PRMT6

Proteinska arginin N-metiltransferaza 6 jest enzim koji je kod ljudi kodiran genom PRMT6 sa hromosoma 1.

PRMT6
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 4HC4, 4QPP, 4QQK, 4Y2H, 4Y30, 5EGS, 5E8R, 5HZM
Identifikatori
Aliasi	PRMT6
Vanjski ID-jevi	OMIM: 608274 MGI: 2139971 HomoloGene: 10024 GeneCards: PRMT6
EC broj	2.1.1.321
Lokacija gena (čovjek) Hrom. Hromosom 1 (čovjek) Bend 1p13.3 Početak 107,056,674 bp Kraj 107,067,636 bp
Lokacija gena (miš) Hrom. Hromosom 3 (miš) Bend 3|3 F3 Početak 110,153,425 bp Kraj 110,158,314 bp
Ontologija gena Molekularna funkcija • GO:0102674, GO:0102675 methyltransferase activity • aktivnost sa transferazom • histone methyltransferase activity • GO:0016275 protein-arginine omega-N monomethyltransferase activity • histone methyltransferase activity (H4-R3 specific) • GO:0031493 histone binding • protein-arginine omega-N asymmetric methyltransferase activity • chromatin binding • histone methyltransferase activity (H2A-R3 specific) • histone methyltransferase activity (H3-R2 specific) • GO:0001948, GO:0016582 vezivanje za proteine • protein-arginine N-methyltransferase activity • histone-arginine N-methyltransferase activity Ćelijska komponenta • nukleoplazma • jedro • Jedarce • citosol Biološki proces • GO:0009373 regulation of transcription, DNA-templated • GO:1901227 negative regulation of transcription by RNA polymerase II • transcription, DNA-templated • cellular response to DNA damage stimulus • Ćelijsko starenje • Metilacija • peptidyl-arginine methylation, to asymmetrical-dimethyl arginine • Metilacija histona • GO:0022415 viral process • GO:0045996 negative regulation of transcription, DNA-templated • GO:0100026 Popravka DNK • base-excision repair • protein methylation • histone H4-R3 methylation • regulation of megakaryocyte differentiation • GO:0031497, GO:0006336, GO:0034724, GO:0001301, GO:0007580, GO:0034652, GO:0010847 chromatin organization • histone H3-R2 methylation • peptidyl-arginine N-methylation • negative regulation of histone H3-K4 methylation • regulation of signal transduction by p53 class mediator • histone arginine methylation Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	55170	99890
Ensembl	ENSG00000198890	ENSMUSG00000049300
UniProt	Q96LA8	Q6NZB1
RefSeq (mRNK)	NM_018137	NM_178891
RefSeq (bjelančevina)	NP_060607	NP_849222
Lokacija (UCSC)	Chr 1: 107.06 – 107.07 Mb	Chr 3: 110.15 – 110.16 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 375 aminokiselina, a molekulska težina 41.938 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSQPKKRKLE		SGGGGEGGEG		TEEEDGAERE		AALERPRRTK		RERDQLYYEC
YSDVSVHEEM		IADRVRTDAY		RLGILRNWAA		LRGKTVLDVG		AGTGILSIFC
AQAGARRVYA		VEASAIWQQA		REVVRFNGLE		DRVHVLPGPV		ETVELPEQVD
AIVSEWMGYG		LLHESMLSSV		LHARTKWLKE		GGLLLPASAE		LFIAPISDQM
LEWRLGFWSQ		VKQHYGVDMS		CLEGFATRCL		MGHSEIVVQG		LSGEDVLARP
QRFAQLELSR		AGLEQELEAG		VGGRFRCSCY		GSAPMHGFAI		WFQVTFPGGE
SEKPLVLSTS		PFHPATHWKQ		ALLYLNEPVQ		VEQDTDVSGE		ITLLPSRDNP
RRLRVLLRYK		VGDQEEKTKD		FAMED

Funkcija

Proteinske arginin N-metiltransferaze, kao što je PRMT6, kataliziraju sekvencni prijenos metil-grupe od S-adenozil-L-metionina na dušike argininskih ostataka bočnog lanca unutar proteina, kako bi se formirao metilirani derivat arginina i S-adenozil-L-homocistein (prema OMIM]

Dopunska literatura

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• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Boulanger MC, Liang C, Russell RS, et al. (2005). "Methylation of Tat by PRMT6 Regulates Human Immunodeficiency Virus Type 1 Gene Expression". J. Virol. 79 (1): 124–31. doi:10.1128/JVI.79.1.124-131.2005. PMC 538702. PMID 15596808.
• Sgarra R, Lee J, Tessari MA, et al. (2006). "The AT-hook of the chromatin architectural transcription factor high mobility group A1a is arginine-methylated by protein arginine methyltransferase 6". J. Biol. Chem. 281 (7): 3764–72. doi:10.1074/jbc.M510231200. PMID 16293633.
• Willemsen NM, Hitchen EM, Bodetti TJ, et al. (2007). "Protein methylation is required to maintain optimal HIV-1 infectivity". Retrovirology. 3: 92. doi:10.1186/1742-4690-3-92. PMC 1766367. PMID 17169163.
• Invernizzi CF, Xie B, Richard S, Wainberg MA (2007). "PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA". Retrovirology. 3: 93. doi:10.1186/1742-4690-3-93. PMC 1779295. PMID 17176473.
• Xie B, Invernizzi CF, Richard S, Wainberg MA (2007). "Arginine Methylation of the Human Immunodeficiency Virus Type 1 Tat Protein by PRMT6 Negatively Affects Tat Interactions with both Cyclin T1 and the Tat Transactivation Region". J. Virol. 81 (8): 4226–34. doi:10.1128/JVI.01888-06. PMC 1866113. PMID 17267505.
• Guccione E, Bassi C, Casadio F, et al. (2007). "Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive". Nature. 449 (7164): 933–7. Bibcode:2007Natur.449..933G. doi:10.1038/nature06166. PMID 17898714. S2CID 4341782.