RPAIN

RPAIN
Identifikatori
Aliasi	RPAIN
Vanjski ID-jevi	OMIM: 617299 MGI: 1916973 HomoloGene: 13006 GeneCards: RPAIN
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	5,432,877 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	70,868,659 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje iona metala; • GO:0032403 protein-containing complex binding;
Ćelijska komponenta	• citoplazma; • PML body; • jedro; • fibrillar center;
Biološki proces	• DNA-dependent DNA replication; • DNA recombination; • response to UV; • GO:0100026 Popravka DNK; • protein import into nucleus;
	Izvori:Amigo / QuickGO
Ortolozi
	84268
	69723
	ENSG00000129197
	ENSMUSG00000018449
	Q86UA6
	Q9CWY9
NM_001033002; NM_001160243; NM_001160244; NM_001160246; NM_001160266;
	NM_001160267; NM_032308
	NM_001252413; NM_001252414; NM_001252415; NM_027186
	NP_001028174; NP_001153715; NP_001153716; NP_001153718; NP_001153738
	NP_001239342; NP_001239343; NP_001239344; NP_081462
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

RPA-interaktivni protein je protein koji je kod ljudi kodiran genom that in RPAIN.^[5]^[6]^[7]

Dužina polipeptidnog lanca je 219 aminokiselina, a molekulska težina 24.784^[8].

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MAESLRSPRR	SLYKLVGSPP	WKEAFRQRCL	ERMRNSRDRL	LNRYRQAGSS
GPGNSQNSFL	VQEVMEEEWN	ALQSVENCPE	DLAQLEELID	MAVLEEIQQE
LIKQEQSIIS	EYEKSLQFDE	KCLSIMLAEW	EANPLICPVC	TKYNLRITSG
VVVCQCGLSI	PSHSSELTEQ	KLRACLEGSI	NEHSAHCPHT	PEFSVTGGTE
EKSSLLMSCL	ACDTWAVIL

Kloniranje i ekspresija

Pregledom biblioteke cDNK ljudskog fetusog mozga, Chen et al. (2005) klonirali su devet prerađenih varijanti RPAIN-a, koje su nazvali RIP. Izvedeni protein sa 219 aminokiselina pune dužine ima N-terminalnu regiju baza bogatu argininom, a slijedi ga kiselo područje jedra i dva C-terminalna domena nalik na cinkov prst. U nekoliko ostalih RPAIN izoformi nedostaje jedan ili oba C-terminalna domena, slična cinkovom prstu. RPAIN je visoko konzerviran kod eukariota, s ortolozima kod pacova, miša i Xenopus laevis. PCR analiza više tkiva cDNK pokazala je ekspresiju iRNK RPAIN-a u svim testiranim ljudskim tkivima. RPAIN označen fluorescencijom in situ lokalizovan na iedrima u ćelijama HEK293.

Chen et al. (2005) utvrdili su da gen RPAIN sadrži najmanje sedam egzona.^[9]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000129197 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000018449 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Park J, Seo T, Kim H, Choe J (Sep 2005). "Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies". Mol Cell Biol. 25 (18): 8202–14. doi:10.1128/MCB.25.18.8202-8214.2005. PMC 1234305. PMID 16135809.
^ Chen JZ, Huang SD, Ji CN, Pang RY, Xie Y, Xue JL (Jul 2005). "Identification, expression pattern, and subcellular location of human RIP isoforms". DNA Cell Biol. 24 (7): 464–9. doi:10.1089/dna.2005.24.464. PMID 16008515.
^ "Entrez Gene: RPAIN RPA interacting protein".
^ "UniProt, Q86UA6". Pristupljeno 11. 6. 2021.
^ Chen, J.-Z., Huang, S.-D., Ji, C.-N., Pang, R.-Y., Xie, Y., Xue, J.-L. Identification, expression pattern, and subcellular location of human RIP isoforms. DNA Cell Biol. 24: 464-469, 2005. [PubMed]: 16008515

Dopunska literatura

Blonska M, You Y, Geleziunas R, Lin X (2004). "Restoration of NF-kappaB activation by tumor necrosis factor alpha receptor complex-targeted MEKK3 in receptor-interacting protein-deficient cells". Mol. Cell. Biol. 24 (24): 10757–65. doi:10.1128/MCB.24.24.10757-10765.2004. PMC 533972. PMID 15572679.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000129197 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000018449 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid16135809-5] Park J, Seo T, Kim H, Choe J (Sep 2005). "Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies". Mol Cell Biol. 25 (18): 8202–14. doi:10.1128/MCB.25.18.8202-8214.2005. PMC 1234305. PMID 16135809.

[pmid16008515-6] Chen JZ, Huang SD, Ji CN, Pang RY, Xie Y, Xue JL (Jul 2005). "Identification, expression pattern, and subcellular location of human RIP isoforms". DNA Cell Biol. 24 (7): 464–9. doi:10.1089/dna.2005.24.464. PMID 16008515.

[entrez-7] "Entrez Gene: RPAIN RPA interacting protein".

[UniProt-8] "UniProt, Q86UA6". Pristupljeno 11. 6. 2021.

[9] Chen, J.-Z., Huang, S.-D., Ji, C.-N., Pang, R.-Y., Xie, Y., Xue, J.-L. Identification, expression pattern, and subcellular location of human RIP isoforms. DNA Cell Biol. 24: 464-469, 2005. [PubMed]: 16008515

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