CCL5
Hemokinski
(C-C motivni)
ligand 5
(CCL5) je protein koji je kod ljudi kodiran genom CCL5.[5] Poznat je i pod nazivom RANTES (reguliran aktivacijom, normalni T ćelija-eksprimirani i lučeni).
Dužina polipeptidnog lanca je 91 aminokiselina, a molekulska težina 9.990 Da.[6]
Sekvenca
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
10 | 20 | 30 | 40 | 50 | ||||
---|---|---|---|---|---|---|---|---|
MKVSAAALAV | ILIATALCAP | ASASPYSSDT | TPCCFAYIAR | PLPRAHIKEY | ||||
FYTSGKCSNP | AVVFVTRKNR | QVCANPEKKW | VREYINSLEM | S |
Funkcija
urediCCL5 je 8-kDa-ltonski protein klasificiran kao hemotaksijski citokin ili hemokin. CCL5 je hemotaksičan za T-ćelije, eozinofile i bazofile i ima aktivnu ulogu u regrutovanju leukocita na upalna mjesta. Uz pomoć određenih citokina (tj. IL-2 i IFN-γ) koje oslobađaju T-ćelije , CCL5 također inducira proliferaciju i aktivaciju određenih ćelija prirodnog ubica (NK) da bi se stvorile CHAK ćelije (CC-hemokinski-aktivirane ubice).[7] Također je supresivni faktor HIV-a koji se oslobađa iz CD8 + T ćelija.[8] Ovaj hemokinski gen je kod ljudi lokaliziran na hromosomu 17.[5]
RANTES je prvi put identificiran u potrazi za genima eksprimiranim kao "kasno", (3-5 dana) nakon aktivacije T-ćelija. Naknadno je utvrđeno da je to CC hemokin i eksprimiran u više od 100 ljudskih bolesti. Ekspresija RANTES-a u T-limfocitima je regulirana Kruppelovim faktorom 13 (KLF13).[9][10][11][12] RANTES je, zajedno sa srodnim hemokinima MIP-1alfa i MIP-1beta, identificiran kao prirodni HIV-supresivni faktor kojeg luče aktivirane CD8 + T-ćelije i druge imunske ćelije.[8] Nedavno je protein RANTES dizajniran za proizvodnju in vivo kod bakterija Lactobacillus, a ovo rešenje se razvija u mogući lokalni mikrobicid koji inhibira ulazak HIV-a.[13]
Interakcije
urediPokazano je da CCL5 komunicira sa CCR3,[14][15] CCR5[15][16][17][18] i CCR1.[15][17]
CCL5 također aktivira receptor povezan sa G-proteinima GPR75.[19]
Također pogledajte
urediReference
uredi
- ^ a b c ENSG00000274233 GRCh38: Ensembl release 89: ENSG00000271503, ENSG00000274233 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000035042 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b Donlon TA, Krensky AM, Wallace MR, Collins FS, Lovett M, Clayberger C (mart 1990). "Localization of a human T-cell-specific gene, RANTES (D17S136E), to chromosome 17q11.2-q12" (PDF). Genomics. 6 (3): 548–53. doi:10.1016/0888-7543(90)90485-D. hdl:2027.42/28717. PMID 1691736.
- ^ "UniProt, P13501". Pristupljeno 27. 6. 2021.
- ^ Maghazachi AA, Al-Aoukaty A, Schall TJ (februar 1996). "CC chemokines induce the generation of killer cells from CD56+ cells". Eur. J. Immunol. 26 (2): 315–9. doi:10.1002/eji.1830260207. PMID 8617297.
- ^ a b Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P (decembar 1995). "Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells". Science. 270 (5243): 1811–5. doi:10.1126/science.270.5243.1811. PMID 8525373. S2CID 84062618.
- ^ Schall TJ, Jongstra J, Dyer BJ, Jorgensen J, Clayberger C, Davis MM, Krensky AM (august 1988). "A human T cell-specific molecule is a member of a new gene family". J. Immunol. 141 (3): 1018–25. PMID 2456327.
- ^ Alan M. Krensky (1995). Biology of the Chemokine in Rantes (Molecular Biology Intelligence Unit). R G Landes Co. ISBN 978-1-57059-253-9.
- ^ Song A, Chen YF, Thamatrakoln K, Storm TA, Krensky AM (januar 1999). "RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes". Immunity. 10 (1): 93–103. doi:10.1016/S1074-7613(00)80010-2. PMID 10023774.
