EPPK1
Epiplakin je protein koji je kod ljudi kodiran genom EPPK1.[4][5][6] Pripada porodici plakinskih proteina i nalazi se u ljudskoj epidermi.[4] Sastoji se od 13 domena, od kojih su svi slični domenu B koji se nalazi na C-kraju ljudske epiderme i srčanomišićnog proteina dezmoplakina.[4] Domeni u epiplakinu kreću se od 46-70% u homologiji ovog B domena u dezmoplakinu.[7] Prvi put je identificiran kao autoantigen kod osobe koja boluje od rijetke autoimunske kožne bolesti.[4] Epiplakin je sekvenciran tako da ima ukupno 5.065 aminokiselinskih ostataka i, na osnovu aminokiselinskog sastava, ima molekulsku masu od oko 552 kDa.[4] Gen EPPK1 je značajan po nedostatku introna u svom kodirajućem području - cijelo njegovo kodirajuće područje od 15 kbp kodirano je jednim exon.[8]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca je 5.090 aminokiselina, а molekulska težina 555.621 Da.[9]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MSGHTLPPLP | VPGTNSTEQA | SVPRAMAATL | GAGTPPRPQA | RSIAGVYVEA | ||||
| SGQAQSVYAA | MEQGLLPAGL | GQALLEAQAA | TGGLVDLARG | QLLPVSKALQ | ||||
| QGLVGLELKE | KLLAAERATT | GYPDPYGGEK | LALFQAIGKE | VVDRALGQSW | ||||
| LEVQLATGGL | VDPAQGVLVA | PEPACHQGLL | DRETWHKLSE | LEPGTGDLRF | ||||
| LNPNTLERLT | YHQLLERCVR | APGSGLALLP | LKITFRSMGG | AVSAAELLEV | ||||
| GILDEQAVQG | LREGRLAAVD | VSARAEVRRY | LEGTGSVAGV | VLLPEGHKKS | ||||
| FFQAATEHLL | PMGTALPLLE | AQAATHTLVD | PITGQRLWVD | EAVRAGLVSP | ||||
| ELHEQLLVAE | QAVTGHHDPF | SGSQIPLFQA | MKKGLVDRPL | ALRLLDAQLA | ||||
| TGGLVCPARR | LRLPLEAALR | CGCLDEDTQR | QLSQAGSFSD | GTHGGLRYEQ | ||||
| LLALCVTDPE | TGLAFLPLSG | GPRGGEPQGP | PFIKYSTRQA | LSTATATVSV | ||||
| GKFRGRPVSL | WELLFSEAIS | SEQRAMLAQQ | YQEGTLSVEK | LAAELSATLE | ||||
| QAAATARVTF | SGLRDTVTPG | ELLKAEIIDQ | DLYERLEHGQ | ATAKDVGSLA | ||||
| SAQRYLQGTG | CIAGLLLPGS | QERLSIYEAR | CKGLLRPGTA | LILLEAQAAT | ||||
| GFIIDPKANK | GHSVEEALRA | AVIGPDVFAK | LLSAERAVTG | YTDPYTGQQI | ||||
| SLFQAMQKGL | IVREHGIRLL | EAQIATGGVI | DPVHSHRVPV | DVAYRRGYFD | ||||
| QMLNLILLDP | SDDTKGFFDP | NTHENLTYLQ | LLERCVRDPE | TGLYLLPLSS | ||||
| TQSPLVDSAT | QQAFQNLLLS | VKYGRFQGQR | VSAWELINSE | YFSEGRRRQL | ||||
| LRRYRQREVT | LGQVAKLLEA | ETQRQADIML | PALRSRVTVH | QLLEAGIIDQ | ||||
| QLLDQVLAGT | ISPEALLLMD | GVRRYLCGLG | AVGGVRLLPS | GQRLSLYQAM | ||||
