HYOU1

HYOU1
Identifikatori
Aliasi	HYOU1
Vanjski ID-jevi	OMIM: 601746 MGI: 108030 HomoloGene: 4658 GeneCards: HYOU1
Lokacija gena (čovjek)
Hrom.	Hromosom 11 (čovjek)
Kraj	119,057,227 bp
Lokacija gena (miš)
Hrom.	Hromosom 9 (miš)
Kraj	44,303,666 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• nucleotide binding; • chaperone binding; • ATP binding; • GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• endocytic vesicle lumen; • endoplasmic reticulum lumen; • membrana; • focal adhesion; • smooth endoplasmic reticulum; • endoplasmic reticulum chaperone complex; • extracellular region; • Endoplazmatski retikulum; • Egzosom;
Biološki proces	• negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway; • negative regulation of apoptotic process; • receptor-mediated endocytosis; • response to endoplasmic reticulum stress; • endoplasmic reticulum to Golgi vesicle-mediated transport; • IRE1-mediated unfolded protein response; • response to ischemia; • cellular response to hypoxia; • negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway;
	Izvori:Amigo / QuickGO
Ortolozi
	10525
	12282
	ENSG00000280682; ENSG00000149428
	ENSMUSG00000032115
	Q9Y4L1; Q9BST8
	Q9JKR6
	NM_001130991; NM_006389
	NM_021395
	NP_001124463; NP_006380
	NP_067370
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Hipoksijski nadregulirani protein 1 je protein koji je kod ljudi kodiran genom HYOU1.^[5]^[6]^[7]

Protein kodiran ovim genom pripada porodici proteina toplotnog šoka 70. Ovaj gen ima tri iRNK zbog upotrebe alternativnih mjesta za transkripciju. Cis-djelujući segment nalazi se na 5' kraju egzona 1A, koji je uključen u indukciju ovisnu o stresu. Transkript koji počinje s egzonom 1B prvenstveno je induciran hipoksijom, što rezultira nakupljanjem ovog proteina u endoplazmatskom retikulumu (ER).

Smatra se da protein kodiran ovim genom igra važnu ulogu u savijanju proteina i lučenju u ER. Budući da je potiskivanje proteina povezano s ubrzanom apoptozom, također se sugerira da ima važnu citoprotektivnu ulogu u ćelijskim poremećajima uzrokovanim hipoksijom. Pokazalo se da je ovaj protein nadreguliran u tumorima, posebno u tumorima dojke, pa je povezan s invazivnošću tumora.

Postoji i alternativno mjesto za translaciju ovog gena kojem nedostaje signalni peptid. Ovaj protein bez signalnog peptida je samo tri aminokiseline kraći od zrelog proteina u ER-u, a smatra se da ima funkciju održavanja u citosolu.^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 999 aminokiselina, a molekulska težina 111.335 Da.^[8].

10	20	30	40	50
MADKVRRQRP	RRRVCWALVA	VLLADLLALS	DTLAVMSVDL	GSESMKVAIV
KPGVPMEIVL	NKESRRKTPV	IVTLKENERF	FGDSAASMAI	KNPKATLRYF
QHLLGKQADN	PHVALYQARF	PEHELTFDPQ	RQTVHFQISS	QLQFSPEEVL
GMVLNYSRSL	AEDFAEQPIK	DAVITVPVFF	NQAERRAVLQ	AARMAGLKVL
QLINDNTATA	LSYGVFRRKD	INTTAQNIMF	YDMGSGSTVC	TIVTYQMVKT
KEAGMQPQLQ	IRGVGFDRTL	GGLEMELRLR	ERLAGLFNEQ	RKGQRAKDVR
ENPRAMAKLL	REANRLKTVL	SANADHMAQI	EGLMDDVDFK	AKVTRVEFEE
LCADLFERVP	GPVQQALQSA	EMSLDEIEQV	ILVGGATRVP	RVQEVLLKAV
GKEELGKNIN	ADEAAAMGAV	YQAAALSKAF	KVKPFVVRDA	VVYPILVEFT
REVEEEPGIH	SLKHNKRVLF	SRMGPYPQRK	VITFNRYSHD	FNFHINYGDL
GFLGPEDLRV	FGSQNLTTVK	LKGVGDSFKK	YPDYESKGIK	AHFNLDESGV
LSLDRVESVF	ETLVEDSAEE	ESTLTKLGNT	ISSLFGGGTT	PDAKENGTDT
VQEEEESPAE	GSKDEPGEQV	ELKEEAEAPV	EDGSQPPPPE	PKGDATPEGE
KATEKENGDK	SEAQKPSEKA	EAGPEGVAPA	PEGEKKQKPA	RKRRMVEEIG
VELVVLDLPD	LPEDKLAQSV	QKLQDLTLRD	LEKQEREKAA	NSLEAFIFET
QDKLYQPEYQ	EVSTEEQREE	ISGKLSAAST	WLEDEGVGAT	TVMLKEKLAE
LRKLCQGLFF	RVEERKKWPE	RLSALDNLLN	HSSMFLKGAR	LIPEMDQIFT
EVEMTTLEKV	INETWAWKNA	TLAEQAKLPA	TEKPVLLSKD	IEAKMMALDR
EVQYLLNKAK	FTKPRPRPKD	KNGTRAEPPL	NASASDQGEK	VIPPAGQTED
AEPISEPEKV	ETGSEPGDTE	PLELGGPGAE	PEQKEQSTGQ	KRPLKNDEL

