DGLUCY
D-glutamat ciklaza, skraćeno DGLUCY, je protein koji je kod ljudi kodiran genom DGLUCY.[5]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 616 aminokiselina, a molekulska težina 66.437 Da.[6].
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MPFTLHLRSR | LPSAIRSLIL | QKKPNIRNTS | SMAGELRPAS | LVVLPRSLAP | ||||
| AFERFCQVNT | GPLPLLGQSE | PEKWMLPPQG | AISETRMGHP | QFWKYEFGAC | ||||
| TGSLASLEQY | SEQLKDMVAF | FLGCSFSLEE | ALEKAGLPRR | DPAGHSQTTV | ||||
| PCVTHAGFCC | PLVVTMRPIP | KDKLEGLVRA | CCSLGGEQGQ | PVHMGDPELL | ||||
| GIKELSKPAY | GDAMVCPPGE | VPVFWPSPLT | SLGAVSSCET | PLAFASIPGC | ||||
| TVMTDLKDAK | APPGCLTPER | IPEVHHISQD | PLHYSIASVS | ASQKIRELES | ||||
| MIGIDPGNRG | IGHLLCKDEL | LKASLSLSHA | RSVLITTGFP | THFNHEPPEE | ||||
| TDGPPGAVAL | VAFLQALEKE | VAIIVDQRAW | NLHQKIVEDA | VEQGVLKTQI | ||||
| PILTYQGGSV | EAAQAFLCKN | GDPQTPRFDH | LVAIERAGRA | ADGNYYNARK | ||||
| MNIKHLVDPI | DDLFLAAKKI | PGISSTGVGD | GGNELGMGKV | KEAVRRHIRH | ||||
| GDVIACDVEA | DFAVIAGVSN | WGGYALACAL | YILYSCAVHS | QYLRKAVGPS | ||||
| RAPGDQAWTQ | ALPSVIKEEK | MLGILVQHKV | RSGVSGIVGM | EVDGLPFHNT | ||||
| HAEMIQKLVD | VTTAQV |
Ortolozi uredi
Ljudski gen, DGLUCY, visoko je konzerviran kod sisara i ptica.[7] Ortolozi iz pretraživanja baza BLAST i BLAT otkrivaju da je ljudska DGLUCY iRNK sekvenca konzervirana sa identitetom sekvence od 98% u čimpanze, 88% u miševa, i 81% u kljunara i kokoši.[8][9] Sljedeća tabela sadrži popis ortologa prikupljenih iz pretraživanja BLAST-a. Poravnavanje sekvence izvedeno je pomoću blastn za dobivanje identiteta sekvence, rezultata i E-vrijednosti između iRNK ljudske c14orf159 varijante 1 i njenih ortologa..
| Rod i vrsta | Uobičajeno ime | Broj pristula u bazu NCBI | Dužina sekvence (bp) | Identitet sekvence | Rezultat | E-vrijednost |
|---|---|---|---|---|---|---|
| Homo sapiens | Čovjek | NM_001102366 | 3164 | 100% | 0 | |
| Pan troglodytes | Čimpanze | XM_510121 | 2974 | 98% | 4281 | 0 |
| Mus musculus | Miš | NM_145448 | 3231 | 88% | 495 | 0 |
| Ornithorhynchus anatinus | Kljunar | XM_00154336.1 | 1962 | 81% | 217 | 0 |
| Gallus gallus | Kokoš | XM_421319 | 3389 | 81 | 50 | 0 |
Utvrđeno je da je protein koji kodira ljudski gen DGLUCY visoko konzerviran među sisarima, pticama, vodozemcima, ribama, tunikatama, cnidariama i ehinodermatama. Međutim, nisu pronađeni proteinski ortolozi u nematoda, arthropoda, [[gljiva]]ma, protistima, biljkama, bakterijama ili arhejama. Gljive i bakterije sadrže konzervirani domen DUF1445 koji se nalazi u ljudskom c14orf159 i njegovim ortolozima. Pretrage BLAST-a i BLAT korištene su za pronalaženje ortologa proteina c14orf159. Sljedeća tabela navodi ortologe proteina za ljudski protein s identitetom sekvence, sličnošću sekvence, rezultatima i E-vrijednostima izvedenim iz usporedbe blastp sekvenci.[10]
| Rod i vrsta | Uobičajeno ime | Broj pristula u bazu NCBI | Dužina sekvence (bp) | Identitet sekvence | Rezultat | E-vrijednost | |
|---|---|---|---|---|---|---|---|
| Homo sapiens | Čovjek | NP_001095839.1 | 564 | 100% | 100% | 0 | |
| Pan troglodytes | Čimpanza | XP_510121.2 | 724 | 557/621 (89%) | 561/621 (90%) | 1109 | 0 |
| Ailuropoda melanoleuca | Panda | EFB15996.1 | 585 | 413/585 (70%) | 461/585 (78%) | 824 | 0 |
| Rattus norvegicus | Rat | XP_343096.2 | 618 | 423/618 (68%) | 470/618 (76%) | 774 | 0 |
| Mus musculus | Miš | NP_663423.2 | 617 | 414/623 (66%) | 468/621 (75%) | 796 | 0 |
| Equus caballus | Konj | XP_001916913.1 | 581 | 390/585 (66%) | 433/585 (74%) | 728 | 6E-115 |
| Ornithorhynchus anatinus | Kljunar | XP_001514386.1 | 653 | 358/628 (57%) | 443/628 (70%) | 696 | 0 |
| Gallus gallus | Kokoš | XP_421319.2 | 617 | 330/614 (53%) | 414/614 (67%) | 630 | 0 |
| Xenopus tropicalis | Zapadna kandžasta žaba | CAJ82045.1 | 616 | 302/611 (49%) | 399/611 (65%) | 582 | 1E-170 |