- ^ Song A, Nikolcheva T, Krensky AM (oktobar 2000). "Transcriptional regulation of RANTES expression in T lymphocytes". Immunol. Rev. 177: 236–45. doi:10.1034/j.1600-065X.2000.17610.x. PMID 11138780. S2CID 30184294.
- ^ Vangelista L, Secchi M, Liu X, Bachi A, Jia L, Xu Q, Lusso P (juli 2010). "Engineering of Lactobacillus jensenii to secrete RANTES and a CCR5 antagonist analogue as live HIV-1 blockers". Antimicrob. Agents Chemother. 54 (7): 2994–3001. doi:10.1128/AAC.01492-09. PMC 2897324. PMID 20479208. Sažetak – Science Daily.
- ^ Daugherty BL, Siciliano SJ, DeMartino JA, Malkowitz L, Sirotina A, Springer MS (maj 1996). "Cloning, expression, and characterization of the human eosinophil eotaxin receptor". J. Exp. Med. 183 (5): 2349–54. doi:10.1084/jem.183.5.2349. PMC 2192548. PMID 8642344.
- ^ a b c Struyf S, Menten P, Lenaerts JP, Put W, D'Haese A, De Clercq E, Schols D, Proost P, Van Damme J (juli 2001). "Diverging binding capacities of natural LD78beta isoforms of macrophage inflammatory protein-1alpha to the CC chemokine receptors 1, 3 and 5 affect their anti-HIV-1 activity and chemotactic potencies for neutrophils and eosinophils". Eur. J. Immunol. 31 (7): 2170–8. doi:10.1002/1521-4141(200107)31:7<2170::AID-IMMU2170>3.0.CO;2-D. PMID 11449371.
- ^ Slimani H, Charnaux N, Mbemba E, Saffar L, Vassy R, Vita C, Gattegno L (oktobar 2003). "Interaction of RANTES with syndecan-1 and syndecan-4 expressed by human primary macrophages". Biochim. Biophys. Acta. 1617 (1–2): 80–8. doi:10.1016/j.bbamem.2003.09.006. PMID 14637022.
- ^ a b Proudfoot AE, Fritchley S, Borlat F, Shaw JP, Vilbois F, Zwahlen C, Trkola A, Marchant D, Clapham PR, Wells TN (april 2001). "The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity". J. Biol. Chem. 276 (14): 10620–6. doi:10.1074/jbc.M010867200. PMID 11116158.
- ^ Laplana M, Fibla J (april 2012). "Distribution of functional polymorphic variants of inflammation-related genes RANTES and CCR5 in long-lived individuals". Cytokine. 58 (1): 10–13. doi:10.1016/j.cyto.2011.12.021. PMID 22265023.
- ^ Ignatov A, Robert J, Gregory-Evans C, Schaller HC (novembar 2006). "RANTES stimulates Ca2+ mobilization and inositol trisphosphate (IP3) formation in cells transfected with G protein-coupled receptor 75". Br. J. Pharmacol. 149 (5): 490–7. doi:10.1038/sj.bjp.0706909. PMC 2014681. PMID 17001303.
Dopunska literatura
uredi
- Muthumani K, Desai BM, Hwang DS, Choo AY, Laddy DJ, Thieu KP, Rao RG, Weiner DB (2004). "HIV-1 Vpr and anti-inflammatory activity". DNA Cell Biol. 23 (4): 239–47. doi:10.1089/104454904773819824. PMID 15142381.
- Zhao RY, Elder RT (2005). "Viral infections and cell cycle G2/M regulation". Cell Res. 15 (3): 143–9. doi:10.1038/sj.cr.7290279. PMID 15780175.
- Zhao RY, Bukrinsky M, Elder RT (2005). "HIV-1 viral protein R (Vpr) & host cellular responses". Indian J. Med. Res. 121 (4): 270–86. PMID 15817944.
- Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Res. 15 (11–12): 923–34. doi:10.1038/sj.cr.7290370. PMID 16354571.
- Ignatov A, Robert J, Gregory-Evans C, Schaller HC (Nov 2006). "RANTES stimulates Ca2+ mobilization and inositol trisphosphate (IP3) formation in cells transfected with G protein-coupled receptor 75". Br J Pharmacol. 149 (5): 490–7. doi:10.1038/sj.bjp.0706909. PMC 2014681. PMID 17001303.
Vanjski linkovi
uredi- Lokacija ljudskog genoma CCL5 i stranica sa detaljima o genu CCL5 u UCSC Genome Browseru.
- P13501