VPS72
Homolog vakuolskog proteina 72 pridruženog sortiranju jest protein koji je kod ljudi kodiran genom VPS72 sa hromosoma 1.[5][6]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca je 364 aminokiselina, a molekulska težina Da. 40 594[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MSLAGGRAPR | KTAGNRLSGL | LEAEEEDEFY | QTTYGGFTEE | SGDDEYQGDQ | ||||
| SDTEDEVDSD | FDIDEGDEPS | SDGEAEEPRR | KRRVVTKAYK | EPLKSLRPRK | ||||
| VNTPAGSSQK | AREEKALLPL | ELQDDGSDSR | KSMRQSTAEH | TRQTFLRVQE | ||||
| RQGQSRRRKG | PHCERPLTQE | ELLREAKITE | ELNLRSLETY | ERLEADKKKQ | ||||
| VHKKRKCPGP | IITYHSVTVP | LVGEPGPKEE | NVDIEGLDPA | PSVSALTPHA | ||||
| GTGPVNPPAR | CSRTFITFSD | DATFEEWFPQ | GRPPKVPVRE | VCPVTHRPAL | ||||
| YRDPVTDIPY | ATARAFKIIR | EAYKKYITAH | GLPPTASALG | PGPPPPEPLP | ||||
| GSGPRALRQK | IVIK |
Modelni organizmi
urediU proučavanju funkcije VPS72 korišteni su modelni organizmi. Uslovna nokaut-mišja linija pod nazivom Vps72tm1a(EUCOMM)Wtsi generirana je u Institutu Wellcome Trust Sanger.[8] > Mužjaci i ženke podvrgnuti su standardizovanom fenotipskom pregledu[9] za otkrivanja efekta delecija.[10][11][12][13] Urađeni su dodatni pregledi: Dubinska imunološka fenotipizacija:[14]
| Svojstvo | Fenotip |
|---|---|
| Svi podaci raspoloživi na.[9][14] | |
| Leukociti periferne krvi 6 sedmica | Normalan |
| Insulin | Normalan |
| Hematologija 6 sedmica | Normalan |
| Vijabilnost homozigota na P14 | Nenormalan |
| Studija recesivne letalnosti | Nenormalan |
| Tjelesna težina | Normalan |
| Neurološka procjena | Normalan |
| Snaga stiska | Normalan |
| Dismorfologija | Normalan |
| Indirektna kalorimetrija | Normalan |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog stabla | Normalan |
| DEXA | Normalan |
| Radiografija | Normalan |
| Morfologija oka | Normalan |
| Klinička hemija | Normalan |
| Hematologija 16 seddmica | Normalan |
| Leukociti periferne krvi 16 sedmica | Normalan |
| Težina srca | Normalan |
| Salmonella infekcija | Normalan |
| Funkcija citotoksičnih T-ćelija | Normalan |
| Imunofenotipizacija slezene | Normalan |
| Imunofenotipizacija mezenternog limfnog čvora | Normalan |
| Imunofenotipizacija koštane srži | Normal |
| Kompozicija epidermne imunosti | Normal |
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000163159 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000008958 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Horikawa I, Tanaka H, Yuasa Y, Suzuki M, Oshimura M (Mar 1995). "Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability". Biochemical and Biophysical Research Communications. 208 (3): 999–1007. doi:10.1006/bbrc.1995.1433. PMID 7702631.
- ^ "Entrez Gene: VPS72 vacuolar protein sorting 72 homolog (S. cerevisiae)".
- ^ "UniProt, Q15906" (jezik: en.). Pristupljeno 8. 12. 2021.CS1 održavanje: nepoznati jezik (link)
- ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- ^ a b "International Mouse Phenotyping Consortium".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 8. 12. 2021.
Doponska literatura
uredi- Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, Washburn MP, Conaway RC, Conaway JW (Apr 2005). "The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes". The Journal of Biological Chemistry. 280 (14): 13665–70. doi:10.1074/jbc.M500001200. PMID 15647280.
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Denoeud F, Kapranov P, Ucla C, Frankish A, Castelo R, Drenkow J, Lagarde J, Alioto T, Manzano C, Chrast J, Dike S, Wyss C, Henrichsen CN, Holroyd N, Dickson MC, Taylor R, Hance Z, Foissac S, Myers RM, Rogers J, Hubbard T, Harrow J, Guigó R, Gingeras TR, Antonarakis SE, Reymond A (Jun 2007). "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions". Genome Research. 17 (6): 746–59. doi:10.1101/gr.5660607. PMC 1891335. PMID 17567994.