ELL3

ELL3
Identifikatori
Aliasi	ELL3
Vanjski ID-jevi	OMIM: 609885 MGI: 2673679 HomoloGene: 11859 GeneCards: ELL3
Lokacija gena (čovjek)
Hrom.	Hromosom 15 (čovjek)
Kraj	43,777,315 bp
Lokacija gena (miš)
Hrom.	Hromosom 2 (miš)
Kraj	121,274,759 bp
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• Jedarce; • transcription elongation factor complex; • jedro; • nukleoplazma;
Biološki proces	• positive regulation of DNA-templated transcription, elongation; • positive regulation of neurogenesis; • positive regulation of neural precursor cell proliferation; • transcription elongation from RNA polymerase II promoter; • regulation of epithelial to mesenchymal transition; • DNA-templated transcription, elongation; • GO:0009373 regulation of transcription, DNA-templated; • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; • transcription by RNA polymerase II; • Spermatogeneza; • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II; • stem cell differentiation; • transcription, DNA-templated; • negative regulation of signal transduction by p53 class mediator; • snRNA transcription by RNA polymerase II;
	Izvori:Amigo / QuickGO
Ortolozi
	80237
	269344
	ENSG00000128886
	ENSMUSG00000027246
	Q9HB65
	Q80VR2
	NM_025165
	NM_145973
	NP_079441
	NP_666085
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Elongacijski faktor 3 sličan RNK-polimerazi II je protein koji je kod ljudi kodiran genom ELL3.^[5]

Međunarodni konzorcij za radijacijsko mapiranje locirao je gen ELL3 na hromosomu 15 (RH44309).

Aminokiselinska sekvenca uredi

Dužina polipeptidnog lanca je 397 aminokiselina, a molekulska težina 45.361 Da.^[6].

10	20	30	40	50
MEELQEPLRG	QLRLCFTQAA	RTSLLLLRLN	DAALRALQEC	QRQQVRPVIA
FQGHRGYLRL	PGPGWSCLFS	FIVSQCCQEG	AGGSLDLVCQ	RFLRSGPNSL
HCLGSLRERL	IIWAAMDSIP	APSSVQGHNL	TEDARHPESW	QNTGGYSEGD
AVSQPQMALE	EVSVSDPLAS	NQGQSLPGSS	REHMAQWEVR	SQTHVPNREP
VQALPSSASR	KRLDKKRSVP	VATVELEEKR	FRTLPLVPSP	LQGLTNQDLQ
EGEDWEQEDE	DMDPRLEHSS	SVQEDSESPS	PEDIPDYLLQ	YRAIHSAEQQ
HAYEQDFETD	YAEYRILHAR	VGTASQRFIE	LGAEIKRVRR	GTPEYKVLED
KIIQEYKKFR	KQYPSYREEK	RRCEYLHQKL	SHIKGLILEF	EEKNRGS

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija uredi

Pretragom baze podataka EST, Miller et al. (2000) identificirali su sekvence koje pokazuju visoku homologiju sa C-terminalnim sekvencama ELL i ELL2 . Koristeći strategiju zasnovanu na PCR-u, klonirali su ljudsku cDNA pune dužine, koju su označili kao ELL3. Izvedeni približno 400-aminokiselinski protein dijeli oko 50% identiteta sekvence s ELL i ELL2. Northern blot analizom otkriven je transkript od 2 kb eksprimiran isključivo u sjemenicima. Studije imunofluorescencije lokalizirale su ELL3 u jedru.

Funkcija gena uredi

Miller et al. (2000) utvrdili su da ELL3, poput ELL i ELL2, može povećati katalitsku brzinu izduživanja transkripcije RNK-polimerazom II.^[7]

Reference uredi

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000128886 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000027246 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: Elongation factor RNA polymerase II-like 3".
^ "UniProt, Q9HB65". Pristupljeno 13. 7. 2021.
^ Miller, T., Williams, K., Johnstone, R. W., Shilatifard, A. Identification, cloning, expression, and biochemical characterization of the testis-specific RNA polymerase II elongation factor ELL3. J. Biol. Chem. 275: 32052-32056, 2000. PubMed: 10882741

Dopunska literatura uredi

Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW (juli 2011). "Human mediator subunit MED26 functions as a docking site for transcription elongation factors". Cell. 146 (1): 92–104. doi:10.1016/j.cell.2011.06.005. PMC 3145325. PMID 21729782.
Park G, Gong Z, Chen J, Kim JE (april 2010). "Characterization of the DOT1L network: implications of diverse roles for DOT1L". The Protein Journal. 29 (3): 213–23. doi:10.1007/s10930-010-9242-8. PMID 20431927.
Miller T, Williams K, Johnstone RW, Shilatifard A (oktobar 2000). "Identification, cloning, expression, and biochemical characterization of the testis-specific RNA polymerase II elongation factor ELL3". The Journal of Biological Chemistry. 275 (41): 32052–6. doi:10.1074/jbc.M005175200. PMID 10882741.
Law IK, Liu L, Xu A, Lam KS, Vanhoutte PM, Che CM, Leung PT, Wang Y (maj 2009). "Identification and characterization of proteins interacting with SIRT1 and SIRT3: implications in the anti-aging and metabolic effects of sirtuins". Proteomics. 9 (9): 2444–56. doi:10.1002/pmic.200800738. PMID 19343720.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000128886 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000027246 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: Elongation factor RNA polymerase II-like 3".

[UniProt-6] "UniProt, Q9HB65". Pristupljeno 13. 7. 2021.

[7] Miller, T., Williams, K., Johnstone, R. W., Shilatifard, A. Identification, cloning, expression, and biochemical characterization of the testis-specific RNA polymerase II elongation factor ELL3. J. Biol. Chem. 275: 32052-32056, 2000. PubMed: 10882741

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