FCF1
Homolog FCF1 proteina prerade rRNK je protein koji je kod ljudi kodiran genom FCF1.
Neokarakterizirani protrini Fcf1 i Fcf2 Saccharomyces cerevisiae, kodirani otvorenim okvirom čitanja YDR339c, odnosno YLR051c, identificirani su u eksperimentu za tandemsko prečišćavanje afiniteta poznatog 40S faktora Faf1p. Većina proteina povezanih s TAP-Faf1p su transaktivni faktori koji su uključeni u preradu pre-rRNK i biogenezu podjedinice 40S, u skladu s prethodno uočenom ulogom Faf1p u sintezi 18S rRNA. Fcf1p i Fcf2p su neophodni i lokaliziraju se u jedarcetu. Iscrpljivanje Fcf1p i Fcf2p dovodi do smanjenja sinteze 18S rRNK, što rezultira deficitom u ribosomskim podjedinicama 40S. Northern blot analiza ukazuje na neefikasnu obradu pre-rRNK na mjestima cijepanja A (0), A (1) i A (2).[5][6]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca je 198 aminokiselina, a molekulska težina 23 370 Da .[7].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MGKQKKTRKY | ATMKRMLSLR | DQRLKEKDRL | KPKKKEKKDP | SALKEREVPQ | ||||
| HPSCLFFQYN | TQLGPPYHIL | VDTNFINFSI | KAKLDLVQSM | MDCLYAKCIP | ||||
| CITDCVMAEI | EKLGQKYRVA | LRIAKDPRFE | RLPCTHKGTY | ADDCLVQRVT | ||||
| QHKCYIVATV | DRDLKRRIRK | IPGVPIMYIS | NHRYNIERMP | DDYGAPRF |
- Simboli
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000119616 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000021243 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Rempola B, Karkusiewicz I, Piekarska I, Rytka J (Jun 2006). "Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing". Biochem Biophys Res Commun. 346 (2): 546–54. doi:10.1016/j.bbrc.2006.05.140. PMID 16762320.
- ^ "Entrez Gene: FCF1 FCF1 small subunit (SSU) processome component homolog (S. cerevisiae)".
- ^ "UniProt, Q9Y324". Pristupljeno 25. 7. 2021.
Dopunska literatura
uredi- Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
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- Heilig R, Eckenberg R, Petit JL, et al. (2003). "The DNA sequence and analysis of human chromosome 14". Nature. 421 (6923): 601–7. doi:10.1038/nature01348. PMID 12508121.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Lai CH, Chou CY, Ch'ang LY, et al. (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.