GTPBP4
Jedarcetov GTP-vezujući protein 1 je protein koji je kod ljudi kodiran genom GTPBP4.[5][6]
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 634 aminokiseline, а molekulska težina 73.964 Da.[7]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAHYNFKKIT | VVPSAKDFID | LTLSKTQRKT | PTVIHKHYQI | HRIRHFYMRK | ||||
| VKFTQQNYHD | RLSQILTDFP | KLDDIHPFYA | DLMNILYDKD | HYKLALGQIN | ||||
| IAKNLVDNVA | KDYVRLMKYG | DSLYRCKQLK | RAALGRMCTV | IKRQKQSLEY | ||||
| LEQVRQHLSR | LPTIDPNTRT | LLLCGYPNVG | KSSFINKVTR | ADVDVQPYAF | ||||
| TTKSLFVGHM | DYKYLRWQVV | DTPGILDHPL | EDRNTIEMQA | ITALAHLRAA | ||||
| VLYVMDLSEQ | CGHGLREQLE | LFQNIRPLFI | NKPLIVVANK | CDVKRIAELS | ||||
| EDDQKIFTDL | QSEGFPVIET | STLTEEGVIK | VKTEACDRLL | AHRVETKMKG | ||||
| NKVNEVLNRL | HLAIPTRRDD | KERPPFIPEG | VVARRKRMET | EESRKKRERD | ||||
| LELEMGDDYI | LDLQKYWDLM | NLSEKHDKIP | EIWEGHNIAD | YIDPAIMKKL | ||||
| EELEKEEELR | TAAGEYDSVS | ESEDEEMLEI | RQLAKQIREK | KKLKILESKE | ||||
| KNTQGPRMPR | TAKKVQRTVL | EKEMRSLGVD | MDDKDDAHYA | VQARRSRSIT | ||||
| RKRKREDSAP | PSSVARSGSC | SRTPRDVSGL | RDVKMVKKAK | TMMKNAQKKM | ||||
| NRLGKKGEAD | RHVFDMKPKH | LLSGKRKAGK | KDRR |
Funkcija uredi
GTPaze funkcioniraju kao molekulni prekidači koji se mogu prebacivati između dva stanja: aktivno, kada je GTP vezan, i neaktivno, kada je vezan BDP. 'Aktivno' obično znači da molekula djeluje kao signal za pokretanje drugih događaja u ćeliji. Kad se vanćelijski ligand veže za G-protein spregnuti receptor, receptor mijenja svoju konformaciju i uključuje trimerne G-proteine, koji se povezuju s njim, izazivajući ih da izbace svoj BDP i zamijene ga GTP-om. Prekidač se isključuje kada G-protein hidrolizira svoj vezani GTP, pretvarajući ga nazad u BDP. No, prije nego što se to dogodi, aktivni protein ima priliku difundirati dalje od receptora i prenositi svoju poruku na duži period do svog nizvodnog cilja.[6]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000107937 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000021149 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Laping NJ, Olson BA, Zhu Y (Apr 2001). "Identification of a novel nuclear guanosine triphosphate-binding protein differentially expressed in renal disease". J Am Soc Nephrol. 12 (5): 883–90. doi:10.1681/ASN.V125883. PMID 11316846.
- ^ a b "Entrez Gene: GTPBP4 GTP binding protein 4".
- ^ "UniProt, Q9BZE4". Pristupljeno 3. 9. 2021.
Dopunska literatura uredi
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
- Lee H, Kim D, Dan HC, et al. (2007). "Identification and characterization of putative tumor suppressor NGB, a GTP-binding protein that interacts with the neurofibromatosis 2 protein". Mol. Cell. Biol. 27 (6): 2103–19. doi:10.1128/MCB.00572-06. PMC 1820506. PMID 17210637.
- Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.