RNF34

E3 ubikvitin-proteinska ligaza RNF34 je enzim koji je kod ljudi kodiran genom RNF34.

RNF34
Identifikatori
Aliasi	RNF34
Vanjski ID-jevi	OMIM: 608299 MGI: 2153340 HomoloGene: 11839 GeneCards: RNF34
Lokacija gena (čovjek) Hrom. Hromosom 12 (čovjek) Bend 12q24.31 Početak 121,400,083 bp Kraj 121,430,623 bp
Lokacija gena (miš) Hrom. Hromosom 5 (miš) Bend 5|5 F Početak 122,988,111 bp Kraj 123,009,354 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • vezivanje iona metala • phosphatidylinositol phosphate binding • p53 binding • ubiquitin protein ligase binding • GO:0001948, GO:0016582 vezivanje za proteine • GO:1904264, GO:1904822, GO:0090622, GO:0090302 ubiquitin protein ligase activity • GO:0050372 aktivnost sa transferazom ubikvitina • aktivnost sa transferazom Ćelijska komponenta • citoplazma • Endomembranski sistem • jedro • membrana • ćelijska membrana • nuclear speck • nukleoplazma • citosol • nuclear body Biološki proces • negative regulation of signal transduction by p53 class mediator • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway • protein K48-linked ubiquitination • negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis • GO:0097285 apoptoza • regulation of oxygen metabolic process • cellular response to cold • protein ubiquitination • proteasome-mediated ubiquitin-dependent protein catabolic process • ubiquitin-dependent protein catabolic process Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	80196	80751
Ensembl	ENSG00000170633	ENSMUSG00000029474
UniProt	Q969K3	Q99KR6
RefSeq (mRNK)	NM_194271 NM_001256858 NM_025126 NM_001394208	NM_030564
RefSeq (bjelančevina)	NP_001243787 NP_079402 NP_919247	NP_085041
Lokacija (UCSC)	Chr 12: 121.4 – 121.43 Mb	Chr 5: 122.99 – 123.01 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Protein kodiran ovim genom sadrži RINF prst, motiv za koji se zna da je uključen u interakcije protein-protein i protein-DNK. Ovaj protein stupa u interakciju s DNAJA3/hTid-1, koji je protein DnaJ, za koji se izvještava da funkcionira kao modulator apoptoze. Pokazalo se da pretjerana ekspresija ovog gena u HeLa ćelijama daje otpornost na apoptozu induciranu TNF-alfa, što ukazuje na anti-apoptotičku funkciju ovog proteina. Ovaj protein se može cijepati kaspazom-3 tokom indukcije apoptoze. Prijavljene su i alternativno prerađene varijante transkripta koje kodiraju različite izoforme.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 372 aminokiseline, a molekulska težina 41.641 Da.

10		20		30		40		50
MKAGATSMWA		SCCGLLNEVM		GTGAVRGQQS		AFAGATGPFR		FTPNPEFSTY
PPAATEGPNI		VCKACGLSFS		VFRKKHVCCD		CKKDFCSVCS		VLQENLRRCS
TCHLLQETAF		QRPQLMRLKV		KDLRQYLILR		NIPIDTCREK		EDLVDLVLCH
HGLGSEDDMD		TSSLNSSRSQ		TSSFFTRSFF		SNYTAPSATM		SSFQGELMDG
DQTSRSGVPA		QVQSEITSAN		TEDDDDDDDE		DDDDEEENAE		DRNPGLSKER
VRASLSDLSS		LDDVEGMSVR		QLKEILARNF		VNYSGCCEKW		ELVEKVNRLY
KENEENQKSY		GERLQLQDEE		DDSLCRICMD		AVIDCVLLEC		GHMVTCTKCG
KRMSECPICR		QYVVRAVHVF		KS

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Dopunska literatura

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• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
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• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Sasaki S, Watanabe T, Konishi T, et al. (2005). "Effects of expression of hRFI on adenoma formation and tumor progression in colorectal adenoma-carcinoma sequence". J. Exp. Clin. Cancer Res. 23 (3): 507–12. PMID 15595643.
• Konishi T, Sasaki S, Watanabe T, et al. (2005). "Overexpression of hRFI (human ring finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells". Mol. Cancer Ther. 4 (5): 743–50. doi:10.1158/1535-7163.MCT-05-0020. PMID 15897238.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.