Kalpain-7

Kalpain-7
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2QFE
Identifikatori
Aliasi	CAPN7
Vanjski ID-jevi	OMIM: 606400 MGI: 1338030 HomoloGene: 7651 GeneCards: CAPN7
Lokacija gena (čovjek)
Hrom.	Hromosom 3 (čovjek)
Kraj	15,252,916 bp
Lokacija gena (miš)
Hrom.	Hromosom 14 (miš)
Kraj	31,093,943 bp
Ontologija gena
Molekularna funkcija	• MIT domain binding; • GO:0070122 peptidase activity; • endopeptidase activity; • cysteine-type peptidase activity; • GO:0001948, GO:0016582 vezivanje za proteine; • hydrolase activity; • calcium-dependent cysteine-type endopeptidase activity;
Ćelijska komponenta	• Egzosom; • intracellular anatomical structure; • jedro; • nukleoplazma; • citoplazma; • citosol; • nuclear body;
Biološki proces	• positive regulation of epithelial cell migration; • self proteolysis; • Proteoliza;
	Izvori:Amigo / QuickGO
Ortolozi
	23473
	12339
	ENSG00000131375
	ENSMUSG00000021893
	Q9Y6W3; Q7Z479
	Q9R1S8
	NM_014296
	NM_009796; NM_001360187
NP_055111; NP_001363015; NP_001363016; NP_001363018; NP_001363019;
	NP_001363020; NP_001363022; NP_055111.1
	NP_033926; NP_001347116
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Kalpain-7 (PALB, homolog Aspergillus nidulans, PALBH) jest protein koji je kod ljudi kodiran genom CAPN7 sa p kraka hromosoma 3, sekvenca p25.1. Genomske koordinate su u rasponu : 3:15,206,246-15,252,916. (prema NCBI).

Kalpain je unutarćelijska, nelizosomna cisteinska proteaza čija je aktivnost regulisana kalcijem. Mikro- i milikanalni bolovi (CAPN1, 114220 i CAPN2, 114230), koji se razlikuju po potrebi za kalcijem za aktivnost proteaze, su sveprisutno izraženi. Ostali kalpaini su eksprimirani u specifičnim tkivima, kao što su CAPN3 (114240) i CAPN9 (606401), koji su dominantni u skeletnim mišićima i gastrointestinalnom tkivu (Huang i Wang, 2001).^[5]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 813 aminokiselina, a molekulska težina 92.652 Da.

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MDATALERDA	VQFARLAVQR	DHEGRYSEAV	FYYKEAAQAL	IYAEMAGSSL
ENIQEKITEY	LERVQALHSA	VQSKSADPLK	SKHQLDLERA	HFLVTQAFDE
DEKENVEDAI	ELYTEAVDLC	LKTSYETADK	VLQNKLKQLA	RQALDRAEAL
SEPLTKPVGK	ISSTSVKPKP	PPVRAHFPLG	ANPFLERPQS	FISPQSCDAQ
GQRYTAEEIE	VLRTTSKING	IEYVPFMNVD	LRERFAYPMP	FCDRWGKLPL
SPKQKTTFSK	WVRPEDLTNN	PTMIYTVSSF	SIKQTIVSDC	SFVASLAISA
AYERRFNKKL	ITGIIYPQNK	DGEPEYNPCG	KYMVKLHLNG	VPRKVIIDDQ
LPVDHKGELL	CSYSNNKSEL	WVSLIEKAYM	KVMGGYDFPG	SNSNIDLHAL
TGWIPERIAM	HSDSQTFSKD	NSFRMLYQRF	HKGDVLITAS	TGMMTEAEGE
KWGLVPTHAY	AVLDIREFKG	LRFIQLKNPW	SHLRWKGRYS	ENDVKNWTPE
LQKYLNFDPR	TAQKIDNGIF	WISWDDLCQY	YDVIYLSWNP	GLFKESTCIH
STWDAKQGPV	KDAYSLANNP	QYKLEVQCPQ	GGAAVWVLLS	RHITDKDDFA
NNREFITMVV	YKTDGKKVYY	PADPPPYIDG	IRINSPHYLT	KIKLTTPGTH
TFTLVVSQYE	KQNTIHYTVR	VYSACSFTFS	KIPSPYTLSK	RINGKWSGQS
AGGCGNFQET	HKNNPIYQFH	IEKTGPLLIE	LRGPRQYSVG	FEVVTVSTLG
DPGPHGFLRK	SSGDYRCGFC	YLELENIPSG	IFNIIPSTFL	PKQEGPFFLD
FNSIIPIKIT	QLQ

Funkcija

Pretraživanjem baze podataka EST za homologe atipskog Aspergillus-ovog kalpaina Palb, praćenog RACE, Futai et al. (2001) su dobili cDNK koji kodiraju mišji i ljudski CAPN7, koji su nazvali PALBH. Izvedeni ljudski protein od 814 aminokiselina sadrži domen cisteinske proteaze sličan kalpainu i C-terminalni PBH domen. Northern blot analiza je otkrila široku ekspresiju 3,8-kb CAPN7 transkripta. Ekspresija je bila jaka u pankreasu, umjerena u skeletnim mišićima, jetri i placenti, a slaba u bubrezima, plućima, mozgu i srcu. Western blot analiza otkrila je ekspresiju proteina od 92 kD. Imunofluorescentna mikroskopija pokazala je jedarnu ekspresiju CAPN7, za razliku od drugih kalpaina. Funkcionalna analiza nije mogla pokazati komplementaciju u mutantnom kvascu ili aktivnost protiv konvencijskih supstrata kalpaina.^[6]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000131375 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000021893 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Huang, Y., Wang, K. K. W. The calpain family and human disease. Trends Molec. Med. 7: 355-362, 2001. PubMed: 11516996.
^ Futai, E., Kubo, T., Sorimachi, H., Suzuki, K., Maeda, T. Molecular cloning of PalBH, a mammalian homologue of the Aspergillus atypical calpain PalB. Biochim. Biophys. Acta 1517: 316-319, 2001. PubMed: 11342116.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000131375 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000021893 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[5] Huang, Y., Wang, K. K. W. The calpain family and human disease. Trends Molec. Med. 7: 355-362, 2001. PubMed: 11516996.

[6] Futai, E., Kubo, T., Sorimachi, H., Suzuki, K., Maeda, T. Molecular cloning of PalBH, a mammalian homologue of the Aspergillus atypical calpain PalB. Biochim. Biophys. Acta 1517: 316-319, 2001. PubMed: 11342116.

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