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

Reference

^ ^a ^b ^c ENSG00000149428 GRCh38: Ensembl release 89: ENSG00000280682, ENSG00000149428 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032115 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Ikeda J, Kaneda S, Kuwabara K, Ogawa S, Kobayashi T, Matsumoto M, Yura T, Yanagi H (Feb 1997). "Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150". Biochem Biophys Res Commun. 230 (1): 94–9. doi:10.1006/bbrc.1996.5890. PMID 9020069.
^ Ozawa K, Kuwabara K, Tamatani M, Takatsuji K, Tsukamoto Y, Kaneda S, Yanagi H, Stern DM, Eguchi Y, Tsujimoto Y, Ogawa S, Tohyama M (Mar 1999). "150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death". J Biol Chem. 274 (10): 6397–404. doi:10.1074/jbc.274.10.6397. PMID 10037731.
^ ^a ^b "Entrez Gene: HYOU1 hypoxia up-regulated 1".
^ "UniProt, Q9Y4L1". Pristupljeno 23. 8. 2021.

Dopunska literatura

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Tsukamoto Y, Kuwabara K, Hirota S, et al. (1996). "150-kD oxygen-regulated protein is expressed in human atherosclerotic plaques and allows mononuclear phagocytes to withstand cellular stress on exposure to hypoxia and modified low density lipoprotein". J. Clin. Invest. 98 (8): 1930–41. doi:10.1172/JCI118994. PMC 507633. PMID 8878445.
Kuznetsov G, Chen LB, Nigam SK (1997). "Multiple molecular chaperones complex with misfolded large oligomeric glycoproteins in the endoplasmic reticulum". J. Biol. Chem. 272 (5): 3057–63. doi:10.1074/jbc.272.5.3057. PMID 9006956.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Tsukamoto Y, Kuwabara K, Hirota S, et al. (1998). "Expression of the 150-kd oxygen-regulated protein in human breast cancer". Lab. Invest. 78 (6): 699–706. PMID 9645760.
Bando Y, Ogawa S, Yamauchi A, et al. (2000). "150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells". Am. J. Physiol., Cell Physiol. 278 (6): C1172–82. doi:10.1152/ajpcell.2000.278.6.C1172. PMID 10837345.
Kaneda S, Yura T, Yanagi H (2000). "Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions". J. Biochem. 128 (3): 529–38. doi:10.1093/oxfordjournals.jbchem.a022783. PMID 10965054.
Tamatani M, Matsuyama T, Yamaguchi A, et al. (2001). "ORP150 protects against hypoxia/ischemia-induced neuronal death". Nat. Med. 7 (3): 317–23. doi:10.1038/85463. PMID 11231630. S2CID 10152200.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Zhang H, Li XJ, Martin DB, Aebersold R (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry". Nat. Biotechnol. 21 (6): 660–6. doi:10.1038/nbt827. PMID 12754519. S2CID 581283.
Bando Y, Tsukamoto Y, Katayama T, et al. (2005). "ORP150/HSP12A protects renal tubular epithelium from ischemia-induced cell death". FASEB J. 18 (12): 1401–3. doi:10.1096/fj.03-1161fje. PMID 15240565. S2CID 30637967.
Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry". J. Proteome Res. 4 (3): 931–40. doi:10.1021/pr050024a. PMID 15952740.
Lewandrowski U, Moebius J, Walter U, Sickmann A (2006). "Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach". Mol. Cell. Proteomics. 5 (2): 226–33. doi:10.1074/mcp.M500324-MCP200. PMID 16263699.
Liu T, Qian WJ, Gritsenko MA, et al. (2006). "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry". J. Proteome Res. 4 (6): 2070–80. doi:10.1021/pr0502065. PMC 1850943. PMID 16335952.
Cechowska-Pasko M, Bankowski E, Chene P (2006). "The effect of hypoxia on the expression of 150 kDa oxygen-regulated protein (ORP 150) in HeLa cells". Cell. Physiol. Biochem. 17 (1–2): 89–96. doi:10.1159/000091467. PMID 16543725.
Takeuchi S (2007). "Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone". Protein J. 25 (7–8): 517–28. doi:10.1007/s10930-006-9038-z. PMID 17131193. S2CID 41452314.
Kitao Y, Matsuyama T, Takano K, et al. (2007). "Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?". Antioxid. Redox Signal. 9 (5): 589–95. doi:10.1089/ars.2006.1518. PMID 17330988.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

[refGRCh38Ensembl-1] ENSG00000149428 GRCh38: Ensembl release 89: ENSG00000280682, ENSG00000149428 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000032115 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9020069-5] Ikeda J, Kaneda S, Kuwabara K, Ogawa S, Kobayashi T, Matsumoto M, Yura T, Yanagi H (Feb 1997). "Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150". Biochem Biophys Res Commun. 230 (1): 94–9. doi:10.1006/bbrc.1996.5890. PMID 9020069.

[pmid10037731-6] Ozawa K, Kuwabara K, Tamatani M, Takatsuji K, Tsukamoto Y, Kaneda S, Yanagi H, Stern DM, Eguchi Y, Tsujimoto Y, Ogawa S, Tohyama M (Mar 1999). "150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death". J Biol Chem. 274 (10): 6397–404. doi:10.1074/jbc.274.10.6397. PMID 10037731.

[entrez-7] "Entrez Gene: HYOU1 hypoxia up-regulated 1".

[UniProt-8] "UniProt, Q9Y4L1". Pristupljeno 23. 8. 2021.

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