TIMM9

TIMM9
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2BSK
Identifikatori
Aliasi	TIMM9
Vanjski ID-jevi	OMIM: 607384 MGI: 1353436 HomoloGene: 40847 GeneCards: TIMM9
Lokacija gena (čovjek)
Hrom.	Hromosom 14 (čovjek)
Kraj	58,427,531 bp
Lokacija gena (miš)
Hrom.	Hromosom 12 (miš)
Kraj	71,183,458 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje iona cinka; • chaperone binding; • GO:0001948, GO:0016582 vezivanje za proteine; • protein homodimerization activity; • vezivanje iona metala; • transporter activity;
Ćelijska komponenta	• mitochondrial inner membrane; • mitochondrial intermembrane space; • mitochondrial intermembrane space protein transporter complex; • membrana; • mitohondrija; • TIM22 mitochondrial import inner membrane insertion complex;
Biološki proces	• protein transport; • protein insertion into mitochondrial inner membrane; • protein targeting to mitochondrion; • sluh;
	Izvori:Amigo / QuickGO
Ortolozi
	26520
	30056
	ENSG00000100575
	ENSMUSG00000021079
	Q9Y5J7
	Q9WV98
NM_001304485; NM_001304486; NM_001304487; NM_001304488; NM_001304489;
	NM_001304490; NM_001304491; NM_012460
	NM_001024853; NM_001024854; NM_001286203; NM_013896
NP_001291414; NP_001291415; NP_001291416; NP_001291417; NP_001291418;
	NP_001291419; NP_001291420; NP_036592
	NP_001020024; NP_001020025; NP_001273132; NP_038924
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Podjedinica Tim9 mitohondrijske importne unutrašnjomembranske translokaze je enzim koji je kod ljudi kodiran genom TIMM9.^[5]^[6]^[7]

TIMM9 pripada porodici evolucijski konzerviranih proteina, koji su organizirani u heterooligomerne komplekse u mitohondrijskom intermembranskom prostoru. Ovi proteini posreduju pri importu i inserciji proteina hidrofobne membrane u unutrašnju membranu mitohondrija.^[7]

Analizom sekvenci i floorescentnom hibridizacijom in situ.^[8] Bauer et al. (1999) lociraali su gen TIMM9 na hromosomu 14q21, analizom hibridne radijacije.^[9]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 89 aminokiselina, a molekulska težina 10.378 Da^[10].

10		20		30		40		50
MAAQIPESDQ		IKQFKEFLGT		YNKLTETCFL		DCVKDFTTRE		VKPEETTCSE
HCLQKYLKMT		QRISMRFQEY		HIQQNEALAA		KAGLLGQPR

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Pretražujući bazu podataka sa ljudskim i kvasac|S. pombe]] TIMM8A sekvenci kao upiti, Jin et al. (1999) identificirali su nekoliko novih članova porodice TIM, uključujući TIMM9. Protein TIMM9 sadrži 89 aminokiselina. Northern blot analiza otkrila je sveprisutnu ekspresiju transkripata TIMM9 od 1,2 i 2,4 kb u tkivima odraslih, s najvećom ekspresijom u skeletnim i srčanom mišiću i u gušterači.

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000100575 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000021079 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Jin H, Kendall E, Freeman TC, Roberts RG, Vetrie DL (Feb 2000). "The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins". Genomics. 61 (3): 259–67. doi:10.1006/geno.1999.5966. PMID 10552927.
^ Muhlenbein N, Hofmann S, Rothbauer U, Bauer MF (Mar 2004). "Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria". J Biol Chem. 279 (14): 13540–6. doi:10.1074/jbc.M312485200. PMID 14726512.
^ ^a ^b "Entrez Gene: TIMM9 translocase of inner mitochondrial membrane 9 homolog (yeast)".
^ Jin et bal. (1999) mapirali su gen TIMM9 na hromosomu 14, pozicija 14q22.3-q24. Jin, H., Kendall, E., Freeman, T. C., Roberts, R. G., Vetrie, D. L. P. The human family of deafness/dystonia peptide (DDP) related mitochondrial import proteins. Genomics 61: 259-267, 1999. PubMed: 10552927
^ Bauer, M. F., Rothbauer, U., Muhlenbein, N., Smith, R. J. H., Gerbitz, K.-D., Neupert, W., Brunner, M., Hofmann, S. The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom. FEBS Lett. 464: 41-47, 1999. PubMed: 10611480
^ "UniProt, Q9Y5J7". Pristupljeno 11. 9. 2017.

Dopunska literatura

Webb CT, Gorman MA, Lazarou M, et al. (2006). "Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller". Mol. Cell. 21 (1): 123–33. doi:10.1016/j.molcel.2005.11.010. PMID 16387659.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Vial S, Lu H, Allen S, et al. (2002). "Assembly of Tim9 and Tim10 into a functional chaperone". J. Biol. Chem. 277 (39): 36100–8. doi:10.1074/jbc.M202310200. PMID 12138093.
Rothbauer U, Hofmann S, Mühlenbein N, et al. (2001). "Role of the deafness dystonia peptide 1 (DDP1) in import of human Tim23 into the inner membrane of mitochondria". J. Biol. Chem. 276 (40): 37327–34. doi:10.1074/jbc.M105313200. PMID 11489896.
Bauer MF, Rothbauer U, Mühlenbein N, et al. (2000). "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom". FEBS Lett. 464 (1–2): 41–7. doi:10.1016/S0014-5793(99)01665-8. PMID 10611480.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000100575 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000021079 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10552927-5] Jin H, Kendall E, Freeman TC, Roberts RG, Vetrie DL (Feb 2000). "The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins". Genomics. 61 (3): 259–67. doi:10.1006/geno.1999.5966. PMID 10552927.

[pmid14726512-6] Muhlenbein N, Hofmann S, Rothbauer U, Bauer MF (Mar 2004). "Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria". J Biol Chem. 279 (14): 13540–6. doi:10.1074/jbc.M312485200. PMID 14726512.

[entrez-7] "Entrez Gene: TIMM9 translocase of inner mitochondrial membrane 9 homolog (yeast)".

[8] Jin et bal. (1999) mapirali su gen TIMM9 na hromosomu 14, pozicija 14q22.3-q24. Jin, H., Kendall, E., Freeman, T. C., Roberts, R. G., Vetrie, D. L. P. The human family of deafness/dystonia peptide (DDP) related mitochondrial import proteins. Genomics 61: 259-267, 1999. PubMed: 10552927

[9] Bauer, M. F., Rothbauer, U., Muhlenbein, N., Smith, R. J. H., Gerbitz, K.-D., Neupert, W., Brunner, M., Hofmann, S. The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom. FEBS Lett. 464: 41-47, 1999. PubMed: 10611480

[UniProt-10] "UniProt, Q9Y5J7". Pristupljeno 11. 9. 2017.

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