AKT3

AKT3
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2X18
Identifikatori
Aliasi	AKT3
Vanjski ID-jevi	OMIM: 611223 MGI: 1345147 HomoloGene: 55904 GeneCards: AKT3
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	243,851,079 bp
Lokacija gena (miš)
Hrom.	Hromosom 1 (miš)
Kraj	177,085,769 bp
Obrazac RNK ekspresije
	; ; ; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• aktivnost sa transferazom; • protein kinase activity; • nucleotide binding; • protein serine/threonine kinase activity; • GO:0001948, GO:0016582 vezivanje za proteine; • ATP binding; • kinase activity;
Ćelijska komponenta	• intracellular anatomical structure; • membrana; • jedro; • citoplazma;
Biološki proces	• protein phosphorylation; • GO:0007243 intracellular signal transduction; • GO:0035404 peptidyl-serine phosphorylation; • mitochondrial genome maintenance; • GO:0072468 Transdukcija signala; • Fosforilacija; • positive regulation of TOR signaling; • positive regulation of cell size; • brain morphogenesis; • homeostasis of number of cells within a tissue; • positive regulation of endothelial cell proliferation; • positive regulation of blood vessel endothelial cell migration; • positive regulation of angiogenesis; • positive regulation of cell migration involved in sprouting angiogenesis; • positive regulation of vascular endothelial cell proliferation; • positive regulation of artery morphogenesis; • negative regulation of cellular senescence;
	Izvori:Amigo / QuickGO
Ortolozi
	10000
	23797
	ENSG00000117020; ENSG00000275199
	ENSMUSG00000019699
	Q9Y243
	Q9WUA6
	NM_001206729; NM_005465; NM_181690; NM_001370074
	NM_011785
	NP_001193658; NP_005456; NP_859029; NP_001357003
	NP_035915
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

RAC-gama serin/treonin-protein kinaza jest enzim koji je kod ljudi kodiran genom AKT3 sa hromosoma 1.^[5]^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 479 aminokiselina, a molekulska težina 55.775 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MSDVTIVKEG	WVQKRGEYIK	NWRPRYFLLK	TDGSFIGYKE	KPQDVDLPYP
LNNFSVAKCQ	LMKTERPKPN	TFIIRCLQWT	TVIERTFHVD	TPEEREEWTE
AIQAVADRLQ	RQEEERMNCS	PTSQIDNIGE	EEMDASTTHH	KRKTMNDFDY
LKLLGKGTFG	KVILVREKAS	GKYYAMKILK	KEVIIAKDEV	AHTLTESRVL
KNTRHPFLTS	LKYSFQTKDR	LCFVMEYVNG	GELFFHLSRE	RVFSEDRTRF
YGAEIVSALD	YLHSGKIVYR	DLKLENLMLD	KDGHIKITDF	GLCKEGITDA
ATMKTFCGTP	EYLAPEVLED	NDYGRAVDWW	GLGVVMYEMM	CGRLPFYNQD
HEKLFELILM	EDIKFPRTLS	SDAKSLLSGL	LIKDPNKRLG	GGPDDAKEIM
RHSFFSGVNW	QDVYDKKLVP	PFKPQVTSET	DTRYFDEEFT	AQTITITPPE
KYDEDGMDCM	DNERRPHFPQ	FSYSASGRE

Funkcija

Protein koji je kodiran ovim genom je član potporodice AKT serin/treonin protein kinaza. Poznato je da su AKT-kinaze regulatori ćelijske signalizacije kao odgovor na insulin i faktore rasta. Oni su uključeni u širok spektar bioloških procesa uključujući proliferaciju ćelija, diferencijaciju, apoptozu, tumorigenezu, kao i sintezu glikogena i unos glukoze. Pokazalo se da je ova kinaza stimulirana faktoru rasta izvedenom iz trombocita (PDGF), insulinom i insulinolikim faktorom rasta (IGF1). Opisane su alternativno prerađene varijante transkripta koje kodiraju različite izoforme.^[8] Miševi kojima nedostaje Akt3 imaju normalan metabolizam glukoze (bez dijabetesa), imaju približno normalnu tjelesnu težinu, ali imaju 25% smanjenje mase mozga. Inače, Akt3 je visoko eksprimiran u mozgu.

Interakcije

Pokazalo se da je AKT3 u interakciji sa rotein-kinazom Mζ.^[9]

Reference

^ ^a ^b ^c ENSG00000275199 GRCh38: Ensembl release 89: ENSG00000117020, ENSG00000275199 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000019699 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Brodbeck D, Cron P, Hemmings BA (Apr 1999). "A human protein kinase Bgamma with regulatory phosphorylation sites in the activation loop and in the C-terminal hydrophobic domain". J Biol Chem. 274 (14): 9133–6. doi:10.1074/jbc.274.14.9133. PMID 10092583.
^ Nakatani K, Sakaue H, Thompson DA, Weigel RJ, Roth RA (Jun 1999). "Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site". Biochem Biophys Res Commun. 257 (3): 906–10. doi:10.1006/bbrc.1999.0559. PMID 10208883.
^ "UniProt, Q9Y243" (jezik: en.). Pristupljeno 7. 12. 2021.CS1 održavanje: nepoznati jezik (link)
^ "Entrez Gene: AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)".
^ Hodgkinson CP, Sale EM, Sale GJ (2002). "Characterization of PDK2 activity against protein kinase B gamma". Biochemistry. 41 (32): 10351–9. doi:10.1021/bi026065r. PMID 12162751.

