MGAT1
Alfa-1,3-manozil-glikoprotein 2-beta-N-acetilglukozaminiltransferaza jest enzim koji je kod ljudi kodiran genom MGAT1 sa hromosoma 5.[5][6]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 445 aminokiselina, а molekulska težina 50.878 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MLKKQSAGLV | LWGAILFVAW | NALLLLFFWT | RPAPGRPPSV | SALDGDPASL | ||||
| TREVIRLAQD | AEVELERQRG | LLQQIGDALS | SQRGRVPTAA | PPAQPRVPVT | ||||
| PAPAVIPILV | IACDRSTVRR | CLDKLLHYRP | SAELFPIIVS | QDCGHEETAQ | ||||
| AIASYGSAVT | HIRQPDLSSI | AVPPDHRKFQ | GYYKIARHYR | WALGQVFRQF | ||||
| RFPAAVVVED | DLEVAPDFFE | YFRATYPLLK | ADPSLWCVSA | WNDNGKEQMV | ||||
| DASRPELLYR | TDFFPGLGWL | LLAELWAELE | PKWPKAFWDD | WMRRPEQRQG | ||||
| RACIRPEISR | TMTFGRKGVS | HGQFFDQHLK | FIKLNQQFVH | FTQLDLSYLQ | ||||
| REAYDRDFLA | RVYGAPQLQV | EKVRTNDRKE | LGEVRVQYTG | RDSFKAFAKA | ||||
| LGVMDDLKSG | VPRAGYRGIV | TFQFRGRRVH | LAPPLTWEGY | DPSWN |
Funkcija uredi
Postoji više od 100 različitih glikoziltransferaza uključenih u sintezu i vezanje proteina vezanih i za lipidne oligosaharide. UDP-N-acetilglukozamin: alfa-3-D-manozid beta-1,2-N-acetilglukozaminiltransferaza I je posredujući enzim Golgijevog aparata, bitan za sintezu hibridnih i složenih N-glikana. Protein, kodiran jednim egzonom, pokazuje tipska obilježja transmembranskog proteina tipa II. Vjeruje se da je protein bitan za normalnu embriogenezu.[6]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000131446 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000020346 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Hull E, Sarkar M, Spruijt MP, Hoppener JW, Dunn R, Schachter H (Jun 1991). "Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene". Biochem Biophys Res Commun. 176 (2): 608–15. doi:10.1016/S0006-291X(05)80227-X. PMID 1827260.
- ^ a b "Entrez Gene: MGAT1 mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase".
- ^ "UniProt, P26572" (jezik: engleski). Pristupljeno 17. 10. 2021.
Dopunska literatura uredi
- Kumar R, Yang J, Eddy RL, et al. (1992). "Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I". Glycobiology. 2 (4): 383–93. doi:10.1093/glycob/2.4.383. PMID 1421759.
- Kumar R, Yang J, Larsen RD, Stanley P (1991). "Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation". Proc. Natl. Acad. Sci. U.S.A. 87 (24): 9948–52. doi:10.1073/pnas.87.24.9948. PMC 55291. PMID 1702225.
- Tan J, D'Agostaro AF, Bendiak B, et al. (1995). "The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein". Eur. J. Biochem. 231 (2): 317–28. doi:10.1111/j.1432-1033.1995.tb20703.x. PMID 7635144.
- Yip B, Chen SH, Mulder H, et al. (1997). "Organization of the human beta-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesis". Biochem. J. 321 (2): 465–74. doi:10.1042/bj3210465. PMC 1218092. PMID 9020882.
- Yen CL, Stone SJ, Cases S, et al. (2002). "Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase". Proc. Natl. Acad. Sci. U.S.A. 99 (13): 8512–7. Bibcode:2002PNAS...99.8512Y. doi:10.1073/pnas.132274899. PMC 124292. PMID 12077311.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
- Saribas AS, Johnson K, Liu L, et al. (2007). "Refolding of human beta-1-2 GlcNAc transferase (GnT1) and the role of its unpaired Cys 121". Biochem. Biophys. Res. Commun. 362 (2): 381–6. doi:10.1016/j.bbrc.2007.07.199. PMID 17716624.