RPAIN
RPA-interaktivni protein je protein koji je kod ljudi kodiran genom that in RPAIN.[5][6][7]
Dužina polipeptidnog lanca je 219 aminokiselina, a molekulska težina 24.784[8].
Aminokiselinska sekvenca
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAESLRSPRR | SLYKLVGSPP | WKEAFRQRCL | ERMRNSRDRL | LNRYRQAGSS | ||||
| GPGNSQNSFL | VQEVMEEEWN | ALQSVENCPE | DLAQLEELID | MAVLEEIQQE | ||||
| LIKQEQSIIS | EYEKSLQFDE | KCLSIMLAEW | EANPLICPVC | TKYNLRITSG | ||||
| VVVCQCGLSI | PSHSSELTEQ | KLRACLEGSI | NEHSAHCPHT | PEFSVTGGTE | ||||
| EKSSLLMSCL | ACDTWAVIL |
Kloniranje i ekspresija uredi
Pregledom biblioteke cDNK ljudskog fetusog mozga, Chen et al. (2005) klonirali su devet prerađenih varijanti RPAIN-a, koje su nazvali RIP. Izvedeni protein sa 219 aminokiselina pune dužine ima N-terminalnu regiju baza bogatu argininom, a slijedi ga kiselo područje jedra i dva C-terminalna domena nalik na cinkov prst. U nekoliko ostalih RPAIN izoformi nedostaje jedan ili oba C-terminalna domena, slična cinkovom prstu. RPAIN je visoko konzerviran kod eukariota, s ortolozima kod pacova, miša i Xenopus laevis. PCR analiza više tkiva cDNK pokazala je ekspresiju iRNK RPAIN-a u svim testiranim ljudskim tkivima. RPAIN označen fluorescencijom in situ lokalizovan na iedrima u ćelijama HEK293.
Chen et al. (2005) utvrdili su da gen RPAIN sadrži najmanje sedam egzona.[9]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000129197 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000018449 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Park J, Seo T, Kim H, Choe J (Sep 2005). "Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies". Mol Cell Biol. 25 (18): 8202–14. doi:10.1128/MCB.25.18.8202-8214.2005. PMC 1234305. PMID 16135809.
- ^ Chen JZ, Huang SD, Ji CN, Pang RY, Xie Y, Xue JL (Jul 2005). "Identification, expression pattern, and subcellular location of human RIP isoforms". DNA Cell Biol. 24 (7): 464–9. doi:10.1089/dna.2005.24.464. PMID 16008515.
- ^ "Entrez Gene: RPAIN RPA interacting protein".
- ^ "UniProt, Q86UA6". Pristupljeno 11. 6. 2021.
- ^ Chen, J.-Z., Huang, S.-D., Ji, C.-N., Pang, R.-Y., Xie, Y., Xue, J.-L. Identification, expression pattern, and subcellular location of human RIP isoforms. DNA Cell Biol. 24: 464-469, 2005. [PubMed]: 16008515
Dopunska literatura uredi
- Blonska M, You Y, Geleziunas R, Lin X (2004). "Restoration of NF-kappaB activation by tumor necrosis factor alpha receptor complex-targeted MEKK3 in receptor-interacting protein-deficient cells". Mol. Cell. Biol. 24 (24): 10757–65. doi:10.1128/MCB.24.24.10757-10765.2004. PMC 533972. PMID 15572679.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.