CNOT4

Podjedinica 4 CCR4-NOT transkripcijskog kompleksa je protein koji je kod ljudi kodiran genom CNOT4.

CNOT4
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 1E4U, 1UR6
Identifikatori
Aliasi	CNOT4
Vanjski ID-jevi	OMIM: 604911 MGI: 1859026 HomoloGene: 40870 GeneCards: CNOT4
Lokacija gena (čovjek) Hrom. Hromosom 7 (čovjek) Bend 7q33 Početak 135,361,795 bp Kraj 135,510,127 bp
Lokacija gena (miš) Hrom. Hromosom 6 (miš) Bend 6|6 B1 Početak 34,999,000 bp Kraj 35,110,659 bp
Ontologija gena Molekularna funkcija • GO:0050372 aktivnost sa transferazom ubikvitina • GO:0001948, GO:0016582 vezivanje za proteine • nucleic acid binding • vezivanje iona metala • aktivnost sa transferazom • vezivanje sa RNK Ćelijska komponenta • citosol • citoplazma • CCR4-NOT complex • jedro Biološki proces • protein ubiquitination • nuclear-transcribed mRNA poly(A) tail shortening • protein autoubiquitination • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • ubiquitin-dependent protein catabolic process • regulation of megakaryocyte differentiation Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	4850	53621
Ensembl	ENSG00000080802	ENSMUSG00000038784
UniProt	O95628	Q8BT14
RefSeq (mRNK)	NM_001008225 NM_001190847 NM_001190848 NM_001190849 NM_001190850 NM_013316 NM_001393370 NM_001393371 NM_001393372 NM_001393373 NM_001393374 NM_001393375	NM_001164411 NM_001164412 NM_001164413 NM_001164414 NM_016877
RefSeq (bjelančevina)	NP_001008226 NP_001177776 NP_001177777 NP_001177778 NP_001177779 NP_037448	NP_001157883 NP_001157884 NP_001157885 NP_001157886 NP_058573
Lokacija (UCSC)	Chr 7: 135.36 – 135.51 Mb	Chr 6: 35 – 35.11 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 575 aminokiselina, а molekulska težina 63.510 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSRSPDAKED		PVECPLCMEP		LEIDDINFFP		CTCGYQICRF		CWHRIRTDEN
GLCPACRKPY		PEDPAVYKPL		SQEELQRIKN		EKKQKQNERK		QKISENRKHL
ASVRVVQKNL		VFVVGLSQRL		ADPEVLKRPE		YFGKFGKIHK		VVINNSTSYA
GSQGPSASAY		VTYIRSEDAL		RAIQCVNNVV		VDGRTLKASL		GTTKYCSYFL
KNMQCPKPDC		MYLHELGDEA		ASFTKEEMQA		GKHQEYEQKL		LQELYKLNPN
FLQLSTGSVD		KNKNKVTPLQ		RYDTPIDKPS		DSLSIGNGDN		SQQISNSDTP
SPPPGLSKSN		PVIPISSSNH		SARSPFEGAV		TESQSLFSDN		FRHPNPIPSG
LPPFPSSPQT		SSDWPTAPEP		QSLFTSETIP		VSSSTDWQAA		FGFGSSKQPE
DDLGFDPFDV		TRKALADLIE		KELSVQDQPS		LSPTSLQNSS		SHTTTAKGPG
SGFLHPAAAT		NANSLNSTFS		VLPQRFPQFQ		QHRAVYNSFS		FPGQAARYPW
MAFPRNSIMH		LNHTANPTSN		SNFLDLNLPP		QHNTGLGGIP		VAGEEEVKVS
TMPLSTSSHS		LQQGQQPTSL		HTTVA

Funkcija

Protein kodiran ovim genom podjedinica je kompleksa CCR4-NOT, globalnog transkripcijskog regulatora i deadenilazatora. Kodirani protein stupa u interakciju s CNOT1 i ima aktivnost ubikvitin-ligaza. Za ovaj gen pronađeno je nekoliko varijanti transkripta, koje kodiraju različite izoforme. (prema bRefSeq, juli 2010). Bolesti povezane sa CNOT4 uključuju adenoiditis.

Dopunska literatura

• Grönholm J, Kaustio M, Myllymäki H, Kallio J, Saarikettu J, Kronhamn J, Valanne S, Silvennoinen O, Rämet M (mart 2012). "Not4 enhances JAK/STAT pathway-dependent gene expression in Drosophila and in human cells". FASEB Journal. 26 (3): 1239–50. doi:10.1096/fj.11-195875. PMID 22159038. S2CID 25618632.
• Winkler GS, Albert TK, Dominguez C, Legtenberg YI, Boelens R, Timmers HT (mart 2004). "An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair". Journal of Molecular Biology. 337 (1): 157–65. doi:10.1016/j.jmb.2004.01.031. PMID 15001359.
• Lau NC, Kolkman A, van Schaik FM, Mulder KW, Pijnappel WW, Heck AJ, Timmers HT (septembar 2009). "Human Ccr4-Not complexes contain variable deadenylase subunits" (PDF). The Biochemical Journal. 422 (3): 443–53. doi:10.1042/BJ20090500. PMID 19558367.
• Albert TK, Hanzawa H, Legtenberg YI, de Ruwe MJ, van den Heuvel FA, Collart MA, Boelens R, Timmers HT (februar 2002). "Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex". The EMBO Journal. 21 (3): 355–64. doi:10.1093/emboj/21.3.355. PMC 125831. PMID 11823428.
• Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R (april 2004). "Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches". Structure. 12 (4): 633–44. doi:10.1016/j.str.2004.03.004. PMID 15062086.
• Wistow G, Bernstein SL, Wyatt MK, Fariss RN, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K (juni 2002). "Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants". Molecular Vision. 8: 205–20. PMID 12107410.
• Mersman DP, Du HN, Fingerman IM, South PF, Briggs SD (april 2009). "Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression". Genes & Development. 23 (8): 951–62. doi:10.1101/gad.1769209. PMC 2675863. PMID 19346402.
• Hanzawa H, de Ruwe MJ, Albert TK, van Der Vliet PC, Timmers HT, Boelens R (mart 2001). "The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers". The Journal of Biological Chemistry. 276 (13): 10185–90. doi:10.1074/jbc.M009298200. PMID 11087754.
• Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (februar 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Research. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.