DHX32
Putativna faktorska preradna pre-iRNK ATP-ovisna RNK helikaza DHX32 je enzim koji je kod ljudi kodiran genom DHX32.[5]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 743 aminokiseline, а molekulska težina 84.419[6]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MEEEGLECPN | SSSEKRYFPE | SLDSSDGDEE | EVLACEDLEL | NPFDGLPYSS | ||||
| RYYKLLKERE | DLPIWKEKYS | FMENLLQNQI | VIVSGDAKCG | KSAQVPQWCA | ||||
| EYCLSIHYQH | GGVICTQVHK | QTVVQLALRV | ADEMDVNIGH | EVGYVIPFEN | ||||
| CCTNETILRY | CTDDMLQREM | MSNPFLGSYG | VIILDDIHER | SIATDVLLGL | ||||
| LKDVLLARPE | LKLIINSSPH | LISKLNSYYG | NVPVIEVKNK | HPVEVVYLSE | ||||
| AQKDSFESIL | RLIFEIHHSG | EKGDIVVFLA | CEQDIEKVCE | TVYQGSNLNP | ||||
| DLGELVVVPL | YPKEKCSLFK | PLDETEKRCQ | VYQRRVVLTT | SSGEFLIWSN | ||||
| SVRFVIDVGV | ERRKVYNPRI | RANSLVMQPI | SQSQAEIRKQ | ILGSSSSGKF | ||||
| FCLYTEEFAS | KDMTPLKPAE | MQEANLTSMV | LFMKRIDIAG | LGHCDFMNRP | ||||
| APESLMQALE | DLDYLAALDN | DGNLSEFGII | MSEFPLDPQL | SKSILASCEF | ||||
| DCVDEVLTIA | AMVTAPNCFS | HVPHGAEEAA | LTCWKTFLHP | EGDHFTLISI | ||||
| YKAYQDTTLN | SSSEYCVEKW | CRDYFLNCSA | LRMADVIRAE | LLEIIKRIEL | ||||
| PYAEPAFGSK | ENTLNIKKAL | LSGYFMQIAR | DVDGSGNYLM | LTHKQVAQLH | ||||
| PLSGYSITKK | MPEWVLFHKF | SISENNYIRI | TSEISPELFM | QLVPQYYFSN | ||||
| LPPSESKDIL | QQVVDHLSPV | STMNKEQQMC | ETCPETEQRC | TLQ |
Funkcija uredi
Proteini iz kutije DEAD, karakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), su pretpostavljene RNK-helikaze. Uključeni su u brojne ćelijske procese koji obuhvataju promjenu sekundarne strukture RNK, poput translacijske inicijacije, jedarne i mitohondrijske prerade i sklapanje ribosoma i splajsosoma. Na osnovu njihovih obrazaca distribucije, vjeruje se da su neki članovi ove porodice proteina DEAD-kutije uključeni u embriogenezu, spermatogenezu, te ćelijski rast i diobu. Ovaj gen kodira člana ove porodice čija funkija nije utvrđena. Alternativna prerada ovog gena generira dvije varijante transkripta, ali priroda cijele dužine jedne od varijanti nije definirana.[5]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000089876 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000030986 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: DHX32 DEAH (Asp-Glu-Ala-His) box polypeptide 32".
- ^ "UniProt, Q7L7V1". Pristupljeno 31. 8. 2021.
Dopunska literatura uredi
- Alli Z, Chen Y, Abdul Wajid S, et al. (2007). "A role for DHX32 in regulating T-cell apoptosis". Anticancer Res. 27 (1A): 373–7. PMID 17352256.
- Chen Y, Alli Z, Ackerley C, et al. (2007). "Altered distribution of heat shock protein 60 (Hsp60) with dysregulated expression of DHX32". Exp. Mol. Pathol. 82 (3): 256–61. doi:10.1016/j.yexmp.2006.11.002. PMID 17174952.
- Alli Z, Ackerley C, Chen Y, et al. (2007). "Nuclear and mitochondrial localization of the putative RNA helicase DHX32". Exp. Mol. Pathol. 81 (3): 245–8. doi:10.1016/j.yexmp.2006.07.005. PMID 16959245.
- Alli Z, Nam EH, Beimnet K, Abdelhaleem M (2006). "The activation-induced expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells". Cell. Immunol. 237 (2): 141–6. doi:10.1016/j.cellimm.2005.12.003. PMID 16414036.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Abdelhaleem M (2002). "The novel helicase homologue DDX32 is down-regulated in acute lymphoblastic leukemia". Leuk. Res. 26 (10): 945–54. doi:10.1016/S0145-2126(02)00040-1. PMID 12163057.
- Liu J, Yuan Y, Huan J, Shen Z (2001). "Inhibition of breast and brain cancer cell growth by BCCIPalpha, an evolutionarily conserved nuclear protein that interacts with BRCA2". Oncogene. 20 (3): 336–45. doi:10.1038/sj.onc.1204098. PMID 11313963.