SPAG8

SPAG8
Identifikatori
Aliasi	SPAG8
Vanjski ID-jevi	OMIM: 605731 MGI: 3056295 HomoloGene: 8250 GeneCards: SPAG8
Lokacija gena (čovjek)
Hrom.	Hromosom 9 (čovjek)
Kraj	35,812,272 bp
Lokacija gena (miš)
Hrom.	Hromosom 4 (miš)
Kraj	43,653,594 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine; • molekularna funkcija; • microtubule binding;
Ćelijska komponenta	• Akrozom; • membrana; • GO:0016023 citoplazmatska vezikula; • citoskelet; • citoplazma; • jedro; • Diobeno vreteno;
Biološki proces	• single fertilization; • Ćelijska diferencijacija; • Spermatogeneza; • ćelijski ciklus; • GO:0022610 biološki proces; • GO:0003257, GO:0010735, GO:1901228, GO:1900622, GO:1904488 positive regulation of transcription by RNA polymerase II;
	Izvori:Amigo / QuickGO
Ortolozi
	26206
	433700
	ENSG00000137098
	ENSMUSG00000066196
	Q99932
	Q3V0Q6
	NM_001039592; NM_012436; NM_172312
	NM_001007463; NM_001290462
	NP_001034681; NP_758516; NP_001353689
	NP_001007464; NP_001277391
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Spermno-pridruženi antigen 8 je protein koji je kod ljudi kodiran genom SPAG8.^[5]^[6]^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 426 aminokiselina, а molekulska težina 44.819 Da.^[8]

10	20	30	40	50
METNESTEGS	RSRSRSLDIQ	PSSEGLGPTS	EPFPSSDDSP	RSALAAATAA
AAAAASAAAA	TAAFTTAKAA	ALSTKTPAPC	SEFMEPSSDP	SLLGEPCAGP
GFTHNIAHGS	LGFEPVYVSC	IAQDTCTTTD	HSSNPGPVPG	SSSGPVLGSS
SGAGHGSGSG	SGPGCGSVPG	SGSGPGPGSG	PGSGPGHGSG	SHPGPASGPG
PDTGPDSELS	PCIPPGFRNL	VADRVPNYTS	WSQHCPWEPQ	KQPPWEFLQV
LEPGARGLWK	PPDIKGKLMV	CYETLPRGQC	LLYNWEEERA	TNHLDQVPSM
QDGSESFFFR	HGHRGLLTMQ	LKSPMPSSTT	QKDSYQPPGN	VYWPLRGKRE
AMLEMLLQHQ	ICKEVQAEQE	PTRKLFEVES	VTHHDYRMEL	AQAGTPAPTK
PHDYRQEQPE	TFWIQRAPQL	PVCEGD

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

Funkcija

Povezanost antitijela protiv sperme sa slučajevima neobjašnjive neplodnosti implicira ulogu ovih antitijela u blokiranju oplodnje. Poboljšana dijagnoza i liječenje imunske neplodnosti, kao i identifikacija proteina za ciljanu kontracepciju, ovise o identifikaciji i karakterizaciji relevantnih antigena sperme. Protein kodiran ovim genom prepoznaju spermatozoidi koji aglutiraju antitijela neplodne žene. Ovaj protein je lokaliziran u zametnim ćelijamama sjemenika u svim fazama spermatogeneze i lokaliziran je u akrosomskom području zrelih spermatozoida. Oposane su alternativno prerađene varijante koje kodiraju različite izoforme proteina, ali su određene samo dvije sekvence cijele dužine.^[7]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000137098 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000066196 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Wang H, Miao S, Chen D, Wang L, Koide SS (Nov 1999). "Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a human sperm membrane protein (hSMP-1)". Biochim Biophys Acta. 1447 (1): 119–24. doi:10.1016/s0167-4781(99)00136-0. PMID 10500252.
^ Liu QY, Wang LF, Miao SY, Catterall JF (Oct 1996). "Expression and characterization of a novel human sperm membrane protein". Biol Reprod. 54 (2): 323–30. doi:10.1095/biolreprod54.2.323. PMID 8788182.
^ ^a ^b "Entrez Gene: SPAG8 sperm associated antigen 8".
^ "UniProt, Q99932". Pristupljeno 11. 9. 2021.

Dopunska literatura

Diekman AB, Herr JC (1997). "Sperm antigens and their use in the development of an immunocontraceptive". Am. J. Reprod. Immunol. 37 (1): 111–7. doi:10.1111/j.1600-0897.1997.tb00199.x. PMID 9138444. S2CID 39710127.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Kuang Y, Yan YC, Gao AW, et al. (2001). "Immune responses in rats following oral immunization with attenuated Salmonella typhimurium expressing human sperm antigen". Arch. Androl. 45 (3): 169–80. doi:10.1080/01485010050193940. PMID 11111865.
Zhang XD, Miao SY, Wang LF, et al. (2001). "Human sperm membrane protein (hSMP-1): a developmental testis-specific component during germ cell differentiation". Arch. Androl. 45 (3): 239–46. doi:10.1080/01485010050194020. PMID 11111873.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Tang X, Zhang J, Cai Y, et al. (2004). "Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-organizing centre". J. Mol. Med. 82 (6): 383–8. doi:10.1007/s00109-004-0535-2. PMID 15014887. S2CID 21455758.
Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9". Nature. 429 (6990): 369–74. Bibcode:2004Natur.429..369H. doi:10.1038/nature02465. PMC 2734081. PMID 15164053.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Cheng GY, Shi JL, Wang M, et al. (2007). "Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody". Asian J. Androl. 9 (1): 23–9. doi:10.1111/j.1745-7262.2007.00247.x. PMID 17187156.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000137098 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000066196 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10500252-5] Wang H, Miao S, Chen D, Wang L, Koide SS (Nov 1999). "Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a human sperm membrane protein (hSMP-1)". Biochim Biophys Acta. 1447 (1): 119–24. doi:10.1016/s0167-4781(99)00136-0. PMID 10500252.

[pmid8788182-6] Liu QY, Wang LF, Miao SY, Catterall JF (Oct 1996). "Expression and characterization of a novel human sperm membrane protein". Biol Reprod. 54 (2): 323–30. doi:10.1095/biolreprod54.2.323. PMID 8788182.

[entrez-7] "Entrez Gene: SPAG8 sperm associated antigen 8".

[UniProt-8] "UniProt, Q99932". Pristupljeno 11. 9. 2021.

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