PLOD3
Prokolagen-lizin,2-oksoglutarat 5-dioksigenaza 3 je enzim koji je kod ljudi kodiran genom PLOD3.[5][6][7]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca je 738 aminokiselina, а molekulska težina 84.785 Da.[8]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MTSSGPGPRF | LLLLPLLLPP | AASASDRPRG | RDPVNPEKLL | VITVATAETE | ||||
| GYLRFLRSAE | FFNYTVRTLG | LGEEWRGGDV | ARTVGGGQKV | RWLKKEMEKY | ||||
| ADREDMIIMF | VDSYDVILAG | SPTELLKKFV | QSGSRLLFSA | ESFCWPEWGL | ||||
| AEQYPEVGTG | KRFLNSGGFI | GFATTIHQIV | RQWKYKDDDD | DQLFYTRLYL | ||||
| DPGLREKLSL | NLDHKSRIFQ | NLNGALDEVV | LKFDRNRVRI | RNVAYDTLPI | ||||
| VVHGNGPTKL | QLNYLGNYVP | NGWTPEGGCG | FCNQDRRTLP | GGQPPPRVFL | ||||
| AVFVEQPTPF | LPRFLQRLLL | LDYPPDRVTL | FLHNNEVFHE | PHIADSWPQL | ||||
| QDHFSAVKLV | GPEEALSPGE | ARDMAMDLCR | QDPECEFYFS | LDADAVLTNL | ||||
| QTLRILIEEN | RKVIAPMLSR | HGKLWSNFWG | ALSPDEYYAR | SEDYVELVQR | ||||
| KRVGVWNVPY | ISQAYVIRGD | TLRMELPQRD | VFSGSDTDPD | MAFCKSFRDK | ||||
| GIFLHLSNQH | EFGRLLATSR | YDTEHLHPDL | WQIFDNPVDW | KEQYIHENYS | ||||
| RALEGEGIVE | QPCPDVYWFP | LLSEQMCDEL | VAEMEHYGQW | SGGRHEDSRL | ||||
| AGGYENVPTV | DIHMKQVGYE | DQWLQLLRTY | VGPMTESLFP | GYHTKARAVM | ||||
| NFVVRYRPDE | QPSLRPHHDS | STFTLNVALN | HKGLDYEGGG | CRFLRYDCVI | ||||
| SSPRKGWALL | HPGRLTHYHE | GLPTTWGTRY | IMVSFVDP |
Funkcija
urediProtein kodiran ovim genom je enzim vezan za membranu, homodimerni enzim koji je lokaliziran u cisternama grubog hrapavog endoplazmatskog retikuluma. Enzim (kofaktori gvožđe i askorbat) katalizira hidroksilaciju ostataka lizina u kolagenim peptidima. Rezultirajuće hidroksilzil grupe su mjesta vezivanja ugljikohidrata u kolagenu i stoga su kritične za stabilnost međumolekulskih umrežavanja. Neki pacijenti sa Ehlers-Danlosovim sindromom tip VIB imaju nedostatke u aktivnosti lizil-hidroksilaze.[7]
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000106397 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000004846 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Passoja K, Rautavuoma K, Ala-Kokko L, Kosonen T, Kivirikko KI (Sep 1998). "Cloning and characterization of a third human lysyl hydroxylase isoform". Proc Natl Acad Sci U S A. 95 (18): 10482–10486. doi:10.1073/pnas.95.18.10482. PMC 27920. PMID 9724729.
- ^ Valtavaara M, Szpirer C, Szpirer J, Myllyla R (Jun 1998). "Primary structure, tissue distribution, and chromosomal localization of a novel isoform of lysyl hydroxylase (lysyl hydroxylase 3)". J Biol Chem. 273 (21): 12881–12886. doi:10.1074/jbc.273.21.12881. PMID 9582318.
- ^ a b "Entrez Gene: PLOD3 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3".
- ^ "UniProt, O60568" (jezik: engleski). Pristupljeno 28. 9. 2021.
Dopunska literatura
uredi- Salo AM, Wang C, Sipilä L, et al. (2006). "Lysyl hydroxylase 3 (LH3) modifies proteins in the extracellular space, a novel mechanism for matrix remodeling". J. Cell. Physiol. 207 (3): 644–653. doi:10.1002/jcp.20596. PMID 16447251. S2CID 19594208.
- Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–126. doi:10.1093/dnares/12.2.117. PMID 16303743.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–164. doi:10.1038/nature01782. PMID 12853948.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Wang C, Luosujärvi H, Heikkinen J, et al. (2003). "The third activity for lysyl hydroxylase 3: galactosylation of hydroxylysyl residues in collagens in vitro". Matrix Biol. 21 (7): 559–566. doi:10.1016/S0945-053X(02)00071-9. PMID 12475640.
- Rautavuoma K, Takaluoma K, Passoja K, et al. (2002). "Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1–3. The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity". J. Biol. Chem. 277 (25): 23084–23091. doi:10.1074/jbc.M112077200. PMID 11956192.
- Ruotsalainen H, Vanhatupa S, Tampio M, et al. (2001). "Complete genomic structure of mouse lysyl hydroxylase 2 and lysyl hydroxylase 3/collagen glucosyltransferase". Matrix Biol. 20 (2): 137–146. doi:10.1016/S0945-053X(01)00130-5. PMID 11334715.
- Heikkinen J, Risteli M, Wang C, et al. (2000). "Lysyl hydroxylase 3 is a multifunctional protein possessing collagen glucosyltransferase activity". J. Biol. Chem. 275 (46): 36158–36163. doi:10.1074/jbc.M006203200. PMID 10934207.
- Rautavuoma K, Passoja K, Helaakoski T, Kivirikko KI (2000). "Complete exon-intron organization of the gene for human lysyl hydroxylase 3 (LH3)". Matrix Biol. 19 (1): 73–79. doi:10.1016/S0945-053X(99)00058-X. PMID 10686427.