PGDB5

Dostupne proteinske strukture:
Pfam	strukture / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	sažetak strukture

PiggyBac izvedeni prenosivi element 5/Transposaza IS4
PiggyBac izvedeni prenosivi element 5/Transposaza IS4
Identifikatori
Simbol	DDE_Tnp_1_7, PGBD
Pfam	PF13843
InterPro	IPR029526
Pfam
Dostupne proteinske strukture:
Pfam	strukture / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	sažetak strukture

PGDB5
Identifikatori
Aliasi
Vanjski ID-jevi	GeneCards: [1]
Ortolozi
	n/a
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	n; a
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	Wikipodaci
Pogledaj/uredi – čovjek

PiggyBac izvedeni prenosivi element 5 jest enzim koji je kod ljudi kodiran genom PGBD5 sa hromosoma 1.^[1] PGBD5 is a DNA transposase related to the ancient PiggyBac transposaza prci puta identificurana je kod kupudnog moljca, Trichoplusia ni.^[2] Vjeruje se da je gen pripitomljen prije više od 500 miliona godina kod zajedničkog pretka glavonožaca i kičmenjaka.^[3]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 455 aminokiselina, a molekulska težina 51.642Da.^[4]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MPDYRTRALF	VQSTLGDSAG	PELQLLSIVP	GRDLQPSDSF	TGPTRKMPPS
ASAVDFFQLF	VPDNVLKNMV	VQTNMYAKKF	QERFGSDGAW	VEVTLTEMKA
FLGYMISTSI	SHCESVLSIW	SGGFYSNRSL	ALVMSQARFE	KILKYFHVVA
FRSSQTTHGL	YKVQPFLDSL	QNSFDSAFRP	SQTQVLHEPL	IDEDPVFIAT
CTERELRKRK	KRKFSLWVRQ	CSSTGFIIQI	YVHLKEGGGP	DGLDALKNKP
QLHSMVARSL	CRNAAGKNYI	IFTGPSITSL	TLFEEFEKQG	IYCCGLLRAR
KSDCTGLPLS	MLTNPATPPA	RGQYQIKMKG	NMSLICWYNK	GHFRFLTNAY
SPVQQGVIIK	RKSGEIPCPL	AVEAFAAHLS	YICRYDDKYS	KYFISHKPNK
TWQQVFWFAI	SIAINNAYIL	YKMSDAYHVK	RYSRAQFGER	LVRELLGLED
ASPTH

Funkcija

Pretpostavljena katalitska trijada proteina sastavljenog od tri asparaginskokiselinska ostatka, tokom evolucije, konzervirana je među genima sličnim PGBD5,^[5] a razlikuje se od drugih gena sličnih PiggyBac-u.^[3] PGBD5 has been shown to be able to transpose DNA in a sequence-specific, cut-and-paste fashion.^[5] Također je predloženo da PGBD5 posreduje u preuređenju DNK, specifičnih za mjesto u ljudskim tumorima.^[6]

Ljudski PGBD5 može mobilizirati transpozone insekata PiggyBac u kulturi ljudskih ćelija.^[7]

Ekspresija u mozgu

U moždanom tkivu zrelih miševa, PGBD5 se nalazi prvenstveno u regijama olfaktornog bulbusa, hipokampusu i cerebelumu. U moždanom tkiva mišjih embriona, PGBD5 se nalazi ne samo u medijalnom paliju i pretponsnom istmusu, koji su područja mozga koja se razvojijaju u hipokampus i mali mozak već i u područjima u embrionskom dijelu mozga koji stvara hipotalamus i medulu u korteksnom tkivu.

Klinički začaj

PGBD5 je eksprimiran u većini dječijih solidnih tumora.^[8] Ekspresija gena pojačana je kod sporadične Creutzfeldt-Jakobove bolesti.^[9] PGBD5 je povezan s frontotemporalnom demencijom, gdje je najizraženiji u neuronima, zatim oliodendrocitima, zrelim astrocitima, fetusnim astrocitima, endotelnim ćelijama i potom u mikroglijama/makrofagima.^[10]

