HMG20A

HMG20A
Identifikatori
Aliasi	HMG20A
Vanjski ID-jevi	OMIM: 605534 MGI: 1914117 HomoloGene: 32399 GeneCards: HMG20A
Lokacija gena (čovjek)
Hrom.	Hromosom 15 (čovjek)
Kraj	77,485,607 bp
Lokacija gena (miš)
Hrom.	Hromosom 9 (miš)
Kraj	56,404,220 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity; • protein heterodimerization activity; • vezivanje sa DNK; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje identičnih proteina; • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific;
Ćelijska komponenta	• jedro;
Biološki proces	• GO:0009373 regulation of transcription, DNA-templated; • GO:1901227 negative regulation of transcription by RNA polymerase II; • negative regulation of neuron differentiation; • transcription, DNA-templated; • negative regulation of protein sumoylation; • GO:0031497, GO:0006336, GO:0034724, GO:0001301, GO:0007580, GO:0034652, GO:0010847 chromatin organization;
	Izvori:Amigo / QuickGO
Ortolozi
	10363
	66867
	ENSG00000140382
	ENSMUSG00000032329
	Q9NP66
	Q9DC33
	NM_001304504; NM_001304505; NM_018200
	NM_025812
	NP_001291433; NP_001291434; NP_060670
	NP_080088
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Visokomobilni protein grupe 20A je protein koji je kod ljudi kodiran genom HMG20A.^[5]^[6]

Sumoy et al. (2000) potvrdili su mapiranje gena HMG20A u hromosomu 15q24, fluoroscentnom in situ hibridizacijom i hibridnom radijacijskom analizom.^[7]

Kloniranje i ekspresija

Pretragom baze podataka EST pomoću sekvence dobijene iz klona na hromosomu 15q24, Sumoy et al. (2000) identificirali su novog člana porodice HMG, kojeg su označili kao HMG20A. cDNK HMG20A pune dužine kodira izvedeni protein od 347 aminokiselina.^[7] Northern blot analizom otkriveni su dvas glavna transkripta od približno 4 i 9 kb i jedva uočljivi transkript od 1,5 kb. Transkript od 4 kb eksprimira se gotovo posvuda, sa najvećom ekspresijom u slezeni, sjemenicima i srcu i nižim nivoima u leukocitima periferne krvi. Ekspresija mozga je uglavnom jednolična, sa većom ekspresijom u malom mozgu i nižim nivoima u kičmenoj moždini u subtalamusnom jezgru. Transkript od 9 kb različito se eksprimira u slezini, testisima, leukocitima i mozgu i jedva ga je moguće otkriti u drugim tkivima.

Pored toga, Sumoy et al. (2000) utvrdili su da gen HMG20A sadrži 10 egzona.^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 47 aminokiselina, a molekulska težina 40.144 Da.^[8].

10	20	30	40	50
MENLMTSSTL	PPLFADEDGS	KESNDLATTG	LNHPEVPYSS	GATSSTNNPE
FVEDLSQGQL	LQSESSNAAE	GNEQRHEDEQ	RSKRGGWSKG	RKRKKPLRDS
NAPKSPLTGY	VRFMNERREQ	LRAKRPEVPF	PEITRMLGNE	WSKLPPEEKQ
RYLDEADRDK	ERYMKELEQY	QKTEAYKVFS	RKTQDRQKGK	SHRQDAARQA
THDHEKETEV	KERSVFDIPI	FTEEFLNHSK	AREAELRQLR	KSNMEFEERN
AALQKHVESM	RTAVEKLEVD	VIQERSRNTV	LQQHLETLRQ	VLTSSFASMP
LPGSGETPTV	DTIDSYMNRL	HSIILANPQD	NENFIATVRE	VVNRLDR

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000140382 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032329 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Sumoy L, Carim L, Escarceller M, Nadal M, Gratacòs M, Pujana MA, Estivill X, Peral B (Jun 2000). "HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of HMG-box genes with ubiquitous expression". Cytogenetics and Cell Genetics. 88 (1–2): 62–7. doi:10.1159/000015486. PMID 10773667.
^ "Entrez Gene: HMG20A high-mobility group 20A".
^ ^a ^b ^c Sumoy, L., Carim, L., Escarceller, M., Nadal, M., Gratacos, M., Pujana, M. A., Estivill, X., Peral, B. (2000). "HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of HMG-box genes with ubiquitous expression". Cytogenet. Cell Genet. Zanemaren tekst "pages 62-67" (pomoć); Zanemaren tekst "PubMed: 10773667" (pomoć); Nepoznati parametar |Volume= zanemaren (prijedlog zamjene: |volume=) (pomoć)CS1 održavanje: upotreba parametra authors (link)
^ "UniProt, Q9NP66". Pristupljeno 14. 7. 2021.

Dopunska literatura

Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (juli 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (oktobar 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Maruyama K, Sugano S (januar 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

V anjski linkovi

HMG20A lokacija ljudskog genoma UCSC Genome Browser.

HMG20A detalji ljudskog genoma u UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000140382 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000032329 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10773667-5] Sumoy L, Carim L, Escarceller M, Nadal M, Gratacòs M, Pujana MA, Estivill X, Peral B (Jun 2000). "HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of HMG-box genes with ubiquitous expression". Cytogenetics and Cell Genetics. 88 (1–2): 62–7. doi:10.1159/000015486. PMID 10773667.

[entrez-6] "Entrez Gene: HMG20A high-mobility group 20A".

[Sumoy-7] Sumoy, L., Carim, L., Escarceller, M., Nadal, M., Gratacos, M., Pujana, M. A., Estivill, X., Peral, B. (2000). "HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of HMG-box genes with ubiquitous expression". Cytogenet. Cell Genet. Zanemaren tekst "pages 62-67" (pomoć); Zanemaren tekst "PubMed: 10773667" (pomoć); Nepoznati parametar |Volume= zanemaren (prijedlog zamjene: |volume=) (pomoć)CS1 održavanje: upotreba parametra authors (link)

[UniProt-8] "UniProt, Q9NP66". Pristupljeno 14. 7. 2021.

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