Aminokiselinska sekvenca
uredi
Dužina polipeptidnog lanca je 1.146 aminokiselina , а molekulska težina 127.593 Da .[ 8]
10 20 30 40 50 MGPAPAGEQL RGATGEPEVM EPALEGTGKE GKKASSRKRT LAEPPAKGLL QPVKLSRAEL YKEPTNEELN RLRETEILFH SSLLRLQVEE LLKEVRLSEK KKDRIDAFLR EVNQRVVRVP SVPETELTDQ AWLPAGVRVP LHQVPYAVKG CFRFLPPAQV TVVGSYLLGT CIRPDINVDV ALTMPREILQ DKDGLNQRYF RKRALYLAHL AHHLAQDPLF GSVCFSYTNG CHLKPSLLLR PRGKDERLVT VRLHPCPPPD FFRPCRLLPT KNNVRSAWYR GQSPAGDGSP EPPTPRYNTW VLQDTVLESH LQLLSTILSS AQGLKDGVAL LKVWLRQREL DKGQGGFTGF LVSMLVVFLV STRKIHTTMS GYQVLRSVLQ FLATTDLTVN GISLCLSSDP SLPALADFHQ AFSVVFLDSS GHLNLCADVT ASTYHQVQHE ARLSMMLLDS RADDGFHLLL MTPKPMIRAF DHVLHLRPLS RLQAACHRLK LWPELQDNGG DYVSAALGPL TTLLEQGLGA RLNLLAHSRP PVPEWDISQD PPKHKDSGTL TLGLLLRPEG LTSVLELGPE ADQPEAAKFR QFWGSRSELR RFQDGAIREA VVWEAASMSQ KRLIPHQVVT HLLALHADIP ETCVHYVGGP LDALIQGLKE TSSTGEEALV AAVRCYDDLS RLLWGLEGLP LTVSAVQGAH PVLRYTEVFP PTPVRPAFSF YETLRERSSL LPRLDKPCPA YVEPMTVVCH LEGSGQWPQD AEAVQRVRAA FQLRLAELLT QQHGLQCRAT ATHTDVLKDG FVFRIRVAYQ REPQILKEVQ SPEGMISLRD TAASLRLERD TRQLPLLTSA LHGLQQQHPA FSGVARLAKR WVRAQLLGEG FADESLDLVA AALFLHPEPF TPPSSPQVGF LRFLFLVSTF DWKNNPLFVN LNNELTVEEQ VEIRSGFLAA RAQLPVMVIV TPQDRKNSVW TQDGPSAQIL QQLVVLAAEA LPMLEKQLMD PRGPGDIRTV FRPPLDIYDV LIRLSPRHIP RHRQAVDSPA ASFCRGLLSQ PGPSSLMPVL GYDPPQLYLT QLREAFGDLA LFFYDQHGGE VIGVLWKPTS FQPQPFKASS TKGRMVMSRG GELVMVPNVE AILEDFAVLG EGLVQTVEAR SERWTV
Jedarce je gusta subjedarna membranska organela koja se okuplja oko klastera gena rRNK i funkcionira u biogenezi ribosoma . Ovaj gen kodira jedarčani protein povezan s RNK koji je visoko konzerviran među vrstama . Tretman permeabiliziranih ćrlija Rnazama pokazuje da je nukleolusna lokalizacija ovisna o RNK. Daljnje studije ukazuju da je protein povezan s biogenezom ribosoma kroz interakciju s primarnim transkriptima pre-rRNK. Na ovom mjestu postoji alternativna prerada i identificirane su dvije prerađene varijante koje kodiraju različite izoforme .[ 7]
^ a b c GRCh38: Ensembl release 89: ENSG00000165271 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028430 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Utama B, Kennedy D, Ru K, Mattick JS (Mar 2002). "Isolation and characterization of a new nucleolar protein, Nrap, that is conserved from yeast to humans" . Genes Cells . 7 (2): 115–32. doi :10.1046/j.1356-9597.2001.00507.x PMID 11895476 . S2CID 21385000 . ^ Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ (Dec 2004). "The small-subunit processome is a ribosome assembly intermediate" . Eukaryot Cell . 3 (6): 1619–26. doi :10.1128/EC.3.6.1619-1626.2004 . PMC 539036 PMID 15590835 . ^ a b "Entrez Gene: NOL6 nucleolar protein family 6 (RNA-associated)" .^ "UniProt, Q9H6R4" . Pristupljeno 10. 9. 2021 .
Dopunska literatura
uredi
Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus" . Curr. Biol . 12 (1): 1–11. doi :10.1016/S0960-9822(01)00650-9 PMID 11790298 . S2CID 14132033 . Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus" . Mol. Biol. Cell . 13 (11): 4100–9. doi :10.1091/mbc.E02-05-0271 . PMC 133617 PMID 12429849 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 . PMC 139241 PMID 12477932 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9" . Nature . 429 (6990): 369–74. Bibcode :2004Natur.429..369H . doi :10.1038/nature02465 . PMC 2734081 PMID 15164053 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature . 433 (7021): 77–83. Bibcode :2005Natur.433...77A . doi :10.1038/nature03207 . PMID 15635413 . S2CID 4344740 . Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle" . Proc. Natl. Acad. Sci. U.S.A . 103 (14): 5391–6. Bibcode :2006PNAS..103.5391N . doi :10.1073/pnas.0507066103 . PMC 1459365 PMID 16565220 . 