| RQKLLGPRVA | LALLEAQAAT | GTIMDPHSPE | SLSVDEAVRR | GVVGPELYGR | ||||
| LKRAEGAIAG | FRDPFSGKQV | SVFQAMKKGL | IPWEQAARLL | EAQVATGGII | ||||
| DPTSHHHLPM | PVAIQRGYVD | QEMETALSSS | SETFPTPDGQ | GRTSYAQLLE | ||||
| ECPRDETSGL | HLLPLPESAP | ALPTEEQVQR | SLQAVPGAKD | GTSLWDLLSS | ||||
| CHFTEEQRRG | LLEDVQEGRT | TVPQLLASVQ | RWVQETKLLA | QARVMVPGPR | ||||
| GEVPAVWLLD | AGIITQETLE | ALAQGTQSPA | QVAEQPAVKA | CLWGTGCVAG | ||||
| VLLQPSGAKA | SIAQAVRDGL | LPTGLGQRLL | EAQVASGFLV | DPLNNQRLSV | ||||
| EDAVKVGLVG | RELSEQLGQA | ERAAAGYPDP | YSRASLSLWQ | AMEKGLVPQN | ||||
| EGLPLLQVQL | ATGGVVDPVH | GVHLPQAAAC | RLGLLDTQTS | QVLTAVDKDN | ||||
| KFFFDPSARD | QVTYQQLRER | CVCDSETGLL | LLPLPSDTVL | EVDDHTAVAL | ||||
| RAMKVPVSTG | RFKGCSVSLW | DLLLSEYVGA | DKRRELVALC | RSGRAAALRQ | ||||
| VVSAVTALVE | AAERQPLQAT | FRGLRKQVSA | RDLFRAQLIS | RKTLDELSQG | ||||
| TTTVKEVAEM | DSVKRSLEGG | NFIAGVLIQG | TQERMSIPEA | LRRHILRPGT | ||||
| ALVLLEAQAA | TGFIIDPAEN | RKLTVEEAFK | AGMFGKETYV | KLLSAERAVT | ||||
| GYTDPYTGQQ | ISLFQAMQKD | LIVREHGIRL | LEAQIATGGI | IDPVHSHRVP | ||||
| VDVAYRCGYF | DEEMNRILAD | PSDDTKGFFD | PNTHENLTYL | QLLERCVEDP | ||||
| ETGLYLLQII | KKGENYVYIN | EATRHVLQSR | TAKMRVGRFA | DQVVSFWDLL | ||||
| SSPYFTEDRK | RELIQEYGAQ | SGGLEKLLEI | ITTTIEETET | QNQGIKVAAI | ||||
| RGEVTAADLF | NSRVIDQKTL | HTLRVGRTGG | QALSTLECVK | PYLEGSDCIA | ||||
| GVTVPSTREV | MSLHEASRKE | LIPAAFATWL | LEAQAATGFL | LDPCTRQKLS | ||||
| VDEAVDVGLV | NEELRERLLK | AERAATGYRD | PATGDTIPLF | QAMQKQLIEK | ||||
| AEALRLLEVQ | VATGGVIDPQ | HHHRLPLETA | YRRGCLHKDI | YALISDQKHM | ||||
| RKRFVDPNTQ | EKVSYRELQE | RCRPQEDTGW | VLFPVNKAAR | DSEHIDDETR | ||||
| RALEAEQVEI | TVGRFRGQKP | TLWALLNSEY | VTEEKKLQLV | RMYRTHTRRA | ||||
| LQTVAQLILE | LIEKQETSNK | HLWFQGIRRQ | ITASELLSSA | IITEEMLQDL | ||||
| ETGRSTTQEL | MEDDRVKRYL | EGTSCIAGVL | VPAKDQPGRQ | EKMSIYQAMW | ||||
| KGVLRPGTAL | VLLEAQAATG | FVIDPVRNLR | LSVEEPVPAG | VVGSEIQEKL | ||||
| LSAERAVTGY | TDPYTGQQIS | LFQAMQKDLI | VREHGIRLLE | AQIATGGVID | ||||
| PVHSHRVPVD | VAYRRGYFDE | EMNRVLADPS | DDTKGFFDPN | THENLTYVQL | ||||
| LRRCVPDPDT | GLYMLQLAGR | GSAVHQLSEE | LRCALRDARV | TPGSGALQGQ | ||||
| SVSVWELLFY | REVSEDRRQD | LLSRYRAGTL | TVEELGATLT | SLLAQAQAQA | ||||
| RAEAEAGSPR | PDPREALRAA | TMEVKVGRLR | GRAVPVWDVL | ASGYVSRAAR | ||||
