DDX23

Vjerovatno ATP-ovisna RNK-helikaza DDX23 je enzim koji je kod ljudi kodiran genom DDX23.

DDX23
Dostupne strukture PDB Pretraga Human UniProta: PDBe RCSB Spisak PDB ID kodova 4NHO, 3JCR
Identifikatori
Aliasi	DDX23
Vanjski ID-jevi	OMIM: 612172 MGI: 1921601 HomoloGene: 3542 GeneCards: DDX23
Lokacija gena (čovjek) Hrom. Hromosom 12 (čovjek) Bend 12q13.12 Početak 48,829,756 bp Kraj 48,852,842 bp
Lokacija gena (miš) Hrom. Hromosom 15 (miš) Bend 15|15 F1 Početak 98,543,015 bp Kraj 98,560,775 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • nucleotide binding • GO:0008026 helicase activity • GO:0001948, GO:0016582 vezivanje za proteine • nucleic acid binding • hydrolase activity • ATP binding • vezivanje sa RNK Ćelijska komponenta • catalytic step 2 spliceosome • U5 snRNP • nukleoplazma • spliceosomal complex • Egzosom • jedro • Jedarce • citoplazma Biološki proces • mRNA splicing, via spliceosome • RNA splicing, via transesterification reactions • mRNA processing • RNA secondary structure unwinding • Prerada RNK • cis assembly of pre-catalytic spliceosome Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	9416	74351
Ensembl	ENSG00000174243	ENSMUSG00000003360
UniProt	Q9BUQ8	n/a
RefSeq (mRNK)	NM_004818	NM_001080981
RefSeq (bjelančevina)	NP_004809	n/a
Lokacija (UCSC)	Chr 12: 48.83 – 48.85 Mb	Chr 15: 98.54 – 98.56 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Ovaj gen kodira člana porodice proteina DEAD-boksa. Proteini izove kutije, okarakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), su pretpostavljene RNK-helikaze. Uključeni suu brojne ćelijske procese u promjeni sekundarne strukture RNK, kao što je iniciranje translacije, jedarna i mitohondrijska prerada, te sastavljanje ribosoma i splajsosoma. Na osnovu njihovih obrazaca distribucije, vjeruje se da su neki članovi ove porodice uključeni u embriogenezu, spermatogenezu i ćelijski rast i diobu. Protein kodiran ovim genom je komponenta kompleksa U5 snRNP; može olakšati konformacijske promjene u splajsosomu tokom prerade jedarne pre-iRNK. Alternativno prerađena varijanta transkripta pronađena je za ovaj gen, ali njena biološka valjanost nije utvrđena.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 820 aminokiselina, a molekulska težina 95.583 Da..

10		20		30		40		50
MAGELADKKD		RDASPSKEER		KRSRTPDRER		DRDRDRKSSP		SKDRKRHRSR
DRRRGGSRSR		SRSRSKSAER		ERRHKERERD		KERDRNKKDR		DRDKDGHRRD
KDRKRSSLSP		GRGKDFKSRK		DRDSKKDEED		EHGDKKPKAQ		PLSLEELLAK
KKAEEEAEAK		PKFLSKAERE		AEALKRRQQE		VEERQRMLEE		ERKKRKQFQD
LGRKMLEDPQ		ERERRERRER		MERETNGNED		EEGRQKIREE		KDKSKELHAI
KERYLGGIKK		RRRTRHLNDR		KFVFEWDASE		DTSIDYNPLY		KERHQVQLLG
RGFIAGIDLK		QQKREQSRFY		GDLMEKRRTL		EEKEQEEARL		RKLRKKEAKQ
RWDDRHWSQK		KLDEMTDRDW		RIFREDYSIT		TKGGKIPNPI		RSWKDSSLPP
HILEVIDKCG		YKEPTPIQRQ		AIPIGLQNRD		IIGVAETGSG		KTAAFLIPLL
VWITTLPKID		RIEESDQGPY		AIILAPTREL		AQQIEEETIK		FGKPLGIRTV
AVIGGISRED		QGFRLRMGCE		IVIATPGRLI		DVLENRYLVL		SRCTYVVLDE
ADRMIDMGFE		PDVQKILEHM		PVSNQKPDTD		EAEDPEKMLA		NFESGKHKYR
QTVMFTATMP		PAVERLARSY		LRRPAVVYIG		SAGKPHERVE		QKVFLMSESE
KRKKLLAILE		QGFDPPIIIF		VNQKKGCDVL		AKSLEKMGYN		ACTLHGGKGQ
EQREFALSNL		KAGAKDILVA		TDVAGRGIDI		QDVSMVVNYD		MAKNIEDYIH
RIGRTGRAGK		SGVAITFLTK		EDSAVFYELK		QAILESPVSS		CPPELANHPD
AQHKPGTILT		KKRREETIFA

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Dopunska literatura

• Achsel T, Ahrens K, Brahms H, et al. (1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Mol. Cell. Biol. 18 (11): 6756–66. doi:10.1128/mcb.18.11.6756. PMC 109259. PMID 9774689.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
• Irobi J, Nelis E, Verhoeven K, et al. (2002). "Mutation analysis of 12 candidate genes for distal hereditary motor neuropathy type II (distal HMN II) linked to 12q24.3". J. Peripher. Nerv. Syst. 7 (2): 87–95. doi:10.1046/j.1529-8027.2002.02014.x. PMID 12090300. S2CID 8453412.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.