B3GALT5

B3GALT5
Identifikatori
Aliasi	B3GALT5
Vanjski ID-jevi	OMIM: 604066 MGI: 2136878 HomoloGene: 13230 GeneCards: B3GALT5
Lokacija gena (čovjek)
Hrom.	Hromosom 21 (čovjek)
Kraj	39,673,137 bp
Lokacija gena (miš)
Hrom.	Hromosom 16 (miš)
Kraj	96,121,059 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• transferase activity; • UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity; • galactosyltransferase activity; • glycosyltransferase activity; • acetylgalactosaminyltransferase activity;
Ćelijska komponenta	• integral component of membrane; • Golđijeva membrana; • Golđijev aparat; • Endoplazmatski retikulum; • membrana;
Biološki proces	• GO:0033578, GO:0033577, GO:0033575, GO:0033576 protein glycosylation; • protein N-linked glycosylation; • oligosaccharide biosynthetic process;
	Izvori:Amigo / QuickGO
Ortolozi
	10317
	93961
	ENSG00000183778
	ENSMUSG00000074892
	Q9Y2C3
	Q9JI67
NM_033173; NM_001278650; NM_006057; NM_033170; NM_033171;
	NM_033172; NM_001356336; NM_001356338; NM_001356339
	NM_001122993; NM_033149; NM_001358389; NM_001358390
NP_001265579; NP_006048; NP_149360; NP_149361; NP_149362;
	NP_001343265; NP_001343267; NP_001343268
	NP_001116465; NP_149161; NP_001345318; NP_001345319
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Beta-1,3-galaktoziltransferaza 5 jest enzim koji je kod ljudi kodiran genom B3GALT5 sa hromosoma 21.^[5]^[6]

Aminokiselinska sekvenca uredi

Dužina polipeptidnog lanca je 310 aminokiselina, a molekulska težina 36.189 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MAFPKMRLMY	ICLLVLGALC	LYFSMYSLNP	FKEQSFVYKK	DGNFLKLPDT
DCRQTPPFLV	LLVTSSHKQL	AERMAIRQTW	GKERMVKGKQ	LKTFFLLGTT
SSAAETKEVD	QESQRHGDII	QKDFLDVYYN	LTLKTMMGIE	WVHRFCPQAA
FVMKTDSDMF	INVDYLTELL	LKKNRTTRFF	TGFLKLNEFP	IRQPFSKWFV
SKSEYPWDRY	PPFCSGTGYV	FSGDVASQVY	NVSKSVPYIK	LEDVFVGLCL
ERLNIRLEEL	HSQPTFFPGG	LRFSVCLFRR	IVACHFIKPR	TLLDYWQALE
NSRGEDCPPV

Funkcija uredi

Ovaj gen je član porodice gena beta-1,3-galaktoziltransferaza (beta3GalT). Porodica kodira tip II membranski vezanih glikoproteina sa različitim enzimskim funkcijama, koristeći različite donorske supstrate (UDP-galaktoza i UDP-N-acetilglukozamin) i različite akceptorske šećere (N-acetilglukozamin, galaktoza, N-acetilgalaktozamin). Beta3GalT geni su udaljeni srodnici gena Drosophila brainiak i imaju kodirajuću sekvencu proteina u jednom egzonu. Proteini beta3GalT također sadrže konzervirane sekvence koje se ne nalaze u beta4GalT ili alpha3GalT proteinima.

Ugljikohidratni lanci sintetizirani ovim enzimima označeni su kao tip 1, dok beta4GalT enzimi sintetiziraju lance ugljikohidrata tipa 2. Odnos lanaca tipa 1:tip 2 menja se tokom embriogeneze. Prema sličnosti sekvenci, geni beta3GalT spadaju u najmanje dvije grupe: beta3GalT4 i 4 druga beta3GalT gena (beta3GalT1-3, beta3GalT5). Ovaj gen kodira najvjerovatnijeg kandidata za sintezu tipa 1 Lewis antigena za koje se često nađe da su povišeni u gastrointestinalnom kanceru i raku gušterače. Kodirani protein je neaktivan sa N-vezanim glikoproteinima i funkcioniše u glikozilaciji mucin. Opisano je pet varijanti transkripta koje se razlikuju u 5' UTR. Sve varijante transkripta kodiraju identičan protein.^[6]

Reference uredi

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000183778 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000074892 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Isshiki S, Togayachi A, Kudo T, Nishihara S, Watanabe M, Kubota T, Kitajima M, Shiraishi N, Sasaki K, Andoh T, Narimatsu H (Jun 1999). "Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom". J Biol Chem. 274 (18): 12499–507. doi:10.1074/jbc.274.18.12499. PMID 10212226.
^ ^a ^b ^c "Entrez Gene: B3GALT5 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5".

