PDCD5

PDCD5
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1YYB, 2CRU, 2K6B
Identifikatori
Aliasi	PDCD5, TFAR19, protein 5 programirane ćelijske smrti
Vanjski ID-jevi	OMIM: 604583 MGI: 3782009 HomoloGene: 10506 GeneCards: PDCD5
Lokacija gena (čovjek)
Hrom.	Hromosom 19 (čovjek)
Kraj	32,587,453 bp
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • heparin binding; • GO:0001948, GO:0016582 vezivanje za proteine; • acetyltransferase activator activity; • beta-tubulin binding;
Ćelijska komponenta	• citoplazma; • Egzosom; • jedro; • citosol;
Biološki proces	• cellular response to transforming growth factor beta stimulus; • negative regulation of chaperone-mediated protein folding; • positive regulation of release of cytochrome c from mitochondria; • GO:0097285 apoptoza; • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; • GO:1901313 positive regulation of gene expression; • positive regulation of protein insertion into mitochondrial outer membrane; • negative regulation of cell population proliferation; • positive regulation of apoptotic process;
	Izvori:Amigo / QuickGO
Ortolozi
	9141
	100042424
	ENSG00000105185
	n/a
	O14737
	P56812
	NM_004708
	XM_001478256
	NP_004699
	NP_062720
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Prtein 5 programirane ćelijske smrti je protein koji jr kod ljudi kodiran genom PDCD5.^[4]^[5]

Ovaj gen kodira protein sa ekspresijom u tumorskim ćelijama tokom apoptoze, neovisno od stimulusa koji je induciraju. Prije indukcije apoptoze, ovaj genski proizvod distribuira se i u jedru i citoplazmi.

Kada se inducira apoptoza, nivo ovog proteina raste i prelaskom iz citoplazme akumulira se u jedru. Iako njegova tačna funkcija nije definirana, smatra se da ovaj protein ima ranu i univerzalnu ulogu u apoptozi.^[5]

Kloniranje i ekspresija

Koristeći analizu razlika predstavnika cDNK, Liu et al. (1999) identificirali su novi gen, označen kao TFAR19, iz ćelija TF1 (ljudske premeloidne ćelijske linije, uspostavljene od pacijenta sa eritroleukemijom) koje su podvrgnute apoptozi, nakon povlačenja GM-CSF iz medija za kulturu ćelija. Gen TFAR19 kodira protein od 125 aminokiselina, sa ATG inicijacijskim kodonom na nukleotidima 25-27. Sadrži mjesto fosforilacije protein-kinaze ovisne o cAMP-u i cGMP-u, više mjesta fosforilacije protein-kinaze C i mjesto fosforilacije kazein-kinaze II. Dijeli značajnu homologiju s odgovarajućim proteinima nekih vrsta, od kvasaca do miševa. Predviđa se je protein TFAR19 lokaliziran u jedru. Analiza točkastih obojenja pokazala je da se TFAR19 sveprisutno eksprimira, ali je najistaknutiji u srcu, sjemenici, bubrezima, hipofizi, [[nadbubrežna žlijezda|nadbubrežnoj žlijezdi i posteljici. Ekspresija u fetusnim tkivima je znatno niža od ekspresije u tkivima odraslih. Ekspresija TFAR19 je pojačano regulirana u ćelijama tumora koje su u apoptozi i pojačava apoptozu izazvanu faktorom rasta ili lišavanjem seruma.^[6]^[7]

Aminokiselinski sastav

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MADEELEALR	RQRLAELQAK	HGDPGDAAQQ	EAKHREAEMR	NSILAQVLDQ
SARARLSNLA	LVKPEKTKAV	ENYLIQMARY	GQLSEKVSEQ	GLIEILKKVS
QQTEKTTTVK	FNRRKVMDSD	EDDDY

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000105185 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Liu H, Wang Y, Zhang Y, Song Q, Di C, Chen G, Tang J, Ma D (Feb 1999). "TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal". Biochem Biophys Res Commun. 254 (1): 203–10. doi:10.1006/bbrc.1998.9893. PMID 9920759.
^ ^a ^b "Entrez Gene: PDCD5 programmed cell death 5".
^ Lisitsyn, N., Lisitsyn, N., Wigler, M. Cloning the differences between two complex genomes. Science 259: 946-951, 1993. [PubMed]: 8438152
^ Liu, H., Wang, Y., Zhang, Y., Song, Q., Di, C., Chen, G., Tang, J., Ma, D. TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem. Biophys. Res. Commun. 254: 203-210, 1999. PubMed: 9920759.

Dopunska literatura

Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Chen Y, Sun R, Han W, et al. (2002). "Nuclear translocation of PDCD5 (TFAR19): an early signal for apoptosis?". FEBS Lett. 509 (2): 191–6. doi:10.1016/S0014-5793(01)03062-9. PMID 11741587. S2CID 26547410.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
Xu M, Cheng N, Gui L, et al. (2004). "The 5'-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide". Gene. 329: 39–49. doi:10.1016/j.gene.2003.12.025. PMID 15033527.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Liu D, Yao H, Chen Y, et al. (2006). "The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable α-helix having unique electrostatic potential character". Biochem. J. 392 (Pt 1): 47–54. doi:10.1042/BJ20050688. PMC 1317663. PMID 16083422.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.
Ma X, Ruan G, Wang Y, et al. (2006). "Two single-nucleotide polymorphisms with linkage disequilibrium in the human programmed cell death 5 gene 5' regulatory region affect promoter activity and the susceptibility of chronic myelogenous leukemia in Chinese population". Clin. Cancer Res. 11 (24 Pt 1): 8592–9. doi:10.1158/1078-0432.CCR-05-0039. PMID 16361542.
Yang YH, Zhao M, Li WM, et al. (2007). "Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells". Apoptosis. 11 (6): 993–1001. doi:10.1007/s10495-006-6714-6. PMID 16547588. S2CID 21178139.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Wang N, Lu HS, Guan ZP, et al. (2007). "Involvement of PDCD5 in the regulation of apoptosis in fibroblast-like synoviocytes of rheumatoid arthritis". Apoptosis. 12 (8): 1433–41. doi:10.1007/s10495-007-0070-z. PMID 17468978. S2CID 21175365.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000105185 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[3] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9920759-4] Liu H, Wang Y, Zhang Y, Song Q, Di C, Chen G, Tang J, Ma D (Feb 1999). "TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal". Biochem Biophys Res Commun. 254 (1): 203–10. doi:10.1006/bbrc.1998.9893. PMID 9920759.

[entrez-5] "Entrez Gene: PDCD5 programmed cell death 5".

[6] Lisitsyn, N., Lisitsyn, N., Wigler, M. Cloning the differences between two complex genomes. Science 259: 946-951, 1993. [PubMed]: 8438152

[7] Liu, H., Wang, Y., Zhang, Y., Song, Q., Di, C., Chen, G., Tang, J., Ma, D. TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem. Biophys. Res. Commun. 254: 203-210, 1999. PubMed: 9920759.

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