Dopunska literatura

Li W, Zhang J, Bottaro DP, Pierce JH (1997). "Identification of serine 643 of protein kinase C-delta as an important autophosphorylation site for its enzymatic activity". J. Biol. Chem. 272 (39): 24550–5. doi:10.1074/jbc.272.39.24550. PMID 9305920.
Borgatti P, Zauli G, Colamussi ML, Gibellini D, Previati M, Cantley LL, Capitani S (1998). "Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells". Eur. J. Immunol. 27 (11): 2805–11. doi:10.1002/eji.1830271110. PMID 9394803. S2CID 6303471.
Walker KS, Deak M, Paterson A, Hudson K, Cohen P, Alessi DR (1998). "Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha". Biochem. J. 331 ( Pt 1) (Pt 1): 299–308. doi:10.1042/bj3310299. PMC 1219352. PMID 9512493.
Nakatani K, Thompson DA, Barthel A, Sakaue H, Liu W, Weigel RJ, Roth RA (1999). "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines". J. Biol. Chem. 274 (31): 21528–32. doi:10.1074/jbc.274.31.21528. PMID 10419456.
Masure S, Haefner B, Wesselink JJ, Hoefnagel E, Mortier E, Verhasselt P, Tuytelaars A, Gordon R, Richardson A (1999). "Molecular cloning, expression and characterization of the human serine/threonine kinase Akt-3". Eur. J. Biochem. 265 (1): 353–60. doi:10.1046/j.1432-1327.1999.00774.x. PMID 10491192.
Murthy SS, Tosolini A, Taguchi T, Testa JR (2000). "Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization". Cytogenet. Cell Genet. 88 (1–2): 38–40. doi:10.1159/000015481. PMID 10773662. S2CID 556711.
Meucci O, Fatatis A, Simen AA, Miller RJ (2000). "Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival". Proc. Natl. Acad. Sci. U.S.A. 97 (14): 8075–80. Bibcode:2000PNAS...97.8075M. doi:10.1073/pnas.090017497. PMC 16672. PMID 10869418.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Zauli G, Milani D, Mirandola P, Mazzoni M, Secchiero P, Miscia S, Capitani S (2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway". FASEB J. 15 (2): 483–91. doi:10.1096/fj.00-0354com. PMID 11156964. S2CID 26315564.
Kapasi AA, Fan S, Singhal PC (2001). "Role of 14-3-3epsilon, c-Myc/Max, and Akt phosphorylation in HIV-1 gp 120-induced mesangial cell proliferation". Am. J. Physiol. Renal Physiol. 280 (2): F333–42. doi:10.1152/ajprenal.2001.280.2.F333. PMID 11208609. S2CID 29992656.
Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Sandirasegarane L, Kester M (2001). "Enhanced stimulation of Akt-3/protein kinase B-gamma in human aortic smooth muscle cells". Biochem. Biophys. Res. Commun. 283 (1): 158–63. doi:10.1006/bbrc.2001.4739. PMID 11322783.
Brodbeck D, Hill MM, Hemmings BA (2001). "Two splice variants of protein kinase B gamma have different regulatory capacity depending on the presence or absence of the regulatory phosphorylation site serine 472 in the carboxyl-terminal hydrophobic domain". J. Biol. Chem. 276 (31): 29550–8. doi:10.1074/jbc.M104633200. PMID 11387345.
Mende I, Malstrom S, Tsichlis PN, Vogt PK, Aoki M (2001). "Oncogenic transformation induced by membrane-targeted Akt2 and Akt3". Oncogene. 20 (32): 4419–23. doi:10.1038/sj.onc.1204486. PMID 11466625.
Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR (2001). "AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon". Clin. Cancer Res. 7 (8): 2475–9. PMID 11489829.
Laine J, Künstle G, Obata T, Noguchi M (2002). "Differential regulation of Akt kinase isoforms by the members of the TCL1 oncogene family". J. Biol. Chem. 277 (5): 3743–51. doi:10.1074/jbc.M107069200. PMID 11707444.
Deregibus MC, Cantaluppi V, Doublier S, Brizzi MF, Deambrosis I, Albini A, Camussi G (2002). "HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells". J. Biol. Chem. 277 (28): 25195–202. doi:10.1074/jbc.M200921200. PMID 11994280.
Hodgkinson CP, Sale EM, Sale GJ (2002). "Characterization of PDK2 activity against protein kinase B gamma". Biochemistry. 41 (32): 10351–9. doi:10.1021/bi026065r. PMID 12162751.

Vanjski linkovi

AKT3 lokacija ljudskog genoma UCSC Genome Browser.

AKT3 detalji ljudskog genoma u UCSC Genome Browser.

Portal Biologija

[refGRCh38Ensembl-1] ENSG00000275199 GRCh38: Ensembl release 89: ENSG00000117020, ENSG00000275199 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000019699 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10092583-5] Brodbeck D, Cron P, Hemmings BA (Apr 1999). "A human protein kinase Bgamma with regulatory phosphorylation sites in the activation loop and in the C-terminal hydrophobic domain". J Biol Chem. 274 (14): 9133–6. doi:10.1074/jbc.274.14.9133. PMID 10092583.

[pmid10208883-6] Nakatani K, Sakaue H, Thompson DA, Weigel RJ, Roth RA (Jun 1999). "Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site". Biochem Biophys Res Commun. 257 (3): 906–10. doi:10.1006/bbrc.1999.0559. PMID 10208883.

[UniProt-7] "UniProt, Q9Y243" (jezik: en.). Pristupljeno 7. 12. 2021.CS1 održavanje: nepoznati jezik (link)

[entrez-8] "Entrez Gene: AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)".

[pmid12162751-9] Hodgkinson CP, Sale EM, Sale GJ (2002). "Characterization of PDK2 activity against protein kinase B gamma". Biochemistry. 41 (32): 10351–9. doi:10.1021/bi026065r. PMID 12162751.

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