PDXK

PDXK
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2AJP, 2F7K, 2YXT, 2YXU, 3FHX, 3FHY, 3KEU, 4EN4, 4EOH
Identifikatori
Aliasi	PDXK
Vanjski ID-jevi	OMIM: 179020 MGI: 1351869 HomoloGene: 2731 GeneCards: PDXK
Lokacija gena (čovjek)
Hrom.	Hromosom 21 (čovjek)
Kraj	43,762,307 bp
Lokacija gena (miš)
Hrom.	Hromosom 10 (miš)
Kraj	78,300,809 bp
Obrazac RNK ekspresije
	; ; ; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• aktivnost sa transferazom; • nucleotide binding; • protein homodimerization activity; • pyridoxal kinase activity; • vezivanje iona cinka; • potassium ion binding; • sodium ion binding; • vezivanje iona metala; • kinase activity; • lithium ion binding; • pyridoxal phosphate binding; • ATP binding; • magnesium ion binding;
Ćelijska komponenta	• citosol; • Egzosom; • jedro; • extracellular region; • citoplazma; • secretory granule lumen; • specific granule lumen; • nukleoplazma;
Biološki proces	• pyridoxal phosphate biosynthetic process; • Fosforilacija; • pyridoxal 5'-phosphate salvage; • Ćelijska proliferacija; • neutrophil degranulation; • vitamin B6 metabolic process;
	Izvori:Amigo / QuickGO
Ortolozi
	8566
	216134
	ENSG00000160209
	ENSMUSG00000032788
	O00764
	Q8K183
	NM_003681; NM_021941; NM_001331030
	NM_172134
	NP_001317959; NP_003672
	NP_742146
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Piridoksal-kinaza jest enzim koji je kod ljudi kodiran genom PDXK sa hromosoma 21.^[5]^[6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 312 aminokiselina, a molekulska težina 35.102 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MEEECRVLSI	QSHVIRGYVG	NRAATFPLQV	LGFEIDAVNS	VQFSNHTGYA
HWKGQVLNSD	ELQELYEGLR	LNNMNKYDYV	LTGYTRDKSF	LAMVVDIVQE
LKQQNPRLVY	VCDPVLGDKW	DGEGSMYVPE	DLLPVYKEKV	VPLADIITPN
QFEAELLSGR	KIHSQEEALR	VMDMLHSMGP	DTVVITSSDL	PSPQGSNYLI
VLGSQRRRNP	AGSVVMERIR	MDIRKVDAVF	VGTGDLFAAM	LLAWTHKHPN
NLKVACEKTV	STLHHVLQRT	IQCAKAQAGE	GVRPSPMQLE	LRMVQSKRDI
EDPEIVVQAT	VL

Funkcija

Protein kodiran ovim genom fosforilira vitamin B₆, korak potreban za konverziju vitamina B₆ u piridoksal-5-fosfat, važan kofaktor u posrednom metabolizmu. Kodirani protein je citoplazmatski] i vjerovatno djeluje kao homodimer. Opisane su alternativno prerađene varijante transkripta, ali njihova biološka valjanost nije utvrđena.^[6]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000160209 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032788 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Hanna MC, Turner AJ, Kirkness EF (May 1997). "Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor". J Biol Chem. 272 (16): 10756–60. doi:10.1074/jbc.272.16.10756. PMID 9099727.
^ ^a ^b ^c "Entrez Gene: PDXK pyridoxal (pyridoxine, vitamin B₆) kinase".

Dopunska literatura

Chern CJ, Beutler E (1976). "Biochemical and electrophoretic studies of erythrocyte pyridoxine kinase in white and black Americans". Am. J. Hum. Genet. 28 (1): 9–17. PMC 1684914. PMID 2009.
Merrill AH, Henderson JM, Wang E, et al. (1984). "Metabolism of vitamin B-6 by human liver". J. Nutr. 114 (9): 1664–74. doi:10.1093/jn/114.9.1664. PMID 6088736.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Zhang Z, Smith E, Surowiec SM, et al. (1993). "Synthesis of N-(4'-pyridoxyl)sphingosine and its uptake and metabolism by isolated cells". Membrane Biochemistry. 10 (1): 53–9. doi:10.3109/09687689309150252. PMID 8510562.
Lainé-Cessac P, Cailleux A, Allain P (1997). "Mechanisms of the inhibition of human erythrocyte pyridoxal kinase by drugs". Biochem. Pharmacol. 54 (8): 863–70. doi:10.1016/S0006-2952(97)00252-9. PMID 9354586.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Hattori M, Fujiyama A, Taylor TD, et al. (2000). "The DNA sequence of human chromosome 21". Nature. 405 (6784): 311–9. Bibcode:2000Natur.405..311H. doi:10.1038/35012518. PMID 10830953.
Lee HS, Moon BJ, Choi SY, Kwon OS (2001). "Human pyridoxal kinase: overexpression and properties of the recombinant enzyme". Mol. Cells. 10 (4): 452–9. PMID 10987144.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Shin JH, Weitzdoerfer R, Fountoulakis M, Lubec G (2004). "Expression of cystathionine beta-synthase, pyridoxal kinase, and ES1 protein homolog (mitochondrial precursor) in fetal Down syndrome brain". Neurochem. Int. 45 (1): 73–9. doi:10.1016/j.neuint.2003.12.004. PMID 15082224. S2CID 20620865.
Fang X, Zhou ZM, Lu L, et al. (2004). "Expression of a novel pyridoxal kinase mRNA splice variant, PKH-T, in human testis". Asian J. Androl. 6 (2): 83–91. PMID 15154080.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wang L, Zhu YF, Guo XJ, et al. (2006). "A two-dimensional electrophoresis reference map of human ovary". J. Mol. Med. 83 (10): 812–21. doi:10.1007/s00109-005-0676-y. PMID 16021519. S2CID 6644433.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
Flanagan JM, Beutler E (2006). "The genetic basis of human erythrocyte pyridoxal kinase activity variation". Haematologica. 91 (6): 801–4. PMID 16704963.
Hu YH, Warnatz HJ, Vanhecke D, et al. (2006). "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins". BMC Genomics. 7: 155. doi:10.1186/1471-2164-7-155. PMC 1526728. PMID 16780588.
Musayev FN, di Salvo ML, Ko TP, et al. (2007). "Crystal Structure of human pyridoxal kinase: Structural basis of M+ and M2+ activation". Protein Sci. 16 (10): 2184–94. doi:10.1110/ps.073022107. PMC 2204131. PMID 17766369.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000160209 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000032788 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9099727-5] Hanna MC, Turner AJ, Kirkness EF (May 1997). "Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor". J Biol Chem. 272 (16): 10756–60. doi:10.1074/jbc.272.16.10756. PMID 9099727.

[entrez-6] "Entrez Gene: PDXK pyridoxal (pyridoxine, vitamin B₆) kinase".

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