CLIC4

CLIC4
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2AHE, 2D2Z, 3OQS
Identifikatori
Aliasi	CLIC4
Vanjski ID-jevi	OMIM: 606536 MGI: 1352754 HomoloGene: 8490 GeneCards: CLIC4
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	24,844,321 bp
Lokacija gena (miš)
Hrom.	Hromosom 4 (miš)
Kraj	135,000,125 bp
Obrazac RNK ekspresije
	; ; ; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• voltage-gated ion channel activity; • GO:0001948, GO:0016582 vezivanje za proteine; • glutathione transferase activity; • chloride channel activity;
Ćelijska komponenta	• citoplazma; • integral component of membrane; • citosol; • centrosom; • membrana; • cell-cell junction; • nuclear matrix; • microtubule cytoskeleton; • ćelijska membrana; • apical part of cell; • intracellular anatomical structure; • chloride channel complex; • centar organizacije mikrotubula; • microvillus; • cell surface; • međućelijske veze; • midbody; • mitohondrija; • actin cytoskeleton; • perinuklearno područje citoplazme; • Egzosom; • citoskelet; • cytoplasmic vesicle membrane; • GO:0016023 citoplazmatska vezikula; • jedro;
Biološki proces	• cellular response to calcium ion; • Ćelijska diferencijacija; • multicellular organism growth; • regulation of ion transmembrane transport; • endothelial cell morphogenesis; • ion transport; • keratinocyte differentiation; • vacuolar acidification; • negative regulation of cell migration; • oplodnja; • establishment or maintenance of apical/basal cell polarity; • chloride transport; • branching morphogenesis of an epithelial tube; • retina vasculature morphogenesis in camera-type eye; • regulation of cytoskeleton organization; • Angiogeneza; • glutathione metabolic process; • chloride transmembrane transport; • GO:0015915 transport; • ion transmembrane transport;
	Izvori:Amigo / QuickGO
Ortolozi
	25932
	29876
	ENSG00000169504
	ENSMUSG00000037242
	Q9Y696
	Q9QYB1
	NM_013943
	NM_013885
	NP_039234
	NP_038913
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Unutarćelijski hlorni kanal 4, znan i kao CLIC4, jest eukariotski gen koji se kod ljudi nalazi na hromosomu 1.^[5]

Amiokiselininska sekvenca uredi

Dužina polipeptidnog lanca je 253 aminokiseline, a molekulska težina 28.772 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MALSMPLNGL	KEEDKEPLIE	LFVKAGSDGE	SIGNCPFSQR	LFMILWLKGV
VFSVTTVDLK	RKPADLQNLA	PGTHPPFITF	NSEVKTDVNK	IEEFLEEVLC
PPKYLKLSPK	HPESNTAGMD	IFAKFSAYIK	NSRPEANEAL	ERGLLKTLQK
LDEYLNSPLP	DEIDENSMED	IKFSTRKFLD	GNEMTLADCN	LLPKLHIVKV
VAKKYRNFDI	PKEMTGIWRY	LTNAYSRDEF	TNTCPSDKEV	EIAYSDVAKR
LTK

Funkcija uredi

Hlorni kanali su raznolika grupa proteina koji regulišu fundamentalne ćelijske procese, uključujući stabilizaciju potencijala ćelijske membrane, transepitelni transport, održavanje unutarćelijskog pH i regulaciju volumena ćelije. Protein klornog unutarćelijskog kanala 4 (CLIC4), kodiran genom CLIC4, član je porodice p64; gen je eksprimiran u mnogim tkivima i pokazuje unutarćelijski vezikulski obrazac u PANC-1 ćelijama (ćelije raka gušterače).^[5]

Vezujući partneri uredi

CLIC4 se vezuje za dinamin I, α-tubulin, β-aktin, kreatin-kinazu i dvije 14-3-3 izoforme.^[7]

Također pogledajte uredi

Hlorni kanal

Reference uredi

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000169504 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000037242 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b "Entrez Gene: CLIC4 chloride intracellular channel 4".
^ "UniProt, Q9Y696" (jezik: engleski). Pristupljeno 23. 11. 2021.
^ Suginta W, Karoulias N, Aitken A, Ashley RH (oktobar 2001). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.

