MYBBP1A
Myb-vezujući protein 1A je protein koji je kod ljudi kodiran genom MYBBP1A.[5][6]
Dužina polipeptidnog lanca je 1.328 aminokiselina, sa molekulskom težinom od 148.855.[7].
Aminokiselinska sekvenca
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MESRDPAQPM | SPGEATQSGA | RPADRYGLLK | HSREFLDFFW | DIAKPEQETR | ||||
| LAATEKLLEY | LRGRPKGSEM | KYALKRLITG | LGVGRETARP | CYSLALAQLL | ||||
| QSFEDLPLCS | ILQQIQEKYD | LHQVKKAMLR | PALFANLFGV | LALFQSGRLV | ||||
| KDQEALMKSV | KLLQALAQYQ | NHLQEQPRKA | LVDILSEVSK | ATLQEILPEV | ||||
| LKADLNIILS | SPEQLELFLL | AQQKVPSKLK | KLVGSVNLFS | DENVPRLVNV | ||||
| LKMAASSVKK | DRKLPAIALD | LLRLALKEDK | FPRFWKEVVE | QGLLKMQFWP | ||||
| ASYLCFRLLG | AALPLLTKEQ | LHLVMQGDVI | RHYGEHVCTA | KLPKQFKFAP | ||||
| EMDDYVGTFL | EGCQDDPERQ | LAVLVAFSSV | TNQGLPVTPT | FWRVVRFLSP | ||||
| PALQGYVAWL | RAMFLQPDLD | SLVDFSTNNQ | KKAQDSSLHM | PERAVFRLRK | ||||
| WIIFRLVSIV | DSLHLEMEEA | LTEQVARFCL | FHSFFVTKKP | TSQIPETKHP | ||||
| FSFPLENQAR | EAVSSAFFSL | LQTLSTQFKQ | APGQTQGGQP | WTYHLVQFAD | ||||
| LLLNHSHNVT | TVTPFTAQQR | QAWDRMLQTL | KELEAHSAEA | RAAAFQHLLL | ||||
| LVGIHLLKSP | AESCDLLGDI | QTCIRKSLGE | KPRRSRTKTI | DPQEPPWVEV | ||||
| LVEILLALLA | QPSHLMRQVA | RSVFGHICSH | LTPRALQLIL | DVLNPETSED | ||||
| ENDRVVVTDD | SDERRLKGAE | DKSEEGEDNR | SSESEEESEG | EESEEEERDG | ||||
| DVDQGFREQL | MTVLQAGKAL | GGEDSENEEE | LGDEAMMALD | QSLASLFAEQ | ||||
| KLRIQARRDE | KNKLQKEKAL | RRDFQIRVLD | LVEVLVTKQP | ENALVLELLE | ||||
| PLLSIIRRSL | RSSSSKQEQD | LLHKTARIFT | HHLCRARRYC | HDLGERAGAL | ||||
| HAQVERLVQQ | AGRQPDSPTA | LYHFNASLYL | LRVLKGNTAE | GCVHETQEKQ | ||||
| KAGTDPSHMP | TGPQAASCLD | LNLVTRVYST | ALSSFLTKRN | SPLTVPMFLS | ||||
| LFSRHPVLCQ | SLLPILVQHI | TGPVRPRHQA | CLLLQKTLSM | REVRSCFEDP | ||||
| EWKQLMGQVL | AKVTENLRVL | GEAQTKAQHQ | QALSSLELLN | VLFRTCKHEK | ||||
| LTLDLTVLLG | VLQGQQQSLQ | QGAHSTGSSR | LHDLYWQAMK | TLGVQRPKLE | ||||
| KKDAKEIPSA | TQSPISKKRK | KKGFLPETKK | RKKRKSEDGT | PAEDGTPAAT | ||||
| GGSQPPSMGR | KKRNRTKAKV | PAQANGTPTT | KSPAPGAPTR | SPSTPAKSPK | ||||
| LQKKNQKPSQ | VNGAPGSPTE | PAGQKQHQKA | LPKKGVLGKS | PLSALARKKA | ||||
| RLSLVIRSPS | LLQSGAKKKA | QVRKAGKP |
Kloniranje i ekspresija uredi
Pregledavanjem ljudske genomske biblioteke mišje cDNK Mybbp1a, Keough et al. (1999) izolirali su dio ljudskog gena MYBBP1A, koji su također nazvali P160. Koristeći genomsku sekvencu, klonirali su ljudsku cDNK MYBBP1A pune dužine. Predviđeni 1.328-aminokiselinski MYBBP1A sadrži centralno kiselo područje, ponavljanja C-terminalnih baznih aminokiselina i sekvencu potencijalnih motiva leucinom nabijenih domena u proteinu. Ljudski MYBBP1A dijeli približno 80% sličnosti aminokiselinskih sekvenci s mišjim i miševima Mybbp1a. Northern blot analizom otkriven je približno 4,5 kb MYBBP1A transkript u svim ispitivanim ljudskim linijama, a to su epiteli bubrega, eritroleukemijski karcinomi i grlića maternice, te ćelijske linije fibroblasta. Otkrili su da se ljudski EST koji odgovaraju MYBBP1A eksprimiraju u širokom spektru tkiva, što sugerira da se MYBBP1A eksprimira sveprisutno.[8]
