PURB

Transkripcijski aktivatorski protein Pur-beta je protein koji je kod ljudi kodiran genom PURB.

PURB
Identifikatori
Aliasi	PURB
Vanjski ID-jevi	OMIM: 608887 MGI: 1338779 HomoloGene: 69087 GeneCards: PURB
Lokacija gena (čovjek) Hrom. Hromosom 7 (čovjek) Bend 7p13 Početak 44,876,299 bp Kraj 44,885,530 bp
Lokacija gena (miš) Hrom. Hromosom 11 (miš) Bend 11 A1|11 4.16 cM Početak 6,417,599 bp Kraj 6,425,917 bp
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • RNA polymerase II transcription regulatory region sequence-specific DNA binding • GO:0001078, GO:0001214, GO:0001206 DNA-binding transcription repressor activity, RNA polymerase II-specific • vezivanje sa DNK • double-stranded DNA binding • SMAD binding • single-stranded DNA binding • transcription factor binding • mRNA binding • vezivanje sa RNK • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific • purine-rich negative regulatory element binding • sequence-specific DNA binding Ćelijska komponenta • Replikacijski protein A • jedro Biološki proces • regulation of myeloid cell differentiation • GO:0009373 regulation of transcription, DNA-templated • GO:1901227 negative regulation of transcription by RNA polymerase II • Ćelijska proliferacija • Ćelijska diferencijacija • transcription, DNA-templated • GO:0045996 negative regulation of transcription, DNA-templated Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	5814	19291
Ensembl	ENSG00000146676	ENSMUSG00000094483
UniProt	Q96QR8	O35295
RefSeq (mRNK)	NM_033224	NM_011221
RefSeq (bjelančevina)	NP_150093	NP_035351
Lokacija (UCSC)	Chr 7: 44.88 – 44.89 Mb	Chr 11: 6.42 – 6.43 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 312 aminokiselina, а molekulska težina 33.241 Da.

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MADGDSGSER		GGGGGPCGFQ		PASRGGGEQE		TQELASKRLD		IQNKRFYLDV
KQNAKGRFLK		IAEVGAGGSK		SRLTLSMAVA		AEFRDSLGDF		IEHYAQLGPS
SPEQLAAGAE		EGGGPRRALK		SEFLVRENRK		YYLDLKENQR		GRFLRIRQTV
NRGGGGFGAG		PGPGGLQSGQ		TIALPAQGLI		EFRDALAKLI		DDYGGEDDEL
AGGPGGGAGG		PGGGLYGELP		EGTSITVDSK		RFFFDVGCNK		YGVFLRVSEV
KPSYRNAITV		PFKAWGKFGG		AFCRYADEMK		EIQERQRDKL		YERRGGGSGG
GEESEGEEVD		ED

Funkcija

Ovaj genski proizvod je sekvencnospecifični protein koji se vezuje za jednolančanu sekvencu DNK. Preferirano se veže za jedan lanac purin-bogatog elementa zvanog PUR, koji je prisutan na početku replikacije i u regijama koje okružuju gene u raznim eukariotima, od kvasaca do ljudi.

Stoga je uključen u kontrolu i replikacije i transkripcije DNK. Delecija ovog gena povezana je s mijelodisplazijskim sindromom i akutnom mijelogenom leukemijom.

Dopunska literatura

• Sanger Centre, The; Washington University Genome Sequencing Cente, The (1999). "Toward a complete human genome sequence". Genome Res. 8 (11): 1097–108. doi:10.1101/gr.8.11.1097. PMID 9847074.
• Kelm RJ, Cogan JG, Elder PK, et al. (1999). "Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter". J. Biol. Chem. 274 (20): 14238–45. doi:10.1074/jbc.274.20.14238. PMID 10318844.
• Lezon-Geyda K, Najfeld V, Johnson EM (2001). "Deletions of PURA, at 5q31, and PURB, at 7p13, in myelodysplastic syndrome and progression to acute myelogenous leukemia". Leukemia. 15 (6): 954–62. doi:10.1038/sj.leu.2402108. PMID 11417483.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human Chromosome 7: DNA Sequence and Biology". Science. 300 (5620): 767–72. Bibcode:2003Sci...300..767S. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205.
• Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–64. Bibcode:2003Natur.424..157H. doi:10.1038/nature01782. PMID 12853948.
• Gupta M, Sueblinvong V, Raman J, et al. (2004). "Single-stranded DNA-binding proteins PURalpha and PURbeta bind to a purine-rich negative regulatory element of the alpha-myosin heavy chain gene and control transcriptional and translational regulation of the gene expression. Implications in the repression of alpha-myosin heavy chain during heart failure". J. Biol. Chem. 278 (45): 44935–48. doi:10.1074/jbc.M307696200. PMID 12933792.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.