ALKBH3

AlkB homolog 3 alfa-ketoglutarat dioksigenaze je protein koji je kod ljudi kodiran genom ALKBH3.

ALKBH3
Dostupne strukture PDB Pretraga ortologa: PDBe RCSB Spisak PDB ID kodova 2IUW
Identifikatori
Aliasi	ALKBH3
Vanjski ID-jevi	OMIM: 610603 MGI: 1916363 HomoloGene: 16393 GeneCards: ALKBH3
Lokacija gena (čovjek) Hrom. Hromosom 11 (čovjek) Bend 11p11.2 Početak 43,880,811 bp Kraj 43,920,274 bp
Lokacija gena (miš) Hrom. Hromosom 2 (miš) Bend 2|2 E1 Početak 93,810,895 bp Kraj 93,841,152 bp
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • L-ascorbic acid binding • dioxygenase activity • vezivanje iona metala • DNA-N1-methyladenine dioxygenase activity • oxidoreductase activity • ferrous iron binding • mRNA N1-methyladenosine dioxygenase activity • 2-oxoglutarate-dependent dioxygenase activity • cytosine C-5 DNA demethylase activity Ćelijska komponenta • jedro • mitohondrija • nukleoplazma • citoplazma • citosol Biološki proces • Ćelijska proliferacija • cellular response to DNA damage stimulus • DNA dealkylation involved in DNA repair • oxidative single-stranded DNA demethylation • oxidative single-stranded RNA demethylation • GO:0100026 Popravka DNK Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	221120	69113
Ensembl	ENSG00000166199	ENSMUSG00000040174
UniProt	Q96Q83	Q8K1E6
RefSeq (mRNK)	NM_139178	NM_026944
RefSeq (bjelančevina)	NP_631917	NP_081220
Lokacija (UCSC)	Chr 11: 43.88 – 43.92 Mb	Chr 2: 93.81 – 93.84 Mb
PubMed pretraga
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 286 aminokiselina, a molekulska težina 33.375 Da.

10		20		30		40		50
MEEKRRRARV		QGAWAAPVKS		QAIAQPATTA		KSHLHQKPGQ		TWKNKEHHLS
DREFVFKEPQ		QVVRRAPEPR		VIDREGVYEI		SLSPTGVSRV		CLYPGFVDVK
EADWILEQLC		QDVPWKQRTG		IREDITYQQP		RLTAWYGELP		YTYSRITMEP
NPHWHPVLRT		LKNRIEENTG		HTFNSLLCNL		YRNEKDSVDW		HSDDEPSLGR
CPIIASLSFG		ATRTFEMRKK		PPPEENGDYT		YVERVKIPLD		HGTLLIMEGA
TQADWQHRVP		KEYHSREPRV		NLTFRTVYPD		PRGAPW

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

Funkcija

AlkB protein Escherichia coli štiti od citotoksičnosti metilacijskih agenasa, popravljanjem specifičnih molekulskih lezija DNK, nastalih u jednolančanoj DNK. ALKBH2 (MIM 610602) i ALKBH3 su homolozi AlkB-a 'E. coli koji kataliziraju uklanjanje 1-metiladenina i 3-metilcitozina (Duncan et al., 2002 PubMed 12486230]).

Dopunska literatura

• Sundheim O, Vågbø CB, Bjørås M, Sousa MM, Talstad V, Aas PA, Drabløs F, Krokan HE, Tainer JA, Slupphaug G (2006). "Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage". EMBO J. 25 (14): 3389–97. doi:10.1038/sj.emboj.7601219. PMC 1523172. PMID 16858410.
• Liu BQ, Wu YD, Li PH, Wei JX, Zhang T, Liu RL (2007). "Prostate cancer antigen-1 as a potential novel marker for prostate cancer". Asian J. Androl. 9 (6): 821–6. doi:10.1111/j.1745-7262.2007.00279.x. PMID 17968469.
• Monsen VT, Sundheim O, Aas PA, Westbye MP, Sousa MM, Slupphaug G, Krokan HE (2010). "Divergent ß-hairpins determine double-strand versus single-strand substrate recognition of human AlkB-homologues 2 and 3". Nucleic Acids Res. 38 (19): 6447–55. doi:10.1093/nar/gkq518. PMC 2965238. PMID 20525795.
• Chen B, Liu H, Sun X, Yang CG (2010). "Mechanistic insight into the recognition of single-stranded and double-stranded DNA substrates by ABH2 and ABH3". Mol Biosyst. 6 (11): 2143–9. doi:10.1039/c005148a. PMID 20714506.
• Malki K, Uher R, Paya-Cano J, Binder E, Rietschel M, Zobel A, Mors O, Hauser J, Henigsberg N, Jerman B, Souery D, Placentino A, Ng MY, Cohen-Woods S, Sluyter F, Farmer A, Aitchison KJ, Craig IW, Lewis CM, McGuffin P, Schalkwyk LC (2011). "Convergent animal and human evidence suggests a role of PPM1A gene in response to antidepressants". Biol. Psychiatry. 69 (4): 360–5. doi:10.1016/j.biopsych.2010.08.011. PMID 20970119. S2CID 41571515.
• Tasaki M, Shimada K, Kimura H, Tsujikawa K, Konishi N (2011). "ALKBH3, a human AlkB homologue, contributes to cell survival in human non-small-cell lung cancer". Br. J. Cancer. 104 (4): 700–6. doi:10.1038/sj.bjc.6606012. PMC 3049579. PMID 21285982.
• Dango S, Mosammaparast N, Sowa ME, Xiong LJ, Wu F, Park K, Rubin M, Gygi S, Harper JW, Shi Y (2011). "DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation". Mol. Cell. 44 (3): 373–84. doi:10.1016/j.molcel.2011.08.039. PMC 3258846. PMID 22055184.
• Koike K, Ueda Y, Hase H, Kitae K, Fusamae Y, Masai S, Inagaki T, Saigo Y, Hirasawa S, Nakajima K, Ohshio I, Makino Y, Konishi N, Yamamoto H, Tsujikawa K (2012). "anti-tumor effect of AlkB homolog 3 knockdown in hormone- independent prostate cancer cells". Curr Cancer Drug Targets. 12 (7): 847–56. doi:10.2174/156800912802429283. PMID 22515525.
• Yamato I, Sho M, Shimada K, Hotta K, Ueda Y, Yasuda S, Shigi N, Konishi N, Tsujikawa K, Nakajima Y (2012). "PCA-1/ALKBH3 contributes to pancreatic cancer by supporting apoptotic resistance and angiogenesis". Cancer Res. 72 (18): 4829–39. doi:10.1158/0008-5472.CAN-12-0328. PMID 22826605.