UBR2

E3 ubikvitin-protein ligaza UBR2 jest enzim koji je kod ljudi kodiran genom UBR2.

UBR2
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 3NY2, 3NY3
Identifikatori
Aliasi	UBR2
Vanjski ID-jevi	OMIM: 609134 MGI: 1861099 HomoloGene: 26151 GeneCards: UBR2
Lokacija gena (čovjek) Hrom. Hromosom 6 (čovjek) Bend 6p21.1 Početak 42,564,029 bp Kraj 42,693,505 bp
Lokacija gena (miš) Hrom. Hromosom 17 (miš) Bend 17|17 C Početak 47,239,221 bp Kraj 47,321,482 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • vezivanje iona cinka • leucine binding • GO:1904264, GO:1904822, GO:0090622, GO:0090302 ubiquitin protein ligase activity • vezivanje iona metala • GO:0050372 aktivnost sa transferazom ubikvitina • aktivnost sa transferazom Ćelijska komponenta • Hromatin • jedro • kompleks ubikvitin-ligaze • citoplazma • citosol Biološki proces • cellular response to leucine • GO:0044257 protein catabolic process • male meiosis I • ubiquitin-dependent protein catabolic process • protein ubiquitination • ubiquitin-dependent protein catabolic process via the N-end rule pathway • negative regulation of TOR signaling • histone H2A ubiquitination • protein polyubiquitination • reciprocal meiotic recombination • male meiotic nuclear division • Spermatogeneza • negative regulation of transposition Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	23304	224826
Ensembl	ENSG00000024048	ENSMUSG00000023977
UniProt	Q8IWV8	Q6WKZ8
RefSeq (mRNK)	NM_001184801 NM_015255 NM_001363705	NM_001177374 NM_146078
RefSeq (bjelančevina)	NP_001171730 NP_056070 NP_001350634	NP_001170845 NP_666190
Lokacija (UCSC)	Chr 6: 42.56 – 42.69 Mb	Chr 17: 47.24 – 47.32 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 1.755 aminokiselina, а molekulska težina 200.538 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MASELEPEVQ		AIDRSLLECS		AEEIAGKWLQ		ATDLTREVYQ		HLAHYVPKIY
CRGPNPFPQK		EDMLAQHVLL		GPMEWYLCGE		DPAFGFPKLE		QANKPSHLCG
RVFKVGEPTY		SCRDCAVDPT		CVLCMECFLG		SIHRDHRYRM		TTSGGGGFCD
CGDTEAWKEG		PYCQKHELNT		SEIEEEEDPL		VHLSEDVIAR		TYNIFAITFR
YAVEILTWEK		ESELPADLEM		VEKSDTYYCM		LFNDEVHTYE		QVIYTLQKAV
NCTQKEAIGF		ATTVDRDGRR		SVRYGDFQYC		EQAKSVIVRN		TSRQTKPLKV
QVMHSSIVAH		QNFGLKLLSW		LGSIIGYSDG		LRRILCQVGL		QEGPDGENSS
LVDRLMLSDS		KLWKGARSVY		HQLFMSSLLM		DLKYKKLFAV		RFAKNYQQLQ
RDFMEDDHER		AVSVTALSVQ		FFTAPTLARM		LITEENLMSI		IIKTFMDHLR
HRDAQGRFQF		ERYTALQAFK		FRRVQSLILD		LKYVLISKPT		EWSDELRQKF
LEGFDAFLEL		LKCMQGMDPI		TRQVGQHIEM		EPEWEAAFTL		QMKLTHVISM
MQDWCASDEK		VLIEAYKKCL		AVLMQCHGGY		TDGEQPITLS		ICGHSVETIR
YCVSQEKVSI		HLPVSRLLAG		LHVLLSKSEV		AYKFPELLPL		SELSPPMLIE
HPLRCLVLCA		QVHAGMWRRN		GFSLVNQIYY		YHNVKCRREM		FDKDVVMLQT
GVSMMDPNHF		LMIMLSRFEL		YQIFSTPDYG		KRFSSEITHK		DVVQQNNTLI
EEMLYLIIML		VGERFSPGVG		QVNATDEIKR		EIIHQLSIKP		MAHSELVKSL
PEDENKETGM		ESVIEAVAHF		KKPGLTGRGM		YELKPECAKE		FNLYFYHFSR
AEQSKAEEAQ		RKLKRQNRED		TALPPPVLPP		FCPLFASLVN		ILQSDVMLCI
MGTILQWAVE		HNGYAWSESM		LQRVLHLIGM		ALQEEKQHLE		NVTEEHVVTF
TFTQKISKPG		EAPKNSPSIL		AMLETLQNAP		YLEVHKDMIR		WILKTFNAVK
KMRESSPTSP		VAETEGTIME		ESSRDKDKAE		RKRKAEIARL		RREKIMAQMS
EMQRHFIDEN		KELFQQTLEL		DASTSAVLDH		SPVASDMTLT		ALGPAQTQVP
EQRQFVTCIL		CQEEQEVKVE		SRAMVLAAFV		QRSTVLSKNR		SKFIQDPEKY
DPLFMHPDLS		CGTHTSSCGH		IMHAHCWQRY		FDSVQAKEQR		RQQRLRLHTS
YDVENGEFLC		PLCECLSNTV		IPLLLPPRNI		FNNRLNFSDQ		PNLTQWIRTI
SQQIKALQFL		RKEESTPNNA		STKNSENVDE		LQLPEGFRPD		FRPKIPYSES
IKEMLTTFGT		ATYKVGLKVH		PNEEDPRVPI		MCWGSCAYTI		QSIERILSDE
DKPLFGPLPC		RLDDCLRSLT		RFAAAHWTVA		SVSVVQGHFC		KLFASLVPND
SHEELPCILD		IDMFHLLVGL		VLAFPALQCQ		DFSGISLGTG		DLHIFHLVTM
AHIIQILLTS		CTEENGMDQE		NPPCEEESAV		LALYKTLHQY		TGSALKEIPS
GWHLWRSVRA		GIMPFLKCSA		LFFHYLNGVP		SPPDIQVPGT		SHFEHLCSYL
SLPNNLICLF		QENSEIMNSL		IESWCRNSEV		KRYLEGERDA		IRYPRESNKL
INLPEDYSSL		INQASNFSCP		KSGGDKSRAP		TLCLVCGSLL		CSQSYCCQTE
LEGEDVGACT		AHTYSCGSGV		GIFLRVRECQ		VLFLAGKTKG		CFYSPPYLDD
YGETDQGLRR		GNPLHLCKER		FKKIQKLWHQ		HSVTEEIGHA		QEANQTLVGI
DWQHL

