MGAT5
Alfa-1,6-manozilglikoprotein 6-beta-N-acetilglukozaminiltransferaza A jest enzim koji je kod ljudi kodiran genom MGAT5 sa hromosoma 2.[5][6]
Amiokiselininska sekvenca
urediDužina polipeptidnog lanca je 741 aminokiselina, a molekulska težina 84.543 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MALFTPWKLS | SQKLGFFLVT | FGFIWGMMLL | HFTIQQRTQP | ESSSMLREQI | ||||
| LDLSKRYIKA | LAEENRNVVD | GPYAGVMTAY | DLKKTLAVLL | DNILQRIGKL | ||||
| ESKVDNLVVN | GTGTNSTNST | TAVPSLVALE | KINVADIING | AQEKCVLPPM | ||||
| DGYPHCEGKI | KWMKDMWRSD | PCYADYGVDG | STCSFFIYLS | EVENWCPHLP | ||||
| WRAKNPYEEA | DHNSLAEIRT | DFNILYSMMK | KHEEFRWMRL | RIRRMADAWI | ||||
| QAIKSLAEKQ | NLEKRKRKKV | LVHLGLLTKE | SGFKIAETAF | SGGPLGELVQ | ||||
| WSDLITSLYL | LGHDIRISAS | LAELKEIMKK | VVGNRSGCPT | VGDRIVELIY | ||||
| IDIVGLAQFK | KTLGPSWVHY | QCMLRVLDSF | GTEPEFNHAN | YAQSKGHKTP | ||||
| WGKWNLNPQQ | FYTMFPHTPD | NSFLGFVVEQ | HLNSSDIHHI | NEIKRQNQSL | ||||
| VYGKVDSFWK | NKKIYLDIIH | TYMEVHATVY | GSSTKNIPSY | VKNHGILSGR | ||||
| DLQFLLRETK | LFVGLGFPYE | GPAPLEAIAN | GCAFLNPKFN | PPKSSKNTDF | ||||
| FIGKPTLREL | TSQHPYAEVF | IGRPHVWTVD | LNNQEEVEDA | VKAILNQKIE | ||||
| PYMPYEFTCE | GMLQRINAFI | EKQDFCHGQV | MWPPLSALQV | KLAEPGQSCK | ||||
| QVCQESQLIC | EPSFFQHLNK | DKDMLKYKVT | CQSSELAKDI | LVPSFDPKNK | ||||
| HCVFQGDLLL | FSCAGAHPRH | QRVCPCRDFI | KGQVALCKDC | L |
Funkcija
urediOvaj gen kodira manozil (alfa-1,6-)-glikoprotein beta-1,6-N-acetil-glukozaminiltransferazu, glikoziltransferazu uključenu u sintezu proteina vezanih za i lipid-vezane oligosaharide. Promjenama oligosaharida na površini ćelije, glikoproteini izazivaju značajne promene u adheziji ili migracijskom ponašanju ćelije. Povećanje aktivnosti kodiranog proteina može biti u korelaciji s progresijom invazivnih malignosti.[6]
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000152127 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036155 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Saito H, Nishikawa A, Gu J, Ihara Y, Soejima H, Wada Y, Sekiya C, Niikawa N, Taniguchi N (Feb 1994). "cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+". Biochem Biophys Res Commun. 198 (1): 318–27. doi:10.1006/bbrc.1994.1045. PMID 8292036.
- ^ a b "Entrez Gene: MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase".
- ^ "UniProt, Q09328" (jezik: engleski). Pristupljeno 18. 11. 2021.
Dopunska literatura
uredi- Dennis JW, Granovsky M, Warren CE (2000). "Glycoprotein glycosylation and cancer progression". Biochim. Biophys. Acta. 1473 (1): 21–34. doi:10.1016/s0304-4165(99)00167-1. PMID 10580127.
- Buckhaults P, Chen L, Fregien N, Pierce M (1997). "Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene". J. Biol. Chem. 272 (31): 19575–81. doi:10.1074/jbc.272.31.19575. PMID 9235963.
