DHX16

Pretpostavljeni pre-iRNK-prerađujući faktor ATP-ovisne RNK helikaze DHX16 jest enzim koji je kod ljudi kodiran genom DHX16.

DHX16
Identifikatori
Aliasi	DHX16
Vanjski ID-jevi	OMIM: 603405 MGI: 1916442 HomoloGene: 2658 GeneCards: DHX16
Lokacija gena (čovjek) Hrom. Hromosom 6 (čovjek) Bend 6p21.33 Početak 30,653,119 bp Kraj 30,673,006 bp
Lokacija gena (miš) Hrom. Hromosom 17 (miš) Bend 17|17 B1 Početak 36,190,711 bp Kraj 36,203,562 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • RNA helicase activity • nucleotide binding • GO:0001948, GO:0016582 vezivanje za proteine • ATP binding • GO:0008026 helicase activity • hydrolase activity • nucleic acid binding • ATPase activity • vezivanje sa RNK • GO:0034459 3'-5' RNA helicase activity Ćelijska komponenta • spliceosomal complex • jedro • citoplazma • nukleoplazma • U2-type precatalytic spliceosome Biološki proces • mRNA processing • Prerada RNK • mRNA splicing, via spliceosome • metabolizam Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	8449	69192
Ensembl	ENSG00000233561 ENSG00000206486 ENSG00000233418 ENSG00000231377 ENSG00000227222 ENSG00000204560 ENSG00000233049 ENSG00000226171	ENSMUSG00000024422
UniProt	O60231 Q5SQH5	n/a
RefSeq (mRNK)	NM_001164239 NM_003587 NM_001363515	NM_026987
RefSeq (bjelančevina)	NP_001157711 NP_003578 NP_001350444	n/a
Lokacija (UCSC)	Chr 6: 30.65 – 30.67 Mb	Chr 17: 36.19 – 36.2 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 1.041 aminokiselina, а molekulska težina 119.264 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MATPAGLERW		VQDELHSVLG		LSERHVAQFL		IGTAQRCTSA		EEFVQRLRDT
DTLDLSGPAR		DFALRLWNKV		PRKAVVEKPA		RAAEREARAL		LEKNRSYRLL
EDSEESSEET		VSRAGSSLQK		KRKKRKHLRK		KREEEEEEEA		SEKGKKKTGG
SKQQTEKPES		EDEWERTERE		RLQDLEERDA		FAERVRQRDK		DRTRNVLERS
DKKAYEEAQK		RLKMAEEDRK		AMVPELRKKS		RREYLAKRER		EKLEDLEAEL
ADEEFLFGDV		ELSRHERQEL		KYKRRVRDLA		REYRAAGEQE		KLEATNRYHM
PKETRGQPAR		AVDLVEEESG		APGEEQRRWE		EARLGAASLK		FGARDAASQE
PKYQLVLEEE		ETIEFVRATQ		LQGDEEPSAP		PTSTQAQQKE		SIQAVRRSLP
VFPFREELLA		AIANHQVLII		EGETGSGKTT		QIPQYLFEEG		YTNKGMKIAC
TQPRRVAAMS		VAARVAREMG		VKLGNEVGYS		IRFEDCTSER		TVLRYMTDGM
LLREFLSEPD		LASYSVVMVD		EAHERTLHTD		ILFGLIKDVA		RFRPELKVLV
ASATMDTARF		STFFDDAPVF		RIPGRRFPVD		IFYTKAPEAD		YLEACVVSVL
QIHVTQPPGD		ILVFLTGQEE		IEAACEMLQD		RCRRLGSKIR		ELLVLPIYAN
LPSDMQARIF		QPTPPGARKV		VVATNIAETS		LTIEGIIYVL		DPGFCKQKSY
NPRTGMESLT		VTPCSKASAN		QRAGRAGRVA		AGKCFRLYTA		WAYQHELEET
TVPEIQRTSL		GNVVLLLKSL		GIHDLMHFDF		LDPPPYETLL		LALEQLYALG
ALNHLGELTT		SGRKMAELPV		DPMLSKMILA		SEKYSCSEEI		LTVAAMLSVN
NSIFYRPKDK		VVHADNARVN		FFLPGGDHLV		LLNVYTQWAE		SGYSSQWCYE
NFVQFRSMRR		ARDVREQLEG		LLERVEVGLS		SCQGDYIRVR		KAITAGYFYH
TARLTRSGYR		TVKQQQTVFI		HPNSSLFEQQ		PRWLLYHELV		LTTKEFMRQV
LEIESSWLLE		VAPHYYKAKE		LEDPHAKKMP		KKIGKTREEL		G

Funkcija

Proteini iz DEAD-kutije karakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), pretpostavljene su RNK helikaze. Oni su uključeni u brojne ćelijske procese promjena sekundarne strukture RNK, poput iniciranja translacije, jedarne i mitohondrijske prerade i sastavljanja ribosoma i splajsosoma. Na osnovu njihovih obrazaca distribucije, vjeruje se da su neki članovi ove porodice uključeni u embriogenezu, spermatogenezu, ćelijski rast i diobu. Ovaj gen kodira DEAD-kutijski protein, koji je funkcionalni homolog proteina fisionog kvasca Prp8, uključen u napredovanje ćelijskog ciklusa. Ovaj gen je mapiran u MHC regiji na heomosomu 6, sekvenca p21.3, regiji u kojoj su povezani mnogi geni malignih, genetičkih i autoimunskih bolesti.

Dopunska literatura

• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Nagase T, Ishikawa K, Miyajima N, et al. (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.