| EELLAEFGSG | TLDLPALTRR | LTAIIEEAEE | APGARPQLQD | ARRGPREPGP | ||||
| AGRGDGDSGR | SQREGQGEGE | TQEAAAAAAA | ARRQEQTLRD | ATMEVQRGQF | ||||
| QGRPVSVWDV | LFSSYLSEAR | RDELLAQHAA | GALGLPDLVA | VLTRVIEETE | ||||
| ERLSKVSFRG | LRRQVSASEL | HTSGILGPET | LRDLAQGTKT | LQEVTEMDSV | ||||
| KRYLEGTSCI | AGVLVPAKDQ | PGRQEKMSIY | QAMWKGVLRP | GTALVLLEAQ | ||||
| AATGFVIDPV | RNLRLSVEEA | VAAGVVGGEI | QEKLLSAERA | VTGYTDPYTG | ||||
| QQISLFQAMQ | KDLIVREHGI | RLLEAQIATG | GVIDPVHSHR | VPVDVAYRRG | ||||
| YFDEEMNRVL | ADPSDDTKGF | FDPNTHENLT | YVQLLRRCVP | DPDTGLYMLQ | ||||
| LAGRGSAVHQ | LSEELRCALR | DARVTPGSGA | LQGQSVSVWE | LLFYREVSED | ||||
| RRQDLLSRYR | AGTLTVEELG | ATLTSLLAQA | QAQARAEAEA | GSPRPDPREA | ||||
| LRAATMEVKV | GRLRGRAVPV | WDVLASGYVS | GAAREELLAE | FGSGTLDLPA | ||||
| LTRRLTAIIE | EAEEAPGARP | QLQDAWRGPR | EPGPAGRGDG | DSGRSQREGQ | ||||
| GEGETQEAAA | AAAAARRQEQ | TLRDATMEVQ | RGQFQGRPVS | VWDVLFSSYL | ||||
| SEARRDELLA | QHAAGALGLP | DLVAVLTRVI | EETEERLSKV | SFRGLRRQVS | ||||
| ASELHTSGIL | GPETLRDLAQ | GTKTLQEVTE | MDSVKRYLEG | TSCIAGVLVP | ||||
| AKDQPGRQEK | MSIYQAMWKG | VLRPGTALVL | LEAQAATGFV | IDPVRNLRLS | ||||
| VEEAVAAGVV | GGEIQEKLLS | AERAVTGYTD | PYTGQQISLF | QAMQKDLIVR | ||||
| EHGIRLLEAQ | IATGGVIDPV | HSHRVPVDVA | YRRGYFDEEM | NRVLADPSDD | ||||
| TKGFFDPNTH | ENLTYVQLLR | RCVPDPDTGL | YMLQLAGRGS | AVHQLSEELR | ||||
| CALRDARVTP | GSGALQGQSV | SVWELLFYRE | VSEDRRQDLL | SRYRAGTLTV | ||||
| EELGATLTSL | LAQAQAQARA | EAEAGSPRPD | PREALRAATM | EVKVGRLRGR | ||||
| AVPVWDVLAS | GYVSGAAREE | LLAEFGSGTL | DLPALTRRLT | AIIEEAEEAP | ||||
| GARPQLQDAW | RGPREPGPAG | RGDGDSGRSQ | REGQGEGETQ | EAAAAAAAAR | ||||
| RQEQTLRDAT | MEVQRGQFQG | RPVSVWDVLF | SSYLSEARRD | ELLAQHAAGA | ||||
| LGLPDLVAVL | TRVIEETEER | LSKVSFRGLR | RQVSASELHT | SGILGPETLR | ||||
| DLAQGTKTLQ | EVTEMDSVKR | YLEGTSCIAG | VLVPAKDQPG | RQEKMSIYQA | ||||
| MWKGVLRPGT | ALVLLEAQAA | TGFVIDPVRN | LRLSVEEAVA | AGVVGGEIQE | ||||
| KLLSAERAVT | GYTDPYTGQQ | ISLFQAMQKD | LIVREHGIRL | LEAQIATGGV | ||||
| IDPVHSHRVP | VDVAYRRGYF | DEEMNRVLAD | PSDDTKGFFD | PNTHENLTYV | ||||
| QLLRRCVPDP | DTGLYMLQLA | GRGSAVHQLS | EELRCALRDA | RVTPGSGALQ | ||||
| GQSVSVWELL | FYREVSEDRR | QDLLSRYRAS | TLTVEELGAT | LTSLLAQAQA | ||||
| QARAEAEAGS | PRPDPREALR | AATMEVKVGR | LRGRAVPVWD | VLASGYVSRA | ||||