Funkcija

Proteoliza pomoću ubikvitin-proteasomskog sistema kontrolira koncentraciju mnogih regulatornih proteina. Selektivnost ubikvitilacija određena je ubikvitinskim E3-ligazama, koje prepoznaju signal destabilizacije supstrata, ili degron. E3-ligaza UBR2 učestvuje na putu N-kraja, koji cilja na proteine koji nose N-terminalni degron ili N-degron (Kwon et al., 2003; prema OMIM-u.)

Dopunska literatura

• Nagase T, Ishikawa K, Nakajima D, et al. (1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
• Kwon YT, Xia Z, An JY, et al. (2003). "Female Lethality and Apoptosis of Spermatocytes in Mice Lacking the UBR2 Ubiquitin Ligase of the N-End Rule Pathway". Mol. Cell. Biol. 23 (22): 8255–71. doi:10.1128/MCB.23.22.8255-8271.2003. PMC 262401. PMID 14585983.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Yin J, Kwon YT, Varshavsky A, Wang W (2005). "RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway". Hum. Mol. Genet. 13 (20): 2421–30. doi:10.1093/hmg/ddh269. PMID 15317757.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kwak KS, Zhou X, Solomon V, et al. (2005). "Regulation of protein catabolism by muscle-specific and cytokine-inducible ubiquitin ligase E3alpha-II during cancer cachexia". Cancer Res. 64 (22): 8193–8. doi:10.1158/0008-5472.CAN-04-2102. PMID 15548684.
• Zenker M, Mayerle J, Lerch MM, et al. (2006). "Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome)" (PDF). Nat. Genet. 37 (12): 1345–50. doi:10.1038/ng1681. PMID 16311597. S2CID 23050042. Arhivirano s originala (PDF), 17. 10. 2022. Pristupljeno 5. 10. 2021.
• Matta-Camacho E, Kozlov G, Li F, Gehring K, et al. (2010). "Structural basis of substrate recognition and specificity in the N-end rule pathway". Nat Struct Mol Biol. 17 (10): 1182–7. doi:10.1038/nsmb.1894. PMID 20835242. S2CID 20961853.

Vanjski linkovi

• Q8IWV8