ELL3

Elongacijski faktor 3 sličan RNK-polimerazi II je protein koji je kod ljudi kodiran genom ELL3.

ELL3
Identifikatori
Aliasi	ELL3
Vanjski ID-jevi	OMIM: 609885 MGI: 2673679 HomoloGene: 11859 GeneCards: ELL3
Lokacija gena (čovjek) Hrom. Hromosom 15 (čovjek) Bend 15q15.3 Početak 43,772,605 bp Kraj 43,777,315 bp
Lokacija gena (miš) Hrom. Hromosom 2 (miš) Bend 2|2 E5 Početak 121,269,491 bp Kraj 121,274,759 bp
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine Ćelijska komponenta • Jedarce • transcription elongation factor complex • jedro • nukleoplazma Biološki proces • positive regulation of DNA-templated transcription, elongation • positive regulation of neurogenesis • positive regulation of neural precursor cell proliferation • transcription elongation from RNA polymerase II promoter • regulation of epithelial to mesenchymal transition • DNA-templated transcription, elongation • GO:0009373 regulation of transcription, DNA-templated • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator • transcription by RNA polymerase II • Spermatogeneza • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II • stem cell differentiation • transcription, DNA-templated • negative regulation of signal transduction by p53 class mediator • snRNA transcription by RNA polymerase II Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	80237	269344
Ensembl	ENSG00000128886	ENSMUSG00000027246
UniProt	Q9HB65	Q80VR2
RefSeq (mRNK)	NM_025165	NM_145973
RefSeq (bjelančevina)	NP_079441	NP_666085
Lokacija (UCSC)	Chr 15: 43.77 – 43.78 Mb	Chr 2: 121.27 – 121.27 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Međunarodni konzorcij za radijacijsko mapiranje locirao je gen ELL3 na hromosomu 15 (RH44309).

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 397 aminokiselina, a molekulska težina 45.361 Da..

10		20		30		40		50
MEELQEPLRG		QLRLCFTQAA		RTSLLLLRLN		DAALRALQEC		QRQQVRPVIA
FQGHRGYLRL		PGPGWSCLFS		FIVSQCCQEG		AGGSLDLVCQ		RFLRSGPNSL
HCLGSLRERL		IIWAAMDSIP		APSSVQGHNL		TEDARHPESW		QNTGGYSEGD
AVSQPQMALE		EVSVSDPLAS		NQGQSLPGSS		REHMAQWEVR		SQTHVPNREP
VQALPSSASR		KRLDKKRSVP		VATVELEEKR		FRTLPLVPSP		LQGLTNQDLQ
EGEDWEQEDE		DMDPRLEHSS		SVQEDSESPS		PEDIPDYLLQ		YRAIHSAEQQ
HAYEQDFETD		YAEYRILHAR		VGTASQRFIE		LGAEIKRVRR		GTPEYKVLED
KIIQEYKKFR		KQYPSYREEK		RRCEYLHQKL		SHIKGLILEF		EEKNRGS

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Pretragom baze podataka EST, Miller et al. (2000) identificirali su sekvence koje pokazuju visoku homologiju sa C-terminalnim sekvencama ELL i ELL2 . Koristeći strategiju zasnovanu na PCR-u, klonirali su ljudsku cDNA pune dužine, koju su označili kao ELL3. Izvedeni približno 400-aminokiselinski protein dijeli oko 50% identiteta sekvence s ELL i ELL2. Northern blot analizom otkriven je transkript od 2 kb eksprimiran isključivo u sjemenicima. Studije imunofluorescencije lokalizirale su ELL3 u jedru.

Funkcija gena

Miller et al. (2000) utvrdili su da ELL3, poput ELL i ELL2, može povećati katalitsku brzinu izduživanja transkripcije RNK-polimerazom II.

Dopunska literatura

• Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW (juli 2011). "Human mediator subunit MED26 functions as a docking site for transcription elongation factors". Cell. 146 (1): 92–104. doi:10.1016/j.cell.2011.06.005. PMC 3145325. PMID 21729782.
• Park G, Gong Z, Chen J, Kim JE (april 2010). "Characterization of the DOT1L network: implications of diverse roles for DOT1L". The Protein Journal. 29 (3): 213–23. doi:10.1007/s10930-010-9242-8. PMID 20431927.
• Miller T, Williams K, Johnstone RW, Shilatifard A (oktobar 2000). "Identification, cloning, expression, and biochemical characterization of the testis-specific RNA polymerase II elongation factor ELL3". The Journal of Biological Chemistry. 275 (41): 32052–6. doi:10.1074/jbc.M005175200. PMID 10882741.
• Law IK, Liu L, Xu A, Lam KS, Vanhoutte PM, Che CM, Leung PT, Wang Y (maj 2009). "Identification and characterization of proteins interacting with SIRT1 and SIRT3: implications in the anti-aging and metabolic effects of sirtuins". Proteomics. 9 (9): 2444–56. doi:10.1002/pmic.200800738. PMID 19343720.