TPA

Tonsokuiliki protein popravljanja oštećene DNK je protein koji je kod ljudi kodiran genom TONSL.

TPA
Identifikatori
Aliasi
Vanjski ID-jevi	GeneCards: [1]
Ortolozi
Vrste	Čovjek	Miš
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n a	n/a
RefSeq (mRNK)	n/a	n/a
RefSeq (bjelančevina)	n/a	n/a
Lokacija (UCSC)	n/a	n/a
PubMed pretraga	n/a	n/a
Wikipodaci
Pogledaj/uredi – čovjek

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 1.378 aminokiselina, а molekulska težina 150.929 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSLERELRQL		SKAKAKAQRA		GQRREEAALC		HQLGELLAGH		GRYAEALEQH
WQELQLRERA		DDPLGCAVAH		RKIGERLAEM		EDYPAALQHQ		HQYLELAHSL
RNHTELQRAW		ATIGRTHLDI		YDHCQSRDAL		LQAQAAFEKS		LAIVDEELEG
TLAQGELNEM		RTRLYLNLGL		TFESLQQTAL		CNDYFRKSIF		LAEQNHLYED
LFRARYNLGT		IHWRAGQHSQ		AMRCLEGARE		CAHTMRKRFM		ESECCVVIAQ
VLQDLGDFLA		AKRALKKAYR		LGSQKPVQRA		AICQNLQHVL		AVVRLQQQLE
EAEGRDPQGA		MVICEQLGDL		FSKAGDFPRA		AEAYQKQLRF		AELLDRPGAE
RAIIHVSLAT		TLGDMKDHHG		AVRHYEEELR		LRSGNVLEEA		KTWLNIALSR
EEAGDAYELL		APCFQKALSC		AQQAQRPQLQ		RQVLQHLHTV		QLRLQPQEAP
ETETRLRELS		VAEDEDEEEE		AEEAAATAES		EALEAGEVEL		SEGEDDTDGL
TPQLEEDEEL		QGHLGRRKGS		KWNRRNDMGE		TLLHRACIEG		QLRRVQDLVR
QGHPLNPRDY		CGWTPLHEAC		NYGHLEIVRF		LLDHGAAVDD		PGGQGCEGIT
PLHDALNCGH		FEVAELLLER		GASVTLRTRK		GLSPLETLQQ		WVKLYRRDLD
LETRQKARAM		EMLLQAAASG		QDPHSSQAFH		TPSSLLFDPE		TSPPLSPCPE
PPSNSTRLPE		ASQAHVRVSP		GQAAPAMARP		RRSRHGPASS		SSSSEGEDSA
GPARPSQKRP		RCSATAQRVA		AWTPGPASNR		EAATASTSRA		AYQAAIRGVG
SAQSRLGPGP		PRGHSKALAP		QAALIPEEEC		LAGDWLELDM		PLTRSRRPRP
RGTGDNRRPS		STSGSDSEES		RPRARAKQVR		LTCMQSCSAP		VNAGPSSLAS
EPPGSPSTPR		VSEPSGDSSA		AGQPLGPAPP		PPIRVRVQVQ		DHLFLIPVPH
SSDTHSVAWL		AEQAAQRYYQ		TCGLLPRLTL		RKEGALLAPQ		DLIPDVLQSN
DEVLAEVTSW		DLPPLTDRYR		RACQSLGQGE		HQQVLQAVEL		QGLGLSFSAC
SLALDQAQLT		PLLRALKLHT		ALRELRLAGN		RLGDKCVAEL		VAALGTMPSL
ALLDLSSNHL		GPEGLRQLAM		GLPGQATLQS		LEELDLSMNP		LGDGCGQSLA
SLLHACPLLS		TLRLQACGFG		PSFFLSHQTA		LGSAFQDAEH		LKTLSLSYNA
LGAPALARTL		QSLPAGTLLH		LELSSVAAGK		GDSDLMEPVF		RYLAKEGCAL
AHLTLSANHL		GDKAVRDLCR		CLSLCPSLIS		LDLSANPEIS		CASLEELLST
LQKRPQGLSF		LGLSGCAVQG		PLGLGLWDKI		AAQLRELQLC		SRRLCAEDRD
ALRQLQPSRP		GPGECTLDHG		SKLFFRRL

Funkcija

Smatra se da je protein kodiran ovim genom negativan regulator NF-kappa-B posredovane transkripcije. Kodirani protein može vezati komplekse NF-kappa-B i zarobiti ih u citoplazmi, sprječavajući ih da uđu u jedro i stupe u interakciju s DNK. Fosforilacija ovog proteina cilja ga na razgradnju putem ubikvitinacije, što oslobađa komplekse NF-kappa-B da uđu u ćelijsko jedro.

Dopunska literatura

• Ask K, Jasencakova Z, Menard P, Feng Y, Almouzni G, Groth A (april 2012). "Codanin-1, mutated in the anaemic disease CDAI, regulates Asf1 function in S-phase histone supply". The EMBO Journal. 31 (8): 2013–23. doi:10.1038/emboj.2012.55. PMC 3343338. PMID 22407294.
• Norman DA, Barton PJ (januar 2000). "Isolation, sequence, and chromosomal localisation of the human IkappaBR gene (NFKBIL2)". Annals of Human Genetics. 64 (Pt 1): 15–23. doi:10.1046/j.1469-1809.2000.6410015.x. PMID 11246458. S2CID 83963944.
• Duro E, Lundin C, Ask K, Sanchez-Pulido L, MacArtney TJ, Toth R, Ponting CP, Groth A, Helleday T, Rouse J (novembar 2010). "Identification of the MMS22L-TONSL complex that promotes homologous recombination". Molecular Cell. 40 (4): 632–44. doi:10.1016/j.molcel.2010.10.023. PMID 21055984.
• O'Connell BC, Adamson B, Lydeard JR, Sowa ME, Ciccia A, Bredemeyer AL, Schlabach M, Gygi SP, Elledge SJ, Harper JW (novembar 2010). "A genome-wide camptothecin sensitivity screen identifies a mammalian MMS22L-NFKBIL2 complex required for genomic stability". Molecular Cell. 40 (4): 645–57. doi:10.1016/j.molcel.2010.10.022. PMC 3006237. PMID 21055985.
• O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, Durocher D (novembar 2010). "The MMS22L-TONSL complex mediates recovery from replication stress and homologous recombination". Molecular Cell. 40 (4): 619–31. doi:10.1016/j.molcel.2010.10.024. PMC 3031522. PMID 21055983.
• Chapman SJ, Khor CC, Vannberg FO, Rautanen A, Walley A, Segal S, Moore CE, Davies RJ, Day NP, Peshu N, Crook DW, Berkley JA, Williams TN, Scott JA, Hill AV (2010). "Common NFKBIL2 polymorphisms and susceptibility to pneumococcal disease: a genetic association study". Critical Care. 14 (6): R227. doi:10.1186/cc9377. PMC 3220025. PMID 21171993.
• Ray P, Zhang DH, Elias JA, Ray A (maj 1995). "Cloning of a differentially expressed I kappa B-related protein". The Journal of Biological Chemistry. 270 (18): 10680–5. doi:10.1074/jbc.270.18.10680. PMID 7738005.
• Piwko W, Olma MH, Held M, Bianco JN, Pedrioli PG, Hofmann K, Pasero P, Gerlich DW, Peter M (decembar 2010). "RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells". The EMBO Journal. 29 (24): 4210–22. doi:10.1038/emboj.2010.304. PMC 3018799. PMID 21113133.