- Park C, Jin UH, Lee YC, et al. (1999). "Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B". Arch. Biochem. Biophys. 367 (2): 281–8. doi:10.1006/abbi.1999.1252. PMID 10395745.
- Granovsky M, Fata J, Pawling J, et al. (2000). "Suppression of tumor growth and metastasis in Mgat5-deficient mice". Nat. Med. 6 (3): 306–12. doi:10.1038/73163. PMID 10700233. S2CID 1950342.
- Ihara S, Miyoshi E, Ko JH, et al. (2002). "Prometastatic effect of N-acetylglucosaminyltransferase V is due to modification and stabilization of active matriptase by adding beta 1-6 GlcNAc branching". J. Biol. Chem. 277 (19): 16960–7. doi:10.1074/jbc.M200673200. PMID 11864986.
- Saito T, Miyoshi E, Sasai K, et al. (2002). "A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity". J. Biol. Chem. 277 (19): 17002–8. doi:10.1074/jbc.M200521200. PMID 11872751.
- Dosaka-Akita H, Miyoshi E, Suzuki O, et al. (2004). "Expression of N-acetylglucosaminyltransferase v is associated with prognosis and histology in non-small cell lung cancers". Clin. Cancer Res. 10 (5): 1773–9. doi:10.1158/1078-0432.CCR-1047-3. PMID 15014031.
- Guo P, Zhang Y, Shen ZH, et al. (2004). "Effect of N-acetylglucosaminyltransferase V on the expressions of other glycosyltransferases". FEBS Lett. 562 (1–3): 93–8. doi:10.1016/S0014-5793(04)00188-7. PMID 15044007. S2CID 42049140.
- Kim CH (2005). "Increased expression of N-acetylglucosaminyltransferase-V in human hepatoma cells by retinoic acid and 1alpha,25-dihydroxyvitamin D3". Int. J. Biochem. Cell Biol. 36 (11): 2307–19. doi:10.1016/j.biocel.2004.04.021. PMID 15313475.
- Tomiie M, Isaka S, Miyoshi E, et al. (2005). "Elevated expression of N-acetylglucosaminyltransferase V in first trimester human placenta". Biochem. Biophys. Res. Commun. 330 (3): 999–1004. doi:10.1016/j.bbrc.2005.02.186. PMID 15809094.
- Fang H, Huang W, Xu YY, et al. (2006). "Blocking of N-acetylglucosaminyltransferase V induces cellular endoplasmic reticulum stress in human hepatocarcinoma 7,721 cells". Cell Res. 16 (1): 82–92. doi:10.1038/sj.cr.7310011. PMID 16467879.
- Ishimura H, Takahashi T, Nakagawa H, et al. (2006). "N-acetylglucosaminyltransferase V and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer". Clin. Cancer Res. 12 (8): 2506–11. doi:10.1158/1078-0432.CCR-05-1938. PMID 16638859.
- Wang L, Liang Y, Li Z, et al. (2007). "Increase in beta1-6 GlcNAc branching caused by N-acetylglucosaminyltransferase V directs integrin beta1 stability in human hepatocellular carcinoma cell line SMMC-7721". J. Cell. Biochem. 100 (1): 230–41. doi:10.1002/jcb.21071. PMID 16924681. S2CID 11808116.
- Li J, Wang XM, Wang Q, et al. (2007). "Down-regulation of N-acetylglucosaminyltransferase-V induces ER stress by changing glycosylation and function of GLUT1". Arch. Biochem. Biophys. 463 (1): 102–9. doi:10.1016/j.abb.2007.03.005. PMID 17451637.
- Kudo T, Nakagawa H, Takahashi M, et al. (2007). "N-glycan alterations are associated with drug resistance in human hepatocellular carcinoma". Mol. Cancer. 6 (1): 32. doi:10.1186/1476-4598-6-32. PMC 1878497. PMID 17488527.