MTIF2

MTIF2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2CRV
Identifikatori
Aliasi	MTIF2
Vanjski ID-jevi	OMIM: 603766 MGI: 1924034 HomoloGene: 1840 GeneCards: MTIF2
Lokacija gena (čovjek)
Hrom.	Hromosom 2 (čovjek)
Kraj	55,269,347 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	29,495,279 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• nucleotide binding; • GTP binding; • translation factor activity, RNA binding; • translation initiation factor activity; • ribosomal small subunit binding; • vezivanje sa RNK; • GO:0006184 GTPase activity;
Ćelijska komponenta	• intracellular anatomical structure; • mitohondrija; • nukleoplazma;
Biološki proces	• translational initiation; • ribosome disassembly; • Biosinteza bjelančevina; • regulation of translational initiation; • mitochondrial translational initiation;
	Izvori:Amigo / QuickGO
Ortolozi
	4528
	76784
	ENSG00000085760
	ENSMUSG00000020459
	P46199; Q6P1N2
	Q91YJ5
NM_001005369; NM_002453; NM_001321001; NM_001321002; NM_001321003;
	NM_001321004; NM_001321005
	NM_001282118; NM_001282119; NM_001282120; NM_133767
NP_001005369; NP_001307930; NP_001307931; NP_001307932; NP_001307933;
	NP_001307934; NP_002444; NP_001005369.1; NP_002444.2
	NP_001269047; NP_001269048; NP_001269049; NP_598528
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Mitohondrijski faktor translacijske inicijacije IF-2 jest protein koji je kod ljudi kodiran genom MTIF2 sa hromosoma 2.^[5]^[6]^[7]

Amiokiselininska sekvenca

Dužina polipeptidnog lanca je 727 aminokiselina, a molkekulska iežina 81.317 Da.^[8]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MNQKLLKLEN	LLRFHTIYRQ	LHSLCQRRAL	RQWRHGFSSA	YPVWTAQLCA
WPWPTDVLTG	AALSQYRLLV	TKKEEGPWKS	QLSSTKSKKV	VEVWIGMTIE
ELARAMEKNT	DYVYEALLNT	DIDIDSLEAD	SHLDEVWIKE	VITKAGMKLK
WSKLKQDKVR	KNKDAVRRPQ	ADPALLTPRS	PVVTIMGHVD	HGKTTLLDKF
RKTQVAAVET	GGITQHIGAF	LVSLPSGEKI	TFLDTPGHAA	FSAMRARGAQ
VTDIVVLVVA	ADDGVMKQTV	ESIQHAKDAQ	VPIILAVNKC	DKAEADPEKV
KKELLAYDVV	CEDYGGDVQA	VPVSALTGDN	LMALAEATVA	LAEMLELKAD
PNGPVEGTVI	ESFTDKGRGL	VTTAIIQRGT	LRKGSVLVAG	KCWAKVRLMF
DENGKTIDEA	YPSMPVGITG	WRDLPSAGEE	ILEVESEPRA	REVVDWRKYE
QEQEKGQEDL	KIIEEKRKEH	KEAHQKAREK	YGHLLWKKRS	ILRFLERKEQ
IPLKPKEKRE	RDSNVLSVII	KGDVDGSVEA	ILNIIDTYDA	SHECELELVH
FGVGDVSAND	VNLAETFDGV	IYGFNVNAGN	VIQQSAAKKG	VKIKLHKIIY
RLVEDLQEEL	SSRLPCAVEE	HPVGEASILA	TFSVTEGKKK	VPVAGCRVQK
GQLEKQKKFK	LTRNGHVIWK	GSLTSLKHHK	DDISIVKTGM	DCGLSLDEDN
MEFQVGDRIV	CYEEKQIQAK	TSWDPGF

Funkcija

Tokom inicijacije biosinteze proteina, inicijacijski faktor-2 (IF-2) promovira vezivanje inicijatorske tRNK za malu podjedinicu ribosoma na način ovisan o GTP. Prokariotski IF-2 je jedan polipeptid, dok je eukariotski citoplazmatski IF-2 (eIF-2) trimerni protein. Mitohondrije goveđe jetre sadrže IF-2(mt), monomerni protein od 85 kD koji je ekvivalentan prokariotskom IF-2. Predviđeni ljudski protein od 727 aminokiselina sadrži sekvencu od 29 aminokiselina. Ljudski IF-2(mt) dijeli 32 do 38% identičnosti aminokiselinske sekvence sa IF-2(mt) kvasca i nekoliko prokariotskih IF-2, sa najvećim stepenom konzerviranosti u G-domenima proteina. Za ovaj gen su pronađene dvije varijante transkripta koje kodiraju isti protein.^[7]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000085760 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020459 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Bonner DS, Wiley JE, Farwell MA (Mar 1999). "Assignment1 of the mitochondrial translational initiation factor 2 gene (MTIF2) to human chromosome 2 bands p16→p14 by in situ hybridization and with somatic cell hybrids". Cytogenet Cell Genet. 83 (1–2): 80–1. doi:10.1159/000015133. PMID 9925935. S2CID 23290955.
^ Ma L, Spremulli LL (Feb 1995). "Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA". J Biol Chem. 270 (4): 1859–65. doi:10.1074/jbc.270.4.1859. PMID 7829522.
^ ^a ^b "Entrez Gene: MTIF2 mitochondrial translational initiation factor 2".
^ "UniProt, P46199" (jezik: engleski). Pristupljeno 13. 11. 2021.

Dopunska literatura

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Overman RG, Enderle PJ, Farrow JM, et al. (2003). "The human mitochondrial translation initiation factor 2 gene (MTIF2): transcriptional analysis and identification of a pseudogene". Biochim. Biophys. Acta. 1628 (3): 195–205. doi:10.1016/s0167-4781(03)00144-1. PMID 12932832.
Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
Miura E, Kato Y, Matsushima R, et al. (2007). "The Balance between Protein Synthesis and Degradation in Chloroplasts Determines Leaf Variegation in Arabidopsis yellow variegated Mutants". Plant Cell. 19 (4): 1313–28. doi:10.1105/tpc.106.049270. PMC 1913758. PMID 17416734.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000085760 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000020459 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9925935-5] Bonner DS, Wiley JE, Farwell MA (Mar 1999). "Assignment1 of the mitochondrial translational initiation factor 2 gene (MTIF2) to human chromosome 2 bands p16→p14 by in situ hybridization and with somatic cell hybrids". Cytogenet Cell Genet. 83 (1–2): 80–1. doi:10.1159/000015133. PMID 9925935. S2CID 23290955.

[pmid7829522-6] Ma L, Spremulli LL (Feb 1995). "Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA". J Biol Chem. 270 (4): 1859–65. doi:10.1074/jbc.270.4.1859. PMID 7829522.

[entrez-7] "Entrez Gene: MTIF2 mitochondrial translational initiation factor 2".

[UniProt-8] "UniProt, P46199" (jezik: engleski). Pristupljeno 13. 11. 2021.

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