Funkcija gena uredi
Protoonkogen MYB pretežno se eksprimira u nezrelim hematopoetskim ćelijama, gdje ima važnu ulogu u proliferaciji i diferencijaciji hemopoetskih ćelija. Onkogeno aktivirani oblici MYB su obično imaju N– i / ili C-terminalne delecije normalnog MYB proteina. Uklanjanje C-kraja MYB remeti ili briše regiju nazvanu negativni regulatorni domen (NRD), što rezultira povećanjem vezivanja, transaktivacije i transformacije DNK od pomoću MYB-a. Jedna od karakteristika NRD-a je motiv poput leucinskog zatvarača. Tačkaste mutacije koje remete ovaj motiv također pojačavaju potencijal transaktivacije i transformacije MYB-a. Protein-1a koji veže mišji Myb (Mybbp1a), izvorno nazvan P160, identificiran je njegovom sposobnošću specifične interakcije s Myb, putem ovog motiva sličnog leucinskom zatvaraču. Zato je predloženo da Mybbp1a može modulirati MYB aktivnost nakon vezanja za MYB NRD. Mybbp1a je sveprisutno eksprimiran.[9][10]
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000132382 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000040463 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Keough R, Woollatt E, Crawford J, Sutherland GR, Plummer S, Casey G, Gonda TJ (Mar 2000). "Molecular cloning and chromosomal mapping of the human homologue of MYB binding protein (P160) 1A (MYBBP1A) to 17p13.3". Genomics. 62 (3): 483–9. doi:10.1006/geno.1999.6035. PMID 10644447.
- ^ "Entrez Gene: MYBBP1A MYB binding protein (P160) 1a".
- ^ "UniProt, Q9BQG0". Pristupljeno 12. 9. 2017.
- ^ Keough, R., Woollatt, E., Crawford, J., Sutherland, G. R., Plummer, S., Casey, G., Gonda, T. J. Molecular cloning and chromosomal mapping of the human homologue of MYB binding protein (P160) 1A (MYBBP1A) to 17p13.3. Genomics 62: 483-489, 1999. [PubMed]: 10644447
- ^ Favier, D., Gonda, T. J. Detection of proteins that bind to the leucine zipper motif of c-Myb. Oncogene 9: 305-311, 1994. [PubMed]: 8302594
- ^ Tavner, F. J., Simpson, R., Tashiro, S., Favier, D., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Macmillan, E. M., Lutwyche, J., Keough, R. A., Ishii, S., Gonda, T. J. Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role in transactivation by Myb. Molec. Cell Biol. 18: 989-1002, 1998. PubMed: 9447996
Dopunska literatura uredi
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- Fuller HR, Man NT, Lam LT, et al. (2010). "The SMN interactome includes Myb-binding protein 1a". J. Proteome Res. 9 (1): 556–63. doi:10.1021/pr900884g. PMID 19928837.