GPN3

GPN3
Identifikatori
Aliasi	GPN3
Vanjski ID-jevi	MGI: 1289326 HomoloGene: 6487 GeneCards: GPN3
Lokacija gena (čovjek)
Hrom.	Hromosom 12 (čovjek)
Kraj	110,469,268 bp
Lokacija gena (miš)
Hrom.	Hromosom 5 (miš)
Kraj	122,520,965 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ortolozi
	51184
	68080
	ENSG00000111231
	ENSMUSG00000029464
	Q9UHW5
	Q9D3W4
	NM_001164372; NM_001164373; NM_016301
	NM_024216
	NP_001157844; NP_001157845; NP_057385
	NP_077178
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

GPN-petlja GTPaze 3 je enzim koji je kod ljudi kodiran genom GPN3.^[5]^[6]

Maruyama i Sugano razvili su metod zamjene kape strukture iRNK oligoribonukleotidom (r-oligo) za označavanje 5' kraja eukariotskih iRNK. Sastoji se u uklanjanju poklopca pirofosfatazom nikotinske kiseline (TAP) i vezanjem r-oligonukleotid na dekapirane iRNK s T4 RNK-ligazom. Ova reakcija učinjena je specifičnom za kapu alkalnom fosfatazom, uklanjanjem 5'-fosfata nekapiranih RNK prije tretmana TAP. Za razliku od uobičajenih metoda koje označavaju 5 'kraj cDNK, ovaj metod posebno obilježava kapirani kraj iRNK sintetskim r-oligo prije sinteze prvog lanca cDNK. 5 'kraj iRNK je jednostavno identificiran lančanom reakcijom reverzne transkripcijske-polimeraze (RT-PCR).^[7]

Aminokiselinska sekvenca uredi

Dužina polipeptidnog lanca je 284 aminokiseline, a molekulska težina 32.761 Da.^[8].

10	20	30	40	50
MPRYAQLVMG	PAGSGKSTYC	ATMVQHCEAL	NRSVQVVNLD	PAAEHFNYSV
MADIRELIEV	DDVMEDDSLR	FGPNGGLVFC	MEYFANNFDW	LENCLGHVED
DYILFDCPGQ	IELYTHLPVM	KQLVQQLEQW	EFRVCGVFLV	DSQFMVESFK
FISGILAALS	AMISLEIPQV	NIMTKMDLLS	KKAKKEIEKF	LDPDMYSLLE
DSTSDLRSKK	FKKLTKAICG	LIDDYSMVRF	LPYDQSDEES	MNIVLQHIDF
AIQYGEDLEF	KEPKEREDES	SSMFDEYFQE	CQDE

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Reference uredi

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000111231 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000029464 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A (Oct 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
^ "Entrez Gene: ATPBD1C ATP binding domain 1 family, member C".
^ Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
^ "UniProt, Q9UHW5". Pristupljeno 10. 8. 2021.

Dopunska literatura uredi

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000111231 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000029464 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12975309-5] Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A (Oct 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.

[entrez-6] "Entrez Gene: ATPBD1C ATP binding domain 1 family, member C".

[7] Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.

[UniProt-8] "UniProt, Q9UHW5". Pristupljeno 10. 8. 2021.

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