ARHGAP21

Rho GTPazni aktivirakići protein 21 jest protein koji je kod ljudi kodiran genom ARHGAP21.

ARHGAP21
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 2DHJ, 2J59, 2YUY
Identifikatori
Aliasi	ARHGAP21
Vanjski ID-jevi	OMIM: 609870 MGI: 1918685 HomoloGene: 10822 GeneCards: ARHGAP21
Lokacija gena (čovjek) Hrom. Hromosom 10 (čovjek) Bend 10p12.1|10p12.3 Početak 24,583,609 bp Kraj 24,723,668 bp
Lokacija gena (miš) Hrom. Hromosom 2 (miš) Bend 2|2 A3 Početak 20,852,730 bp Kraj 20,973,692 bp
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • GO:0005097, GO:0005099, GO:0005100 GTPase activator activity Ćelijska komponenta • Golđijev aparat • Golđijeva membrana • cytoplasmic vesicle membrane • membrana • GO:0016023 citoplazmatska vezikula • citoplazma • citoskelet • citosol • ćelijska membrana • actin cytoskeleton • međućelijske veze Biološki proces • establishment of Golgi localization • Golgi organization • organelle transport along microtubule • maintenance of Golgi location • GO:0072468 Transdukcija signala • GO:0032320, GO:0032321, GO:0032855, GO:0043089, GO:0032854 positive regulation of GTPase activity • regulation of small GTPase mediated signal transduction Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	57584	71435
Ensembl	ENSG00000107863	ENSMUSG00000036591
UniProt	Q5T5U3	Q6DFV3
RefSeq (mRNK)	NM_020824	NM_001081364 NM_001128084
RefSeq (bjelančevina)	NP_065875 NP_001354376 NP_001354377 NP_001354378 NP_001354379 NP_001354380 NP_001354381 NP_001354382 NP_001354383 NP_001354384	NP_001074833 NP_001121556 NP_001395248 NP_001395249 NP_001395250 NP_001395251 NP_001395252 NP_001395253 NP_001395254
Lokacija (UCSC)	Chr 10: 24.58 – 24.72 Mb	Chr 2: 20.85 – 20.97 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 1.957 aminokiselina, а molekulska težina 217.331 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MATRRTGLSE		GDGDKLKACE		VSKNKDGKEQ		SETVSLSEDE		TFSWPGPKTV
TLKRTSQGFG		FTLRHFIVYP		PESAIQFSYK		DEENGNRGGK		QRNRLEPMDT
IFVKQVKEGG		PAFEAGLCTG		DRIIKVNGES		VIGKTYSQVI		ALIQNSDTTL
ELSVMPKDED		ILQVLQFTKD		VTALAYSQDA		YLKGNEAYSG		NARNIPEPPP
ICYPWLPSAP		SAMAQPVEIS		PPDSSLSKQQ		TSTPVLTQPG		RAYRMEIQVP
PSPTDVAKSN		TAVCVCNESV		RTVIVPSEKV		VDLLSNRNNH		TGPSHRTEEV
RYGVSEQTSL		KTVSRTTSPP		LSIPTTHLIH		QPAGSRSLEP		SGILLKSGNY
SGHSDGISSS		RSQAVEAPSV		SVNHYSPNSH		QHIDWKNYKT		YKEYIDNRRL
HIGCRTIQER		LDSLRAASQS		TTDYNQVVPN		RTTLQGRRRS		TSHDRVPQSV
QIRQRSVSQE		RLEDSVLMKY		CPRSASQGAL		TSPSVSFSNH		RTRSWDYIEG
QDETLENVNS		GTPIPDSNGE		KKQTYKWSGF		TEQDDRRGIC		ERPRQQEIHK
SFRGSNFTVA		PSVVNSDNRR		MSGRGVGSVS		QFKKIPPDLK		TLQSNRNFQT
TCGMSLPRGI		SQDRSPLVKV		RSNSLKAPST		HVTKPSFSQK		SFVSIKDQRP
VNHLHQNSLL		NQQTWVRTDS		APDQQVETGK		SPSLSGASAK		PAPQSSENAG
TSDLELPVSQ		RNQDLSLQEA		ETEQSDTLDN		KEAVILREKP		PSGRQTPQPL
RHQSYILAVN		DQETGSDTTC		WLPNDARREV		HIKRMEERKA		SSTSPPGDSL
ASIPFIDEPT		SPSIDHDIAH		IPASAVISAS		TSQVPSIATV		PPCLTTSAPL
IRRQLSHDHE		SVGPPSLDAQ		PNSKTERSKS		YDEGLDDYRE		DAKLSFKHVS
SLKGIKIADS		QKSSEDSGSR		KDSSSEVFSD		AAKEGWLHFR		PLVTDKGKRV
GGSIRPWKQM		YVVLRGHSLY		LYKDKREQTT		PSEEEQPISV		NACLIDISYS
ETKRKNVFRL		TTSDCECLFQ		AEDRDDMLAW		IKTIQESSNL		NEEDTGVTNR
DLISRRIKEY		NNLMSKAEQL		PKTPRQSLSI		RQTLLGAKSE		PKTQSPHSPK
EESERKLLSK		DDTSPPKDKG		TWRKGIPSIM		RKTFEKKPTA		TGTFGVRLDD
CPPAHTNRYI		PLIVDICCKL		VEERGLEYTG		IYRVPGNNAA		ISSMQEELNK
GMADIDIQDD		KWRDLNVISS		LLKSFFRKLP		EPLFTNDKYA		DFIEANRKED
PLDRLKTLKR		LIHDLPEHHY		ETLKFLSAHL		KTVAENSEKN		KMEPRNLAIV
FGPTLVRTSE		DNMTHMVTHM		PDQYKIVETL		IQHHDWFFTE		EGAEEPLTTV
QEESTVDSQP		VPNIDHLLTN		IGRTGVSPGD		VSDSATSDST		KSKGSWGSGK
DQYSRELLVS		SIFAAASRKR		KKPKEKAQPS		SSEDELDNVF		FKKENVEQCH
NDTKEESKKE		SETLGRKQKI		IIAKENSTRK		DPSTTKDEKI		SLGKESTPSE
EPSPPHNSKH		NKSPTLSCRF		AILKESPRSL		LAQKSSHLEE		TGSDSGTLLS
TSSQASLARF		SMKKSTSPET		KHSEFLANVS		TITSDYSTTS		SATYLTSLDS
SRLSPEVQSV		AESKGDEADD		ERSELISEGR		PVETDSESEF		PVFPTALTSE
RLFRGKLQEV		TKSSRRNSEG		SELSCTEGSL		TSSLDSRRQL		FSSHKLIECD
TLSRKKSARF		KSDSGSLGDA		KNEKEAPSLT		KVFDVMKKGK		STGSLLTPTR
GESEKQEPTW		KTKIADRLKL		RPRAPADDMF		GVGNHKVNAE		TAKRKSIRRR
HTLGGHRDAT		EISVLNFWKV		HEQSGERESE		LSAVNRLKPK		CSAQDLSISD
WLARERLRTS		TSDLSRGEIG		DPQTENPSTR		EIATTDTPLS		LHCNTGSSSS
TLASTNRPLL		SIPPQSPDQI		NGESFQNVSK		NASSAANAQP		HKLSETPGSK
AEFHPCL