| AREELLAEFG | SGTLDLPALT | RRLTAIIEEA | EEAPGARPQL | QDAWRGPREP | ||||
| GPAGRGDGDS | GRSQREGQGE | GETQEAAAAT | AAARRQEQTL | RDATMEVQRG | ||||
| QFQGRPVSVW | DVLFSSYLSE | ARRDELLAQH | AAGALGLPDL | VAVLTRVIEE | ||||
| TEERLSKVSF | RGLRRQVSAS | ELHTSGILGP | ETLRDLAQGT | KTLQEVTEMD | ||||
| SVKRYLEGTS | CIAGVLVPAK | DQPGRQEKMS | IYQAMWKGVL | RPGTALVLLE | ||||
| AQAATGFVID | PVRNLRLSVE | EAVAAGVVGG | EIQEKLLSAE | RAVTGYTDPY | ||||
| TGQQISLFQA | MQKDLIVREH | GIRLLEAQIA | TGGVIDPVHS | HRVPVDVAYR | ||||
| RGYFDEEMNR | VLADPSDDTK | GFFDPNTHEN | LTYVQLLRRC | VPDPDTGLYM | ||||
| LQLAGRGSAV | HQLSEELRCA | LRDARVTPGS | GALQGQSVSV | WELLFYREVS | ||||
| EDRRQDLLSR | YRAGTLTVEE | LGATLTSLLA | QAQAQARAEA | EAGSPRPDPR | ||||
| EALRAATMEV | KVGRLRGRAV | PVWDVLASGY | VSGAAREELL | AEFGSGTLDL | ||||
| PALTRRLTAI | IEEAEEAPGA | RPQLQDAWRG | PREPGPAGRG | DGDSGRSQRE | ||||
| GQGEGETQEA | AAAAAAARRQ | EQTLRDATME | VQRGQFQGRP | VSVWDVLFSS | ||||
| YLSEARRDEL | LAQHAAGALG | LPDLVAVLTR | VIEETEERLS | KVSFRGLRRQ | ||||
| VSASELHTSG | ILGPETLRDL | AQGTKTLQEV | TEMDSVKRYL | EGTSCIAGVL | ||||
| VPAKDQPGRQ | EKMSIYQAMW | KGVLRPGTAL | VLLEAQAATG | FVIDPVRNLR | ||||
| LSVEEAVAAG | VVGGEIQEKL | LSAERAVTGY | TDPYTGQQIS | LFQAMQKDLI | ||||
| VREHGIRLLE | AQIATGGVID | PVHSHRVPVD | VAYRRGYFDE | EMNRVLADPS | ||||
| DDTKGFFDPN | THENLTYLQL | LQRATLDPET | GLLFLSLSLQ |
Funkcija
urediUtvrđeno je da se epiplakin veže za keratinske niti i može pridonijeti inhibiranju njihovog rasta. Sumnja se na to da se epiplakin vezan za keratin nalazi prvenstveno na granama i krajevima keratinskih niti.[10] Blokiranje ekspresije epiplakina u kultiviranim epitelnim ćelijama rožnjače putem siRNK povezano je s bržim zatvaranjem rana i bržom migracijom rožnjačnih ćelija.[11] To može biti posljedica gubitka epiplakina, što dovodi do modifikacije citoskeleta u epitelnim ćelijama.[11]
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000261150 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b c d e Fujiwara S, Takeo N, Otani Y, Parry DA, Kunimatsu M, Lu R, et al. (april 2001). "Epiplakin, a novel member of the Plakin family originally identified as a 450-kDa human epidermal autoantigen. Structure and tissue localization". The Journal of Biological Chemistry. 276 (16): 13340–7. doi:10.1074/jbc.M011386200. PMID 11278896.