Reference

^ "PGBD5 piggyBac transposable element derived 5 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Pristupljeno 8. 4. 2017.
^ Toman J (april 1979). "A course to pursue". Nursing Times. 75 (17): 694–5. doi:10.1128/JVI.47.2.287-300.1983. PMC 255260. PMID 16789244.
^ ^a ^b Pavelitz T, Gray LT, Padilla SL, Bailey AD, Weiner AM (novembar 2013). "PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans". Mobile DNA. 4 (1): 23. doi:10.1186/1759-8753-4-23. PMC 3902484. PMID 24180413.
^ "UniProt, Q8N414" (jezik: engleski). Pristupljeno 16. 12. 2021.
^ ^a ^b Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A (septembar 2015). "Genomic DNA transposition induced by human PGBD5". eLife. 4. doi:10.7554/eLife.10565. PMC 4625184. PMID 26406119.
^ Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still E, MacArthur IC, Rodríguez-Fos E, Gonzalez S, Puiggròs M, Blackford AN, Mason CE, de Stanchina E, Gönen M, Emde AK, Shah M, Arora K, Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CW, Steen H, Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A (juli 2017). "PGBD5 promotes site-specific oncogenic mutations in human tumors". Nature Genetics. 49 (7): 1005–1014. doi:10.1038/ng.3866. PMC 5489359. PMID 28504702.
^ Ivics, Zoltán (maj 2016). "Endogenous Transposase Source in Human Cells Mobilizes piggyBac Transposons". Molecular Therapy (jezik: engleski). 24 (5): 851–854. doi:10.1038/mt.2016.76. PMC 4881781. PMID 27198853.
^ Research, American Association for Cancer (1. 1. 2018). "The DNA Transposase PGBD5 Sensitizes Tumors to Inhibition of DNA Repair". Cancer Discovery (jezik: engleski). 8 (1): OF17–OF17. doi:10.1158/2159-8290.CD-RW2017-213. ISSN 2159-8274. PMID 29127084.
^ Vastrad, Basavaraj; Vastrad, Chanabasayya; Kotturshetti, Iranna (24. 12. 2020). "Identification of potential key genes and pathway linked with sporadic Creutzfeldt-Jakob disease based on integrated bioinformatics analyses". medRxiv (jezik: engleski): 2020.12.21.20248688. doi:10.1101/2020.12.21.20248688.
^ Broce, Iris; Karch, Celeste M.; Wen, Natalie; Fan, Chun C.; Wang, Yunpeng; Tan, Chin Hong; Kouri, Naomi; Ross, Owen A.; Höglinger, Günter U.; Muller, Ulrich; Hardy, John (9. 1. 2018). "Immune-related genetic enrichment in frontotemporal dementia: An analysis of genome-wide association studies". PLOS Medicine (jezik: engleski). 15 (1): e1002487. doi:10.1371/journal.pmed.1002487. ISSN 1549-1676. PMC 5760014. PMID 29315334.

Vanjski linkovi

[1] "PGBD5 piggyBac transposable element derived 5 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Pristupljeno 8. 4. 2017.

[2] Toman J (april 1979). "A course to pursue". Nursing Times. 75 (17): 694–5. doi:10.1128/JVI.47.2.287-300.1983. PMC 255260. PMID 16789244.

[Pavelitz_et_al.-3] Pavelitz T, Gray LT, Padilla SL, Bailey AD, Weiner AM (novembar 2013). "PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans". Mobile DNA. 4 (1): 23. doi:10.1186/1759-8753-4-23. PMC 3902484. PMID 24180413.

[UniProt-4] "UniProt, Q8N414" (jezik: engleski). Pristupljeno 16. 12. 2021.

[:0-5] Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A (septembar 2015). "Genomic DNA transposition induced by human PGBD5". eLife. 4. doi:10.7554/eLife.10565. PMC 4625184. PMID 26406119.

[6] Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still E, MacArthur IC, Rodríguez-Fos E, Gonzalez S, Puiggròs M, Blackford AN, Mason CE, de Stanchina E, Gönen M, Emde AK, Shah M, Arora K, Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CW, Steen H, Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A (juli 2017). "PGBD5 promotes site-specific oncogenic mutations in human tumors". Nature Genetics. 49 (7): 1005–1014. doi:10.1038/ng.3866. PMC 5489359. PMID 28504702.

[7] Ivics, Zoltán (maj 2016). "Endogenous Transposase Source in Human Cells Mobilizes piggyBac Transposons". Molecular Therapy (jezik: engleski). 24 (5): 851–854. doi:10.1038/mt.2016.76. PMC 4881781. PMID 27198853.

[8] Research, American Association for Cancer (1. 1. 2018). "The DNA Transposase PGBD5 Sensitizes Tumors to Inhibition of DNA Repair". Cancer Discovery (jezik: engleski). 8 (1): OF17–OF17. doi:10.1158/2159-8290.CD-RW2017-213. ISSN 2159-8274. PMID 29127084.

[9] Vastrad, Basavaraj; Vastrad, Chanabasayya; Kotturshetti, Iranna (24. 12. 2020). "Identification of potential key genes and pathway linked with sporadic Creutzfeldt-Jakob disease based on integrated bioinformatics analyses". medRxiv (jezik: engleski): 2020.12.21.20248688. doi:10.1101/2020.12.21.20248688.

[10] Broce, Iris; Karch, Celeste M.; Wen, Natalie; Fan, Chun C.; Wang, Yunpeng; Tan, Chin Hong; Kouri, Naomi; Ross, Owen A.; Höglinger, Günter U.; Muller, Ulrich; Hardy, John (9. 1. 2018). "Immune-related genetic enrichment in frontotemporal dementia: An analysis of genome-wide association studies". PLOS Medicine (jezik: engleski). 15 (1): e1002487. doi:10.1371/journal.pmed.1002487. ISSN 1549-1676. PMC 5760014. PMID 29315334.

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