FAM76B

FAM76B
Identifikatori
Aliasi	FAM76B
Vanjski ID-jevi	MGI: 1920076 HomoloGene: 25107 GeneCards: FAM76B
Lokacija gena (čovjek)
Hrom.	Hromosom 11 (čovjek)
Kraj	95,790,409 bp
Lokacija gena (miš)
Hrom.	Hromosom 9 (miš)
Kraj	13,766,283 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• jedro; • nuclear speck; • membrana; • integral component of membrane;
Biološki proces	• protein desumoylation;
	Izvori:Amigo / QuickGO
Ortolozi
	143684
	72826
	ENSG00000077458
	ENSMUSG00000037808
	Q5HYJ3
	Q80XP8
	NM_144664; NM_001330357
	NM_176836; NM_001358259
	NP_001317286; NP_653265
	NP_789806; NP_001345188
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Protein FAM76B je protein koji je kod ljudi kodiran genom FAM76B.^[5]^[6]

Ljudski FAM76B (hFAM76B) je protein od 39 kDa koji sadrži homopolimerne histidinske traktove, signal za ciljanje jedarnih pjega. FAM76B je visoko konzerviraan među različitim vrstama, što sugerira da može imati važnu fiziološku ulogu u normalnim ćelijskim funkcijama. Međutim, nedostatak odgovarajućih alata omeo je proučavanje ovog potencijalno važnog proteina. Kako bi se olakšalo istraživanje bioloških funkcija FAM76B, mišja monoklonska antitijela (MAbs) protiv hFAM76B su generirana korištenjem pročišćenog, prokariotski eksprimiranog proteina hFAM76B. Dobijeno je i karakterizirano šest sojeva MAb specifičnih za hFAM76B. Specifičnost MAb potvrđena je pomoću ćelijske linije FAM76B –/– HEK 293. Dvostruka imunofluorescencija praćena laserskom konfokusnom mikroskopijom potvrdila je jedarnu pjegavost, lokalizaciju hFAM76B, a specifična područja koja su prepoznali različiti MAb dodatno su razjašnjena Western blotom. Zbog visoke konzerviranosti proteinskih sekvenci između mišjeg i ljudsskog FAM76B, pokazano je da MAb protiv hFAM76B reagira specifično s mišjim FAM76B (mFAM76B). Konačno, otkriveno je da se FAM76B eksprimira u normalnim tkivima većine ljudskih organa, iako u različitoj mjeri. MAb proizvedeni u ovoj studiji trebali bi pružiti koristan alat za istraživanje bioloških funkcija FAM76B.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 339 aminokiselina, a molekulska težina 38.708 Da.^[7]

10	20	30	40	50
MAASALYACT	KCTQRYPFEE	LSQGQQLCKE	CRIAHPIVKC	TYCRSEFQQE
SKTNTICKKC	AQNVKQFGTP	KPCQYCNIIA	AFIGTKCQRC	TNSEKKYGPP
QTCEQCKQQC	AFDRKEEGRR	KVDGKLLCWL	CTLSYKRVLQ	KTKEQRKSLG
SSHSNSSSSS	LTEKDQHHPK	HHHHHHHHHH	RHSSSHHKIS	NLSPEEEQGL
WKQSHKSSAT	IQNETPKKKP	KLESKPSNGD	SSSINQSADS	GGTDNFVLIS
QLKEEVMSLK	RLLQQRDQTI	LEKDKKLTEL	KADFQYQESN	LRTKMNSMEK
AHKETVEQLQ	AKNRELLKQV	AALSKGKKFD	KSGSILTSP

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000077458 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000037808 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA (Dec 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc Natl Acad Sci U S A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
^ "Entrez Gene: FAM76B family with sequence similarity 76, member B".
^ "UniProt, Q5HYJ3". Pristupljeno 22. 8. 2021.

Dopunska literatura

Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Wiemann S, Weil B, Wellenreuther R (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Ota T, Suzuki Y, Nishikawa T (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Beausoleil SA, Jedrychowski M, Schwartz D (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Gerhard DS, Wagner L, Feingold EA (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wiemann S, Arlt D, Huber W (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Rual JF, Venkatesan K, Hao T (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Mehrle A, Rosenfelder H, Schupp I (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Olsen JV, Blagoev B, Gnad F (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
Vasilescu J, Zweitzig DR, Denis NJ (2007). "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells". J. Proteome Res. 6 (1): 298–305. CiteSeerX 10.1.1.401.4220. doi:10.1021/pr060438j. PMID 17203973.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000077458 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000037808 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12477932-5] Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA (Dec 2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc Natl Acad Sci U S A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.

[entrez-6] "Entrez Gene: FAM76B family with sequence similarity 76, member B".

[UniProt-7] "UniProt, Q5HYJ3". Pristupljeno 22. 8. 2021.

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