Amiokiselininska sekvenca
uredi
Dužina polipeptidnog lanca je 371 aminokiselina , a molekulska težina 42.573 Da .[ 8]
10 20 30 40 50 MDARRVPQKD LRVKKNLKKF RYVKLISMET SSSSDDSCDS FASDNFANTR LQSVREGCRT RSQCRHSGPL RVAMKFPARS TRGATNKKAE SRQPSENSVT DSNSDSEDES GMNFLEKRAL NIKQNKAMLA KLMSELESFP GSFRGRHPLP GSDSQSRRPR RRTFPGVASR RNPERRARPL TRSRSRILGS LDALPMEEEE EEDKYMLVRK RKTVDGYMNE DDLPRSRRSR SSVTLPHIIR PVEEITEEEL ENVCSNSREK IYNRSLGSTC HQCRQKTIDT KTNCRNPDCW GVRGQFCGPC LRNRYGEEVR DALLDPNWHC PPCRGICNCS FCRQRDGRCA TGVLVYLAKY HGFGNVHAYL KSLKQEFEMQ A
Ovaj gen je identificiran kao c-Myc gen koji reagira i ponaša se kao direktan c-Myc ciljni gen. Utvrđeno je da prekomjerna ekspresija ovog gena pojačava transformaciju limfoblastoidnih ćelija i nadopunjuje transformacijski defektni Myc kutijski mutant II, sugerirajući njegovu uključenost u c-Myc posredovanu transformaciju ćelija. Prijavljene su dvije alternativno prerađene varijante transkripta koje kodiraju različite izoforme .[ 7]
^ a b c GRCh38: Ensembl release 89: ENSG00000144354 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000055612 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Prescott JE, Osthus RC, Lee LA, Lewis BC, Shim H, Barrett JF, Guo Q, Hawkins AL, Griffin CA, Dang CV (Dec 2001). "A novel c-Myc-responsive gene, JPO1, participates in neoplastic transformation" . J Biol Chem . 276 (51): 48276–84. doi :10.1074/jbc.M107357200 PMID 11598121 . ^ Walker MG (Aug 2002). "Drug target discovery by gene expression analysis: cell cycle genes". Curr Cancer Drug Targets . 1 (1): 73–83. doi :10.2174/1568009013334241 . PMID 12188893 . ^ a b "Entrez Gene: CDCA7 cell division cycle associated 7" .^ "UniProt, Q9BWT1" (jezik: engleski). Pristupljeno 16. 11. 2021 .
Dopunska literatura
uredi
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene . 138 (1–2): 171–4. doi :10.1016/0378-1119(94)90802-8 . PMID 8125298 . Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene . 200 (1–2): 149–56. doi :10.1016/S0378-1119(97)00411-3 . PMID 9373149 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 PMID 14702039 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4" . Nature . 434 (7034): 724–31. doi :10.1038/nature03466 PMID 15815621 . Osthus RC, Karim B, Prescott JE, et al. (2005). "The Myc target gene JPO1/CDCA7 is frequently overexpressed in human tumors and has limited transforming activity in vivo" . Cancer Res . 65 (13): 5620–7. doi :10.1158/0008-5472.CAN-05-0536 . PMC 1224734 PMID 15994934 . Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes" . Genome Res . 16 (1): 55–65. doi :10.1101/gr.4039406 . PMC 1356129 PMID 16344560 . Goto Y, Hayashi R, Muramatsu T, et al. (2006). "JPO1/CDCA7, a novel transcription factor E2F1-induced protein, possesses intrinsic transcriptional regulator activity". Biochim. Biophys. Acta . 1759 (1–2): 60–8. doi :10.1016/j.bbaexp.2006.02.004 . PMID 16580749 .