Dopunska literatura uredi

Berryman M, Bretscher A (2000). "Identification of a Novel Member of the Chloride Intracellular Channel Gene Family (CLIC5) That Associates with the Actin Cytoskeleton of Placental Microvilli". Mol. Biol. Cell. 11 (5): 1509–21. doi:10.1091/mbc.11.5.1509. PMC 14863. PMID 10793131.
Berryman MA, Goldenring JR (2004). "CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells". Cell Motil. Cytoskeleton. 56 (3): 159–72. doi:10.1002/cm.10141. PMID 14569596.
Bohman S, Matsumoto T, Suh K, et al. (2006). "Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis". J. Biol. Chem. 280 (51): 42397–404. doi:10.1074/jbc.M506724200. PMID 16239224.
Chuang JZ, Milner TA, Zhu M, Sung CH (1999). "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons". J. Neurosci. 19 (8): 2919–28. doi:10.1523/JNEUROSCI.19-08-02919.1999. PMC 6782274. PMID 10191309.
Duncan RR, Westwood PK, Boyd A, Ashley RH (1997). "Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum". J. Biol. Chem. 272 (38): 23880–6. doi:10.1074/jbc.272.38.23880. PMID 9295337.
Edwards JC (1999). "A novel p64-related Cl⁻ channel: subcellular distribution and nephron segment-specific expression". Am. J. Physiol. 276 (3 Pt 2): F398–408. doi:10.1152/ajprenal.1999.276.3.F398. PMID 10070163.
Qian Z, Okuhara D, Abe MK, Rosner MR (1999). "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel". J. Biol. Chem. 274 (3): 1621–7. doi:10.1074/jbc.274.3.1621. PMID 9880541.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW (2002). "Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-β1-Mediated Conversion of Fibroblasts to Myofibroblasts". Am. J. Pathol. 161 (2): 471–80. doi:10.1016/S0002-9440(10)64203-4. PMC 1850723. PMID 12163372.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Shiio Y, Suh KS, Lee H, et al. (2006). "Quantitative proteomic analysis of myc-induced apoptosis: a direct role for Myc induction of the mitochondrial chloride ion channel, mtCLIC/CLIC4". J. Biol. Chem. 281 (5): 2750–6. doi:10.1074/jbc.M509349200. PMID 16316993.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Singh H, Ashley RH (2007). "CLIC4 (p64H1) and its putative transmembrane domain form poorly selective, redox-regulated ion channels". Mol. Membr. Biol. 24 (1): 41–52. doi:10.1080/09687860600927907. PMID 17453412. S2CID 9986497.
Singh H, Cousin MA, Ashley RH (2007). "Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin". FEBS J. 274 (24): 6306–6316. doi:10.1111/j.1742-4658.2007.06145.x. PMID 18028448. S2CID 22494250.
Singh H (2010). "Two decades with dimorphic Chloride Intracellular Channels (CLICs)". FEBS Letters. 584 (10): 2112–21. doi:10.1016/j.febslet.2010.03.013. PMID 20226783.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Suginta W, Karoulias N, Aitken A, Ashley RH (2003). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt 1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.
Valenzuela SM, Martin DK, Por SB, et al. (1997). "Molecular cloning and expression of a chloride ion channel of cell nuclei". J. Biol. Chem. 272 (19): 12575–82. doi:10.1074/jbc.272.19.12575. PMID 9139710.
Wick M, Bürger C, Brüsselbach S, et al. (1994). "Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression". J. Cell Sci. 107 (1): 227–39. doi:10.1242/jcs.107.1.227. PMID 8175911.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.

Vanjski linkovi uredi

CLIC4 protein, human na US National Library of Medicine Medical Subject Headings (MeSH)
Lokacija ljudskog genoma CLIC4 i stranica sa detaljima o genu CLIC4 u UCSC Genome Browseru.

Ovaj članak uključuje tekst iz Nacionalne medicinske biblioteke Sjedinjenih Država, koji je u javnom vlasništvu.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000169504 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000037242 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: CLIC4 chloride intracellular channel 4".

[UniProt-6] "UniProt, Q9Y696" (jezik: engleski). Pristupljeno 23. 11. 2021.

[pmid11563969-7] Suginta W, Karoulias N, Aitken A, Ashley RH (oktobar 2001). "Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms". Biochem. J. 359 (Pt1): 55–64. doi:10.1042/0264-6021:3590055. PMC 1222121. PMID 11563969.

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