Funkcija

ARHGAP21 prvenstveno funkcionira kao protein koji aktivira GTPazu (GAP) za CDC42 (MIM 116952) i regulira kompleks Arp2/3 kompleks ARP2/3 (MIM 604221) i dinamiku F-aktina u Golgijevom aparatu, kontrolom aktivnosti CDC42 (Dubois et al., 2005 15793564). Postoji malo znanstvene literature o ARHGAP21, ali nedavni pregledi ističu da on ima važnu ulogu u citoskeletnim procesima kod raka, transportu tvari unutar ćelije i lučenju insulina

Dopunska literatura

• Bassères DS, Tizzei EV, Duarte AA, Costa FF, Saad ST (2002). "ARHGAP10, a novel human gene coding for a potentially cytoskeletal Rho-GTPase activating protein". Biochem. Biophys. Res. Commun. 294 (3): 579–85. doi:10.1016/S0006-291X(02)00514-4. PMID 12056806.
• Dubois T, Paléotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P (2005). "Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics". Nat. Cell Biol. 7 (4): 353–64. doi:10.1038/ncb1244. PMID 15793564. S2CID 37000096.
• Grupe A, Li Y, Rowland C, Nowotny P, Hinrichs AL, Smemo S, Kauwe JS, Maxwell TJ, Cherny S, Doil L, Tacey K, van Luchene R, Myers A, Wavrant-De Vrièze F, Kaleem M, Hollingworth P, Jehu L, Foy C, Archer N, Hamilton G, Holmans P, Morris CM, Catanese J, Sninsky J, White TJ, Powell J, Hardy J, O'Donovan M, Lovestone S, Jones L, Morris JC, Thal L, Owen M, Williams J, Goate A (2006). "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease". Am. J. Hum. Genet. 78 (1): 78–88. doi:10.1086/498851. PMC 1380225. PMID 16385451.
• Ménétrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A (2007). "Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes". EMBO J. 26 (7): 1953–62. doi:10.1038/sj.emboj.7601634. PMC 1847662. PMID 17347647.
• Bigarella CL, Borges L, Costa FF, Saad ST (2009). "ARHGAP21 modulates FAK activity and impairs glioblastoma cell migration". Biochim. Biophys. Acta. 1793 (5): 806–16. doi:10.1016/j.bbamcr.2009.02.010. PMID 19268501.
• Anthony DF, Sin YY, Vadrevu S, Advant N, Day JP, Byrne AM, Lynch MJ, Milligan G, Houslay MD, Baillie GS (2011). "β-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation". Mol. Cell. Biol. 31 (5): 1066–75. doi:10.1128/MCB.00883-10. PMC 3067824. PMID 21173159.
• Wang S, Li H, Chen Y, Wei H, Gao GF, Liu H, Huang S, Chen JL (2012). "Transport of influenza virus neuraminidase (NA) to host cell surface is regulated by ARHGAP21 and Cdc42 proteins". J. Biol. Chem. 287 (13): 9804–16. doi:10.1074/jbc.M111.312959. PMC 3323004. PMID 22318733.
• Bigarella CL, Ferro KP, Barcellos KS, Martins-de-Souza D, Traina F, Novello JC, Saad ST, Archangelo LF (2012). "Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3". FEBS Lett. 586 (19): 3522–8. doi:10.1016/j.febslet.2012.08.012. PMID 22922005. S2CID 35663132.
• Barcellos KS, Bigarella CL, Wagner MV, Vieira KP, Lazarini M, Langford PR, Machado-Neto JA, Call SG, Staley DM, Chung JY, Hansen MD, Saad ST (2013). "ARHGAP21 protein, a new partner of α-tubulin involved in cell-cell adhesion formation and essential for epithelial-mesenchymal transition". J. Biol. Chem. 288 (4): 2179–89. doi:10.1074/jbc.M112.432716. PMC 3554890. PMID 23235160.