Kalumenin
Kalumenin je protein koji je kod ljudi kodiran genom CALU.[5][6]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 315 aminokiselina, а molekulska težina 37.107 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MDLRQFLMCL | SLCTAFALSK | PTEKKDRVHH | EPQLSDKVHN | DAQSFDYDHD | ||||
| AFLGAEEAKT | FDQLTPEESK | ERLGKIVSKI | DGDKDGFVTV | DELKDWIKFA | ||||
| QKRWIYEDVE | RQWKGHDLNE | DGLVSWEEYK | NATYGYVLDD | PDPDDGFNYK | ||||
| QMMVRDERRF | KMADKDGDLI | ATKEEFTAFL | HPEEYDYMKD | IVVQETMEDI | ||||
| DKNADGFIDL | EEYIGDMYSH | DGNTDEPEWV | KTEREQFVEF | RDKNRDGKMD | ||||
| KEETKDWILP | SDYDHAEAEA | RHLVYESDQN | KDGKLTKEEI | VDKYDLFVGS | ||||
| QATDFGEALV | RHDEF |
Funkcija uredi
Kalumenin (CALU) je protein koji veže kalcij lokaliziran u endoplazmatskom retikulumu (ER) i uključen je u takve funkcije ER, kao što su savijanje proteina i sortiranje. Kalumenin je član natporodice EF-šaka u ER i Golgijevom aparatu pod imenom CERC. CERC je akronim za članove svoje porodice Cab-45, retikulokalbin, Erc-55 (RCN2) i kalumenin. Gen CALU kodira izvedeni protein od 315 aminokiselina, koji sadrži šesr motiva EF-šake, jedno potencijalno mjesto N-glikozilacije i C-terminalni ER signal zadržavanja. CALU proteini čovjeka i miša 98% su identični. CALU iRNK se sveprisutno eksprimira u ljudskim tkivima i mapira nasekvenci 7q32.[6]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000128595 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029767 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Yabe D, Taniwaki M, Nakamura T, Kanazawa N, Tashiro K, Honjo T (Aug 1998). "Human calumenin gene (CALU): cDNA isolation and chromosomal mapping to 7q32". Genomics. 49 (2): 331–3. doi:10.1006/geno.1998.5245. PMID 9598325.
- ^ a b "Entrez Gene: CALU calumenin".
- ^ "UniProt, O43852" (jezik: engleski). Pristupljeno 23. 9. 2021.
Dopunska literatura uredi
- Yabe D, Nakamura T, Kanazawa N, et al. (1997). "Calumenin, a Ca2+-binding protein retained in the endoplasmic reticulum with a novel carboxyl-terminal sequence, HDEF". J. Biol. Chem. 272 (29): 18232–9. doi:10.1074/jbc.272.29.18232. PMID 9218460.
- Vorum H, Liu X, Madsen P, et al. (1998). "Molecular cloning of a cDNA encoding human calumenin, expression in Escherichia coli and analysis of its Ca2+-binding activity". Biochim. Biophys. Acta. 1386 (1): 121–31. doi:10.1016/s0167-4838(98)00089-2. PMID 9675259.
- Hseu MJ, Yen CH, Tzeng MC (1999). "Crocalbin: a new calcium-binding protein that is also a binding protein for crotoxin, a neurotoxic phospholipase A2". FEBS Lett. 445 (2–3): 440–4. doi:10.1016/S0014-5793(99)00177-5. PMID 10094503. S2CID 40129100.
- Vorum H, Hager H, Christensen BM, et al. (1999). "Human calumenin localizes to the secretory pathway and is secreted to the medium". Exp. Cell Res. 248 (2): 473–81. doi:10.1006/excr.1999.4431. PMID 10222138.
- Vorum H, Jacobsen C, Honoré B (2000). "Calumenin interacts with serum amyloid P component". FEBS Lett. 465 (2–3): 129–34. doi:10.1016/S0014-5793(99)01734-2. PMID 10631319. S2CID 1943508.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
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- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Basrur V, Yang F, Kushimoto T, et al. (2003). "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins". J. Proteome Res. 2 (1): 69–79. doi:10.1021/pr025562r. PMID 12643545.
- Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
- Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767–72. Bibcode:2003Sci...300..767S. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
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- Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
- Chi A, Valencia JC, Hu ZZ, et al. (2007). "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes". J. Proteome Res. 5 (11): 3135–44. doi:10.1021/pr060363j. PMID 17081065.
Vanjski linkovi uredi
- Lokacija ljudskog genoma CALU i stranica sa detaljima o genu CALU u UCSC Genome Browseru.