| Danio rerio | Zebrafish | AAI244131.1 | 621 | 284/607 (46%) | 386/607 (63%) | 530 | 6E-155 |
| Branchiostoma floridae | Lanceleta | XP_002612376.1 | 615 | 237/611 (38%) | 334/611 (54%) | 397 | 6E-115 |
| Ciona intestinalis | Tunikata | XP_001173256 | 486 | 161/501 (32%) | 241/501 (48%) | 244 | 5E-69 |
| Strongylocentrotus purpuratus | Kalifornijski ljubičasti morski jež | XP_782739.1 | 631 | 9/33 (27%) | 15/33 (45%) | 320 | 5E-87 |
| Nematostella vectensis | Starletna morska anemona | XP_001637867 | 529 | 134/501 (26%) | 211/501 (42%) | 120 | 1E-31 |
Posttranslacijska modifikacija uredi
Proteinski proizvod gena DGLUCY predviđen je [5] i pronađen [11][12] translocitrani protein ciljanja u mitohondrijama.
Za protein DGLUCY predviđaju se i posttranslacijske modifikacije. Sve predviđene lokacije u ljudskom DGLUCY-u su upoređene sa ortolouzima, koristeći poravnavanje višestrukih sekvenci, kako bi se utvrdila vjerovatnoća modifikacije.[13][14][15][16] [17]
Regulacija uredi
Estrogeni receptor alfa, u prisustvu estradiola, veže se za gen DGLUCY i vjerovatno regulira njegovu ekspresiju.[18]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000133943 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000021185 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: C14orf159 chromosome 14 open reading frame 159".
- ^ "UniProt, Q7Z3D6". Pristupljeno 23. 7. 2021.
- ^ BLAST. NCBI. accessed 19 April 2010. http://blast.ncbi.nlm.nih.gov/Blast.cgi
- ^ UCSC Genome Browser website, BLAT. accessed 10 April 2010.
- ^ BLAST. NCBI. accessed 19 April 2010.
- ^ Blastp. NCBI. http://blast.ncbi.nlm.nih.gov/Blast.cgi
- ^ http://www.dkfz.de/LIFEdb/(2ij04255g3tubn45u1j3kl45)/Table.aspx[mrtav link] | title = RZPD CloneID DKFZp686J0759 | vauthors = Mehrle A, Rosenfelder H | work = LifeDB: Database for Localization, Interaction, Functional assays and Expression of Proteins| publisher = German Cancer Research Center }}
- ^ Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (oktobar 2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- ^ Prediction of glycosylation across the human proteome and the correlation to protein function. Gupta, R. and S. Brunak. Pacific Symposium on Biocomputing, 7:310-322, 2002 <http://www.cbs.dtu.dk/services/YinOYang/>.
- ^ Locating proteins in the cell using TargetP, SignalP, and related tools Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen Nature Protocols 2, 953-971 (2007) http://www.cbs.dtu.dk/services/SignalP/.
- ^ Scanning the available Dictyostelium discoideum proteome for O-linked GlcNAc glycosylation sites using neural networks. R. Gupta, E. Jung, A.A. Gooley, K.L. Williams, S. Brunak and J. Hansen. Glycobiology: 9(10):1009-22, 1999 http://www.cbs.dtu.dk/services/DictyOGlyc/.
- ^ Analysis and prediction of mammalian protein glycation. Morten Bo Johansen, Lars Kiemer and Søren Brunak Glycobiology, 16:844-853, 2006 http://www.cbs.dtu.dk/services/NetGlycate/.
- ^ Sulfinator. Expasy tools. 2010. http://expasy.org/tools/sulfinator/.
- ^ Creekmore AL, Ziegler YS, Bonéy JL, Nardulli AM (mart 2007). "Estrogen receptor α regulates expression of the breast cancer 1 associated ring domain 1 (BARD1) gene through intronic DNA sequence". Mol. Cell. Endocrinol. 267 (1–2): 106–15. doi:10.1016/j.mce.2007.01.001. PMC 1933484. PMID 17275994.
Vasnjski linkovi uredi
- Lokacija ljudskog genoma C14orf159 i stranica sa detaljima o genu C14orf159 u UCSC Genome Browseru.
Dopunska literatura uredi
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Clark HF, Gurney AL, Abaya E, et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Cheng J, Kapranov P, Drenkow J, et al. (2005). "Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution". Science. 308 (5725): 1149–54. Bibcode:2005Sci...308.1149C. doi:10.1126/science.1108625. PMID 15790807.
- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.