ZRF1

Član 2 potorodice DnaJ homologa C je protein koji je kod ljudi kodiran genom DNAJC2.

ZRF1
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 2M2E
Identifikatori
Aliasi	DNAJC2
Vanjski ID-jevi	OMIM: 605502 MGI: 99470 HomoloGene: 31656 GeneCards: DNAJC2
Lokacija gena (čovjek) Hrom. Hromosom 7 (čovjek) Bend 7q22.1 Početak 103,312,289 bp Kraj 103,344,830 bp
Lokacija gena (miš) Hrom. Hromosom 5 (miš) Bend 5 A3|5 9.97 cM Početak 21,962,265 bp Kraj 21,990,249 bp
Ontologija gena Molekularna funkcija • vezivanje sa DNK • Hsp70 protein binding • GO:0031493 histone binding • chromatin binding • ATPase activator activity • vezivanje sa RNK • ubiquitin modification-dependent histone binding • GO:0001948, GO:0016582 vezivanje za proteine • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific Ćelijska komponenta • citoplazma • citosol • Jedarna membrana • jedro • nukleoplazma Biološki proces • GO:0060469, GO:0009371 positive regulation of transcription, DNA-templated • regulation of cellular response to heat • Replikacija DNK • 'de novo' cotranslational protein folding • GO:0009373 regulation of transcription, DNA-templated • negative regulation of DNA biosynthetic process • transcription, DNA-templated • positive regulation of ATP-dependent activity • GO:0031497, GO:0006336, GO:0034724, GO:0001301, GO:0007580, GO:0034652, GO:0010847 chromatin organization • GO:0044324, GO:0003256, GO:1901213, GO:0046019, GO:0046020, GO:1900094, GO:0061216, GO:0060994, GO:1902064, GO:0003258, GO:0072212 regulation of transcription by RNA polymerase II Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	27000	22791
Ensembl	ENSG00000105821	ENSMUSG00000029014
UniProt	Q99543	P54103
RefSeq (mRNK)	NM_001129887 NM_014377 NM_001362667 NM_001362668	NM_009584
RefSeq (bjelančevina)	NP_001123359 NP_055192 NP_001349596 NP_001349597	NP_033610
Lokacija (UCSC)	Chr 7: 103.31 – 103.34 Mb	Chr 5: 21.96 – 21.99 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 621 aminokiselina, а molekulska težina 71.996 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MLLLPSAADG		RGTAITHALT		SASTLCQVEP		VGRWFEAFVK		RRNRNASASF
QELEDKKELS		EESEDEELQL		EEFPMLKTLD		PKDWKNQDHY		AVLGLGHVRY
KATQRQIKAA		HKAMVLKHHP		DKRKAAGEPI		KEGDNDYFTC		ITKAYEMLSD
PVKRRAFNSV		DPTFDNSVPS		KSEAKDNFFE		VFTPVFERNS		RWSNKKNVPK
LGDMNSSFED		VDIFYSFWYN		FDSWREFSYL		DEEEKEKAEC		RDERRWIEKQ
NRATRAQRKK		EEMNRIRTLV		DNAYSCDPRI		KKFKEEEKAK		KEAEKKAKAE
AKRKEQEAKE		KQRQAELEAA		RLAKEKEEEE		VRQQALLAKK		EKDIQKKAIK
KERQKLRNSC		KTWNHFSDNE		AERVKMMEEV		EKLCDRLELA		SLQCLNETLT
SCTKEVGKAA		LEKQIEEINE		QIRKEKEEAE		ARMRQASKNT		EKSTGGGGNG
SKNWSEDDLQ		LLIKAVNLFP		AGTNSRWEVI		ANYMNIHSSS		GVKRTAKDVI
GKAKSLQKLD		PHQKDDINKK		AFDKFKKEHG		VVPQADNATP		SERFEGPYTD
FTPWTTEEQK		LLEQALKTYP		VNTPERWEKI		AEAVPGRTKK		DCMKRYKELV
EMVKAKKAAQ		EQVLNASRAK		K

Funkcija

Ovaj gen je član porodice fosfoproteinskih gena M-faze (MPP). Kodira fosfoprotein s J domenom i domenom koji veže Myb DNK, koja se nalazi i u jedru i u citosolu. Protein je sposoban formirati heterodimerni kompleks koji se povezuje s ribosomima, djelujući kao molekulski šaperon za novonastale polipeptidne lance pri izlasku iz ribosoma. Ovaj protein je identificiran kao leukemija-povezani antigen, a ekspresija gena je povećana u leukemijskim blastima. Također, hromosomske aberacije koje uključuju ovaj gen povezane su s primarnim pločastim ćelijama tumora glave i vrata. Ovaj gen ima pseudogen na hromosomu 6. Opisane su alternativno prerađene varijante koje kodiraju različite izoforme proteina; međutim, sve varijante nisu u potpunosti okarakterizirane.

Dopunska literatura

• Resto VA, Caballero OL, Buta MR, et al. (2000). "A putative oncogenic role for MPP11 in head and neck squamous cell cancer". Cancer Res. 60 (19): 5529–35. PMID 11034098.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Greiner J, Ringhoffer M, Taniguchi M, et al. (2003). "Characterization of several leukemia-associated antigens inducing humoral immune responses in acute and chronic myeloid leukemia". Int. J. Cancer. 106 (2): 224–31. doi:10.1002/ijc.11200. PMID 12800198. S2CID 24284888.
• Greiner J, Ringhoffer M, Taniguchi M, et al. (2004). "mRNA expression of leukemia-associated antigens in patients with acute myeloid leukemia for the development of specific immunotherapies". Int. J. Cancer. 108 (5): 704–11. doi:10.1002/ijc.11623. PMID 14696097. S2CID 24565397.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Hundley HA, Walter W, Bairstow S, Craig EA (2005). "Human Mpp11 J protein: ribosome-tethered molecular chaperones are ubiquitous". Science. 308 (5724): 1032–4. doi:10.1126/science.1109247. PMID 15802566. S2CID 24164629.
• Otto H, Conz C, Maier P, et al. (2005). "The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex". Proc. Natl. Acad. Sci. U.S.A. 102 (29): 10064–9. doi:10.1073/pnas.0504400102. PMC 1177401. PMID 16002468.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.