- ^ Jang SI, Kalinin A, Takahashi K, Marekov LN, Steinert PM (februar 2005). "Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks". Journal of Cell Science. 118 (Pt 4): 781–93. doi:10.1242/jcs.01647. PMID 15671067.
- ^ "Entrez Gene: EPPK1 epiplakin 1".
- ^ Takeo N, Wang W, Matsuo N, Sumiyoshi H, Yoshioka H, Fujiwara S (novembar 2003). "Structure and heterogeneity of the human gene for epiplakin (EPPK1)". The Journal of Investigative Dermatology. 121 (5): 1224–6. doi:10.1046/j.1523-1747.2003.12550_5.x. PMID 14708632.
- ^ Stewart., Scherer (2008). A short guide to the human genome. Cold Spring Harbor Laboratory Press. ISBN 978-0-87969-791-4. OCLC 301791139.
- ^ "UniProt, P58107" (jezik: engleski). Pristupljeno 16. 9. 2021.
- ^ Wang W, Sumiyoshi H, Yoshioka H, Fujiwara S (august 2006). "Interactions between epiplakin and intermediate filaments". The Journal of Dermatology. 33 (8): 518–27. doi:10.1111/j.1346-8138.2006.00127.x. PMID 16923132. S2CID 25372989.
- ^ a b Kokado M, Okada Y, Miyamoto T, Yamanaka O, Saika S (maj 2016). "Effects of epiplakin-knockdown in cultured corneal epithelial cells". BMC Research Notes. 9 (1): 278. doi:10.1186/s13104-016-2082-7. PMC 4873999. PMID 27206504.
Dopunska literatura
uredi- Fujiwara S, Kohno K, Iwamatsu A, Naito I, Shinkai H (maj 1996). "Identification of a 450-kDa human epidermal autoantigen as a new member of the plectin family". The Journal of Investigative Dermatology. 106 (5): 1125–30. doi:10.1111/1523-1747.ep12340171. PMID 8618051.
- Blagoev B, Kratchmarova I, Ong SE, Nielsen M, Foster LJ, Mann M (mart 2003). "A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling". Nature Biotechnology. 21 (3): 315–8. doi:10.1038/nbt790. PMID 12577067. S2CID 26838266.
- Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J (maj 2003). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nature Biotechnology. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
- Spazierer D, Fuchs P, Pröll V, Janda L, Oehler S, Fischer I, et al. (august 2003). "Epiplakin gene analysis in mouse reveals a single exon encoding a 725-kDa protein with expression restricted to epithelial tissues". The Journal of Biological Chemistry. 278 (34): 31657–66. doi:10.1074/jbc.M303055200. PMID 12791695.
- Takeo N, Wang W, Matsuo N, Sumiyoshi H, Yoshioka H, Fujiwara S (novembar 2003). "Structure and heterogeneity of the human gene for epiplakin (EPPK1)". The Journal of Investigative Dermatology. 121 (5): 1224–6. doi:10.1046/j.1523-1747.2003.12550_5.x. PMID 14708632.
- Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". Journal of Proteome Research. 4 (4): 1339–46. doi:10.1021/pr050048h. PMID 16083285.
- Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (april 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (novembar 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
- Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.