HYOU1
Hipoksijski nadregulirani protein 1 je protein koji je kod ljudi kodiran genom HYOU1.[5][6][7]
Protein kodiran ovim genom pripada porodici proteina toplotnog šoka 70. Ovaj gen ima tri iRNK zbog upotrebe alternativnih mjesta za transkripciju. Cis-djelujući segment nalazi se na 5' kraju egzona 1A, koji je uključen u indukciju ovisnu o stresu. Transkript koji počinje s egzonom 1B prvenstveno je induciran hipoksijom, što rezultira nakupljanjem ovog proteina u endoplazmatskom retikulumu (ER).
Smatra se da protein kodiran ovim genom igra važnu ulogu u savijanju proteina i lučenju u ER. Budući da je potiskivanje proteina povezano s ubrzanom apoptozom, također se sugerira da ima važnu citoprotektivnu ulogu u ćelijskim poremećajima uzrokovanim hipoksijom. Pokazalo se da je ovaj protein nadreguliran u tumorima, posebno u tumorima dojke, pa je povezan s invazivnošću tumora.
Postoji i alternativno mjesto za translaciju ovog gena kojem nedostaje signalni peptid. Ovaj protein bez signalnog peptida je samo tri aminokiseline kraći od zrelog proteina u ER-u, a smatra se da ima funkciju održavanja u citosolu.[7]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 999 aminokiselina, a molekulska težina 111.335 Da.[8].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MADKVRRQRP | RRRVCWALVA | VLLADLLALS | DTLAVMSVDL | GSESMKVAIV | ||||
| KPGVPMEIVL | NKESRRKTPV | IVTLKENERF | FGDSAASMAI | KNPKATLRYF | ||||
| QHLLGKQADN | PHVALYQARF | PEHELTFDPQ | RQTVHFQISS | QLQFSPEEVL | ||||
| GMVLNYSRSL | AEDFAEQPIK | DAVITVPVFF | NQAERRAVLQ | AARMAGLKVL | ||||
| QLINDNTATA | LSYGVFRRKD | INTTAQNIMF | YDMGSGSTVC | TIVTYQMVKT | ||||
| KEAGMQPQLQ | IRGVGFDRTL | GGLEMELRLR | ERLAGLFNEQ | RKGQRAKDVR | ||||
| ENPRAMAKLL | REANRLKTVL | SANADHMAQI | EGLMDDVDFK | AKVTRVEFEE | ||||
| LCADLFERVP | GPVQQALQSA | EMSLDEIEQV | ILVGGATRVP | RVQEVLLKAV | ||||
| GKEELGKNIN | ADEAAAMGAV | YQAAALSKAF | KVKPFVVRDA | VVYPILVEFT | ||||
| REVEEEPGIH | SLKHNKRVLF | SRMGPYPQRK | VITFNRYSHD | FNFHINYGDL | ||||
| GFLGPEDLRV | FGSQNLTTVK | LKGVGDSFKK | YPDYESKGIK | AHFNLDESGV | ||||
| LSLDRVESVF | ETLVEDSAEE | ESTLTKLGNT | ISSLFGGGTT | PDAKENGTDT | ||||
| VQEEEESPAE | GSKDEPGEQV | ELKEEAEAPV | EDGSQPPPPE | PKGDATPEGE | ||||
| KATEKENGDK | SEAQKPSEKA | EAGPEGVAPA | PEGEKKQKPA | RKRRMVEEIG | ||||
| VELVVLDLPD | LPEDKLAQSV | QKLQDLTLRD | LEKQEREKAA | NSLEAFIFET | ||||
| QDKLYQPEYQ | EVSTEEQREE | ISGKLSAAST | WLEDEGVGAT | TVMLKEKLAE | ||||
| LRKLCQGLFF | RVEERKKWPE | RLSALDNLLN | HSSMFLKGAR | LIPEMDQIFT | ||||
| EVEMTTLEKV | INETWAWKNA | TLAEQAKLPA | TEKPVLLSKD | IEAKMMALDR | ||||
| EVQYLLNKAK | FTKPRPRPKD | KNGTRAEPPL | NASASDQGEK | VIPPAGQTED | ||||
| AEPISEPEKV | ETGSEPGDTE | PLELGGPGAE | PEQKEQSTGQ | KRPLKNDEL |
Reference uredi
- ^ a b c ENSG00000149428 GRCh38: Ensembl release 89: ENSG00000280682, ENSG00000149428 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000032115 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Ikeda J, Kaneda S, Kuwabara K, Ogawa S, Kobayashi T, Matsumoto M, Yura T, Yanagi H (Feb 1997). "Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150". Biochem Biophys Res Commun. 230 (1): 94–9. doi:10.1006/bbrc.1996.5890. PMID 9020069.
- ^ Ozawa K, Kuwabara K, Tamatani M, Takatsuji K, Tsukamoto Y, Kaneda S, Yanagi H, Stern DM, Eguchi Y, Tsujimoto Y, Ogawa S, Tohyama M (Mar 1999). "150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death". J Biol Chem. 274 (10): 6397–404. doi:10.1074/jbc.274.10.6397. PMID 10037731.
- ^ a b "Entrez Gene: HYOU1 hypoxia up-regulated 1".
- ^ "UniProt, Q9Y4L1". Pristupljeno 23. 8. 2021.
Dopunska literatura uredi
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- Kitao Y, Matsuyama T, Takano K, et al. (2007). "Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?". Antioxid. Redox Signal. 9 (5): 589–95. doi:10.1089/ars.2006.1518. PMID 17330988.
- Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.