SART1
U4/U6.U5 tri-snRNP-pridruženi protein 1 je protein koji je kod ljudi kodiran genom SART1.[5][6]
Ovaj gen kodira dva proteina, protein SART1 (800) eksprimiran u u jedru većine ćelija koje se razmnožavaju i protein SART1 (259) u citosolu epitelnog karcinoma. SART1 (259) protein prevodi se mehanizmom-1 pomjeranja okvira tokom posttranskripcijske regulacije. Smatra se da su dva kodirana proteina uključena u regulaciju proliferacije. Oba proteina imaju odbacivanje tumorskih antigena. Protein SART1 (259) ima tumorski epitop koji je sposoban inducirati HLA-A24 02 ograničene citotoksične T-limfocite u pacijenata s rakom. Ovaj SART1 (259) antigen može biti koristan u specifičnoj imunoterapiji za oboljele od raka i može poslužiti kao paradigmatski alat za dijagnostiku i liječenje pacijenata sa atopijom. Utvrđeno je da je protein SART1 (259) bitan za regrutiranje tri-snRNP u presplajsosomu na putunjegovog sklapanja.[6]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca je 800 aminokiselina, а molekulska težina 90.255 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MGSSKKHRGE | KEAAGTTAAA | GTGGATEQPP | RHREHKKHKH | RSGGSGGSGG | ||||
| ERRKRSRERG | GERGSGRRGA | EAEARSSTHG | RERSQAEPSE | RRVKREKRDD | ||||
| GYEAAASSKT | SSGDASSLSI | EETNKLRAKL | GLKPLEVNAI | KKEAGTKEEP | ||||
| VTADVINPMA | LRQREELREK | LAAAKEKRLL | NQKLGKIKTL | GEDDPWLDDT | ||||
| AAWIERSRQL | QKEKDLAEKR | AKLLEEMDQE | FGVSTLVEEE | FGQRRQDLYS | ||||
| ARDLQGLTVE | HAIDSFREGE | TMILTLKDKG | VLQEEEDVLV | NVNLVDKERA | ||||
| EKNVELRKKK | PDYLPYAEDE | SVDDLAQQKP | RSILSKYDEE | LEGERPHSFR | ||||
| LEQGGTADGL | RERELEEIRA | KLRLQAQSLS | TVGPRLASEY | LTPEEMVTFK | ||||
| KTKRRVKKIR | KKEKEVVVRA | DDLLPLGDQT | QDGDFGSRLR | GRGRRRVSEV | ||||
| EEEKEPVPQP | LPSDDTRVEN | MDISDEEEGG | APPPGSPQVL | EEDEAELELQ | ||||
| KQLEKGRRLR | QLQQLQQLRD | SGEKVVEIVK | KLESRQRGWE | EDEDPERKGA | ||||
| IVFNATSEFC | RTLGEIPTYG | LAGNREEQEE | LMDFERDEER | SANGGSESDG | ||||
| EENIGWSTVN | LDEEKQQQDF | SASSTTILDE | EPIVNRGLAA | ALLLCQNKGL | ||||
| LETTVQKVAR | VKAPNKSLPS | AVYCIEDKMA | IDDKYSRREE | YRGFTQDFKE | ||||
| KDGYKPDVKI | EYVDETGRKL | TPKEAFRQLS | HRFHGKGSGK | MKTERRMKKL | ||||
| DEEALLKKMS | SSDTPLGTVA | LLQEKQKAQK | TPYIVLSGSG | KSMNANTITK | ||||
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000175467 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000039148 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Shichijo S, Nakao M, Imai Y, Takasu H, Kawamoto M, Niiya F, Yang D, Toh Y, Yamana H, Itoh K (Mar 1998). "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes". J Exp Med. 187 (3): 277–88. doi:10.1084/jem.187.3.277. PMC 2212124. PMID 9449708.
- ^ a b "Entrez Gene: SART1 squamous cell carcinoma antigen recognized by T cells".
- ^ "UniProt, O43290". Pristupljeno 27. 8. 2021.
Dopunska literatura
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- Kikuchi M, Nakao M, Inoue Y, et al. (1999). "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes". Int. J. Cancer. 81 (3): 459–66. doi:10.1002/(SICI)1097-0215(19990505)81:3<459::AID-IJC21>3.0.CO;2-6. PMID 10209962.
- Matsunaga K, Nakao M, Masuoka K, et al. (1999). "Cytokines required for induction of histocompatibility leukocyte antigen-class I-restricted and tumor-specific cytotoxic T lymphocytes by a SART1-derived peptide". Jpn. J. Cancer Res. 90 (9): 1007–15. doi:10.1111/j.1349-7006.1999.tb00849.x. PMC 5926162. PMID 10551332.
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- Bolland DJ, Hewitt JE (2001). "Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis". Gene. 271 (1): 43–9. doi:10.1016/S0378-1119(01)00504-2. PMID 11410364.
- Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
- Bell M, Schreiner S, Damianov A, et al. (2002). "p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor". EMBO J. 21 (11): 2724–35. doi:10.1093/emboj/21.11.2724. PMC 126028. PMID 12032085.
- Wheatley AP, Bolland DJ, Hewitt JE, et al. (2003). "Identification of the autoantigen SART-1 as a candidate gene for the development of atopy". Hum. Mol. Genet. 11 (18): 2143–6. doi:10.1093/hmg/11.18.2143. PMID 12189166.
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- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Vertegaal AC, Ogg SC, Jaffray E, et al. (2004). "A proteomic study of SUMO-2 target proteins". J. Biol. Chem. 279 (32): 33791–8. doi:10.1074/jbc.M404201200. PMID 15175327.
- Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.