ENTPD2

Ektonukleozid trifosfat-difosfohidrolaza 2 je enzim koji je kod ljudi kodiran genom ENTPD2.

ENTPD2
Identifikatori
Aliasi	ENTPD2
Vanjski ID-jevi	OMIM: 602012 MGI: 1096863 HomoloGene: 20333 GeneCards: ENTPD2
Lokacija gena (čovjek) Hrom. Hromosom 9 (čovjek) Bend 9q34.3 Početak 137,048,107 bp Kraj 137,054,061 bp
Lokacija gena (miš) Hrom. Hromosom 2 (miš) Bend 2|2 A3 Početak 25,285,886 bp Kraj 25,291,333 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • nucleotide binding • nucleoside-diphosphatase activity • hydrolase activity • ATP binding • nucleoside-triphosphatase activity Ćelijska komponenta • integral component of membrane • endoplasmic reticulum membrane • membrana • Endoplazmatski retikulum • Egzosom • ćelijska membrana • basement membrane Biološki proces • G protein-coupled receptor signaling pathway • purine ribonucleoside diphosphate catabolic process • platelet activation • nucleobase-containing small molecule catabolic process Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	954	12496
Ensembl	ENSG00000054179	ENSMUSG00000015085
UniProt	Q9Y5L3	O55026
RefSeq (mRNK)	NM_001246 NM_203468	NM_009849
RefSeq (bjelančevina)	NP_001237 NP_982293	NP_033979
Lokacija (UCSC)	Chr 9: 137.05 – 137.05 Mb	Chr 2: 25.29 – 25.29 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 495 aminokiselina, а molekulska težina 53.665 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MAGKVRSLLP		PLLLAAAGLA		GLLLLCVPTR		DVREPPALKY		GIVLDAGSSH
TSMFIYKWPA		DKENDTGIVG		QHSSCDVPGG		GISSYADNPS		GASQSLVGCL
EQALQDVPKE		RHAGTPLYLG		ATAGMRLLNL		TNPEASTSVL		MAVTHTLTQY
PFDFRGARIL		SGQEEGVFGW		VTANYLLENF		IKYGWVGRWF		RPRKGTLGAM
DLGGASTQIT		FETTSPAEDR		ASEVQLHLYG		QHYRVYTHSF		LCYGRDQVLQ
RLLASALQTH		GFHPCWPRGF		STQVLLGDVY		QSPCTMAQRP		QNFNSSARVS
LSGSSDPHLC		RDLVSGLFSF		SSCPFSRCSF		NGVFQPPVAG		NFVAFSAFFY
TVDFLRTSMG		LPVATLQQLE		AAAVNVCNQT		WAQLQARVPG		QRARLADYCA
GAMFVQQLLS		RGYGFDERAF		GGVIFQKKAA		DTAVGWALGY		MLNLTNLIPA
DPPGLRKGTD		FSSWVVLLLL		FASALLAALV		LLLRQVHSAK		LPSTI

Funkcija

Protein kodiran ovim genom je enzim tipa 2, iz porodice ekto-nukleozid trifosfat difosfohidrolaza (E-NTPDaza). E-NTPDaze su porodica ekto-nukleozidaza koje hidroliziraju 5'-trifosfate. Ova ekto-ATPaza je integralni membranski protein. Alternativna prerada ovog gena dovodi do više varijanti transkripta.

Naučnici sa Univerziteta u Warwicku pokazali su da E-NTPDaza 2 stimulira rast ljudskog oka: tretiranjem enzima na punoglavcima, otkriveno je da dobijaju dodatne oči na svom tijelu.

Dopunska literatura

• Javed R, Yarimizu K, Pelletier N, et al. (2007). "Mutagenesis of lysine 62, asparagine 64, and conserved region 1 reduces the activity of human ecto-ATPase (NTPDase 2)". Biochemistry. 46 (22): 6617–27. doi:10.1021/bi700036e. PMID 17489562.
• Mukasa T, Lee Y, Knowles AF (2005). "Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8)". Biochemistry. 44 (33): 11160–70. doi:10.1021/bi050019k. PMID 16101300.
• Jhandier MN, Kruglov EA, Lavoie EG, et al. (2005). "Portal fibroblasts regulate the proliferation of bile duct epithelia via expression of NTPDase2". J. Biol. Chem. 280 (24): 22986–92. doi:10.1074/jbc.M412371200. PMID 15799977.
• Dranoff JA, Kruglov EA, Toure J, et al. (2005). "Ectonucleotidase NTPDase2 is selectively down-regulated in biliary cirrhosis". J. Investig. Med. 52 (7): 475–82. doi:10.2310/6650.2004.00710. PMID 15651265.
• Alvarado-Castillo C, Harden TK, Boyer JL (2005). "Regulation of P2Y1 receptor-mediated signaling by the ectonucleoside triphosphate diphosphohydrolase isozymes NTPDase1 and NTPDase2". Mol. Pharmacol. 67 (1): 114–22. doi:10.1124/mol.104.006908. PMID 15496502. S2CID 27764870.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Knowles AF, Chiang WC (2003). "Enzymatic and transcriptional regulation of human ecto-ATPase/E-NTPDase 2". Arch. Biochem. Biophys. 418 (2): 217–27. doi:10.1016/j.abb.2003.08.007. PMID 14522593.
• Mateo J, Kreda S, Henry CE, et al. (2003). "Requirement of Cys399 for processing of the human ecto-ATPase (NTPDase2) and its implications for determination of the activities of splice variants of the enzyme". J. Biol. Chem. 278 (41): 39960–8. doi:10.1074/jbc.M307854200. PMID 12888562.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Grinthal A, Guidotti G (2002). "Transmembrane domains confer different substrate specificities and adenosine diphosphate hydrolysis mechanisms on CD39, CD39L1, and chimeras". Biochemistry. 41 (6): 1947–56. doi:10.1021/bi015563h. PMID 11827541.
• Suzuki H, Fukunishi Y, Kagawa I, et al. (2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Mateo J, Harden TK, Boyer JL (2000). "Functional expression of a cDNA encoding a human ecto-ATPase". Br. J. Pharmacol. 128 (2): 396–402. doi:10.1038/sj.bjp.0702805. PMC 1571647. PMID 10510450.