Dopunska literatura uredi

Amado M, Almeida R, Schwientek T, Clausen H (2000). "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions". Biochim. Biophys. Acta. 1473 (1): 35–53. doi:10.1016/S0304-4165(99)00168-3. PMID 10580128.
Kalyanaraman VS, Rodriguez V, Veronese F, et al. (1990). "Characterization of the secreted, native gp120 and gp160 of the human immunodeficiency virus type 1". AIDS Res. Hum. Retroviruses. 6 (3): 371–80. doi:10.1089/aid.1990.6.371. PMID 2187500.
Pal R, Hoke GM, Sarngadharan MG (1989). "Role of oligosaccharides in the processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1". Proc. Natl. Acad. Sci. U.S.A. 86 (9): 3384–8. Bibcode:1989PNAS...86.3384P. doi:10.1073/pnas.86.9.3384. PMC 287137. PMID 2541446.
Dewar RL, Vasudevachari MB, Natarajan V, Salzman NP (1989). "Biosynthesis and processing of human immunodeficiency virus type 1 envelope glycoproteins: effects of monensin on glycosylation and transport". J. Virol. 63 (6): 2452–6. doi:10.1128/jvi.63.6.2452-2456.1989. PMC 250699. PMID 2542563.
Kozarsky K, Penman M, Basiripour L, et al. (1989). "Glycosylation and processing of the human immunodeficiency virus type 1 envelope protein". J. Acquir. Immune Defic. Syndr. 2 (2): 163–9. PMID 2649653.
Robinson WE, Montefiori DC, Mitchell WM (1988). "Evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (HIV-1) pathogenesis". AIDS Res. Hum. Retroviruses. 3 (3): 265–82. doi:10.1089/aid.1987.3.265. PMID 2829950.
Zhou D, Berger EG, Hennet T (1999). "Molecular cloning of a human UDP-galactose:GlcNAcbeta1,3GalNAc beta1, 3 galactosyltransferase gene encoding an O-linked core3-elongation enzyme". Eur. J. Biochem. 263 (2): 571–6. doi:10.1046/j.1432-1327.1999.00541.x. PMID 10406968.
Hattori M, Fujiyama A, Taylor TD, et al. (2000). "The DNA sequence of human chromosome 21". Nature. 405 (6784): 311–9. Bibcode:2000Natur.405..311H. doi:10.1038/35012518. PMID 10830953.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Dunn CA, Medstrand P, Mager DL (2004). "An endogenous retroviral long terminal repeat is the dominant promoter for human β1,3-galactosyltransferase 5 in the colon". Proc. Natl. Acad. Sci. U.S.A. 100 (22): 12841–6. doi:10.1073/pnas.2134464100. PMC 240706. PMID 14534330.
Sato T, Furukawa K (2004). "Transcriptional regulation of the human beta-1,4-galactosyltransferase V gene in cancer cells: essential role of transcription factor Sp1". J. Biol. Chem. 279 (38): 39574–83. doi:10.1074/jbc.M405805200. PMID 15263012.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Dunn CA, van de Lagemaat LN, Baillie GJ, Mager DL (2006). "Endogenous retrovirus long terminal repeats as ready-to-use mobile promoters: the case of primate beta3GAL-T5". Gene. 364: 2–12. doi:10.1016/j.gene.2005.05.045. PMID 16112824.
Hu YH, Warnatz HJ, Vanhecke D, et al. (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics. 7: 155. doi:10.1186/1471-2164-7-155. PMC 1526728. PMID 16780588.

Vanjski linkovi uredi

B3GALT5 lokacija ljudskog genoma UCSC Genome Browser.

B3GALT5 detalji ljudskog genoma u UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000183778 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000074892 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10212226-5] Isshiki S, Togayachi A, Kudo T, Nishihara S, Watanabe M, Kubota T, Kitajima M, Shiraishi N, Sasaki K, Andoh T, Narimatsu H (Jun 1999). "Cloning, expression, and characterization of a novel UDP-galactose:beta-N-acetylglucosamine beta1,3-galactosyltransferase (beta3Gal-T5) responsible for synthesis of type 1 chain in colorectal and pancreatic epithelia and tumor cells derived therefrom". J Biol Chem. 274 (18): 12499–507. doi:10.1074/jbc.274.18.12499. PMID 10212226.

[entrez-6] "Entrez Gene: B3GALT5 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5".

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