LRRC23

LRRC23
Identifikatori
Aliasi	LRRC23
Vanjski ID-jevi	MGI: 1315192 HomoloGene: 5082 GeneCards: LRRC23
Lokacija gena (čovjek)
Hrom.	Hromosom 12 (čovjek)
Kraj	6,914,241 bp
Lokacija gena (miš)
Hrom.	Hromosom 6 (miš)
Kraj	124,756,690 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• molekularna funkcija;
Ćelijska komponenta	• ćelijska komponenta; • citoplazma; • citosol;
Biološki proces	• GO:0022610 biološki proces;
	Izvori:Amigo / QuickGO
Ortolozi
	10233
	16977
	ENSG00000010626
	ENSMUSG00000030125
	Q53EV4
	O35125
	NM_201650; NM_001135217; NM_006992; NM_181613
	NM_013588; NM_001302555
	NP_001128689; NP_008923; NP_964013
	NP_001289484
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Leucinski bogati protein 23 sa ponavljanjem je protein koji je kod ljudi kodiran genom LRRC23.^[5]^[6]^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 343 aminokiseline, a molekulska težina 39.761 Da.^[8]

10	20	30	40	50
MSDEDDLEDS	EPDQDDSEKE	EDEKETEEGE	DYRKEGEEFP	EEWLPTPLTE
DMMKEGLSLL	CKTGNGLAHA	YVKLEVKERD	LTDIYLLRSY	IHLRYVDISE
NHLTDLSPLN	YLTHLLWLKA	DGNRLRSAQM	NELPYLQIAS	FAYNQITDTE
GISHPRLETL	NLKGNSIHMV	TGLDPEKLIS	LHTVELRGNQ	LESTLGINLP
KLKNLYLAQN	MLKKVEGLED	LSNLTTLHLR	DNQIDTLSGF	SREMKSLQYL
NLRGNMVANL	GELAKLRDLP	KLRALVLLDN	PCTDETSYRQ	EALVQMPYLE
RLDKEFYEEE	ERAEADVIRQ	RLKEEKEQEP	EPQRDLEPEQ	SLI

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Protein

Trodimenzijska struktura LRRC23, prema BLAST-u

Iako je stvarna struktura LRRC23 nepoznata, usporedba sa kristalnim strukturama različitih sličnih proteina, poput 2OMW A (e-vrijednost 1,00e-17) otkriva strukturu tipsku za druge proteine bogate leucinom. Naizmjenični beta-listovi i zavojnice stvaraju spiralni peptidni lanac koji tvori lučni oblik s beta-listovima koji zauzimaju konkavnu površinu.^[9]

Poravnata struktura 2OMW_A s LRRC23 obuhvata aminokiseline 72-272 proteina LRRC23. Konzervirane sekvence označeni su žutom bojom, pokazujući pravilnost razmaka i unutar ponovljajuće strukture. Ovaj model je nastao korišćenjem softvera Cn3D koji je obezbedio Nacionalni centar za biotehnološke informacije (NCBI).

Funkcija

Funkcija LRRC23 je nepoznata. Član je proteinskih ponavljanja bogatog leucinom, koji su poznati po učešću u interakcijama protein-protein. Eksperimentalni dokazi ukazuju na to da LRRC23 stupa u interakciju s proteinom CD28, na putu koji je povezan sa imunskim sistemom i razvojem regulatornih T-ćelija koje kontroliraju spontanu autoimunsku bolest.^[10]

Ljudski genom proizvodi tri poznate varijante LRRC23.^[7] Najveća prerađena varijanta, varijanta 3, sadrži osam egzona. Varijante 1 i 2 koriste alternativne prve egzone, a varijanta 2 isključuje sedmi egzon, dajući mu ukupno sedam egzona koji čine iRNK.

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000010626 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000030125 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tzachanis D, Berezovskaya A, Nadler LM, Boussiotis VA (Feb 2002). "Blockade of B7/CD28 in mixed lymphocyte reaction cultures results in the generation of alternatively activated macrophages, which suppress T-cell responses". Blood. 99 (4): 1465–73. doi:10.1182/blood.V99.4.1465. PMID 11830501.
^ Chang TT, Kuchroo VK, Sharpe AH (Feb 2002). "Role of the B7-CD28/CTLA-4 pathway in autoimmune disease". Signal Transduction Pathways in Autoimmunity. Current Directions in Autoimmunity. 5. str. 113–30. doi:10.1159/000060550. ISBN 3-8055-7308-1. PMID 11826754.
^ ^a ^b "Entrez Gene: LRRC23 leucine rich repeat containing 23".
^ "UniProt, Q53EV4". Pristupljeno 11. 8. 2021.
^ NCBI Structure CBlast https://www.ncbi.nlm.nih.gov/Structure/cblast/cblast.cgi?client=entrez&query_gi=206725447&hit=149242643&hsp=1&output=html&pagenum=1&epp=20&sort=evalue&view=graphic&subset=allmmdb
^ Salomon B, Lenschow DJ, Rhee L, Ashourian N, Singh B, Sharpe A, Bluestone JA (Apr 2000). "B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune diabetes". Immunity. 12 (4): 431–40. doi:10.1016/S1074-7613(00)80195-8. PMID 10795741.

Dopunska literatura

Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Ansari-Lari MA, Muzny DM, Lu J, Lu F, Lilley CE, Spanos S, Malley T, Gibbs RA (Apr 1996). "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13". Genome Research. 6 (4): 314–26. doi:10.1101/gr.6.4.314. PMID 8723724.
Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Ansari-Lari MA, Shen Y, Muzny DM, Lee W, Gibbs RA (Mar 1997). "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination". Genome Research. 7 (3): 268–80. doi:10.1101/gr.7.3.268. PMID 9074930.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Tasheva ES, An K, Boyle DL, Conrad GW (2006). "Expression and localization of leucine-rich B7 protein in human ocular tissues". Molecular Vision. 11: 452–60. PMID 16030496.

Vanjski linkovi

LRRC23 lokacija ljudskog genoma UCSC Genome Browser.

LRRC23 human gene details in the UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000010626 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000030125 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid11830501-5] Tzachanis D, Berezovskaya A, Nadler LM, Boussiotis VA (Feb 2002). "Blockade of B7/CD28 in mixed lymphocyte reaction cultures results in the generation of alternatively activated macrophages, which suppress T-cell responses". Blood. 99 (4): 1465–73. doi:10.1182/blood.V99.4.1465. PMID 11830501.

[pmid11826754-6] Chang TT, Kuchroo VK, Sharpe AH (Feb 2002). "Role of the B7-CD28/CTLA-4 pathway in autoimmune disease". Signal Transduction Pathways in Autoimmunity. Current Directions in Autoimmunity. 5. str. 113–30. doi:10.1159/000060550. ISBN 3-8055-7308-1. PMID 11826754.

[entrez-7] "Entrez Gene: LRRC23 leucine rich repeat containing 23".

[UniProt-8] "UniProt, Q53EV4". Pristupljeno 11. 8. 2021.

[9] NCBI Structure CBlast https://www.ncbi.nlm.nih.gov/Structure/cblast/cblast.cgi?client=entrez&query_gi=206725447&hit=149242643&hsp=1&output=html&pagenum=1&epp=20&sort=evalue&view=graphic&subset=allmmdb

[10] Salomon B, Lenschow DJ, Rhee L, Ashourian N, Singh B, Sharpe A, Bluestone JA (Apr 2000). "B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune diabetes". Immunity. 12 (4): 431–40. doi:10.1016/S1074-7613(00)80195-8. PMID 10795741.

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