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue". Cancer Research. 54 (10): 2615–2621. PMID 8168088.

[pmid9284940-6] van Bokhoven H, Kissing J, Schepens M, van Beersum S, Simons A, Riegman P, McMahon JA, McMahon AP, Brunner HG (Sep 1997). "Assignment of WNT7B to human chromosome band 22q13 by in situ hybridization". Cytogenetics and Cell Genetics. 77 (3–4): 288–289. doi:10.1159/000134600. hdl:2066/25317. PMID 9284940.

[pmid11562755-7] Kirikoshi H, Sekihara H, Katoh M (October 2001). "Molecular cloning and characterization of human WNT7B". International Journal of Oncology. 19 (4): 779–783. doi:10.3892/ijo.19.4.779. PMID 11562755.

[entrez-8] "Entrez Gene: WNT7B wingless-type MMTV integration site family, member 7B".

[Yu_2009-9] Yu J, Carroll TJ, Rajagopal J, Kobayashi A, Ren Q, McMahon AP (January 2009). "A Wnt7b-dependent pathway regulates the orientation of epithelial cell division and establishes the cortico-medullary axis of the mammalian kidney". Development. 136 (1): 161–171. doi:10.1242/dev.022087. PMC 2685965. PMID 19060336.

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