NAPG
Gama-rastvorljivi NSF pridodati protein je protein koji je kod ljudi kodiran genom NAPG.[5][6]
Aminokiselinska sekvenca
urediDužina polipeptidnog lanca mu je 312 aminokiselina, а molekulska masa — 34.746[7].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAAQKINEGL | EHLAKAEKYL | KTGFLKWKPD | YDSAASEYGK | AAVAFKNAKQ | ||||
| FEQAKDACLR | EAVAHENNRA | LFHAAKAYEQ | AGMMLKEMQK | LPEAVQLIEK | ||||
| ASMMYLENGT | PDTAAMALER | AGKLIENVDP | EKAVQLYQQT | ANVFENEERL | ||||
| RQAVELLGKA | SRLLVRGRRF | DEAALSIQKE | KNIYKEIENY | PTCYKKTIAQ | ||||
| VLVHLHRNDY | VAAERCVRES | YSIPGFNGSE | DCAALEQLLE | GYDQQDQDQV | ||||
| SDVCNSPLFK | YMDNDYAKLG | LSLVVPGGGI | KKKSPATPQA | KPDGVTATAA | ||||
| DEEEDEYSGG | LC |
Funkcija
urediNSF i SNAP (proteini za vezanje NSF) opći su elementi aparata ćelijskog membranskog transporta. Sekvenca predviđenih humanih proteina od 312 aminokiselina kodiranih NAPG-om je 95% identična sekvenci goveđeg gama-SNAP-a. NAPG u ovom procesu posreduje trombocitnu egzocitozu i kontrolira membransku fuziju.[6]
Reference
uredi- ^ a b c GRCh38: Ensembl release 89: ENSG00000134265 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024581 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Lemons PP, Chen D, Bernstein AM, Bennett MK, Whiteheart SW (Sep 1997). "Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery". Blood. 90 (4): 1490–500. doi:10.1182/blood.V90.4.1490. PMID 9269766.
- ^ a b "Entrez Gene: NAPG N-ethylmaleimide-sensitive factor attachment protein, gamma".
- ^ "UniProt, Q99747". Pristupljeno 12. 9. 2017.
Dopunska literatura
uredi- Wilson DW, Whiteheart SW, Wiedmann M, et al. (1992). "A multisubunit particle implicated in membrane fusion". J. Cell Biol. 117 (3): 531–8. doi:10.1083/jcb.117.3.531. PMC 2289450. PMID 1315316.
- Whiteheart SW, Brunner M, Wilson DW, et al. (1992). "Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes". J. Biol. Chem. 267 (17): 12239–43. PMID 1601890.
- Whiteheart SW, Griff IC, Brunner M, et al. (1993). "SNAP family of NSF attachment proteins includes a brain-specific isoform". Nature. 362 (6418): 353–5. Bibcode:1993Natur.362..353W. doi:10.1038/362353a0. PMID 8455721. S2CID 4341471.
- Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
- Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
- Mastick CC, Falick AL (1997). "Association of N-ethylmaleimide sensitive fusion (NSF) protein and soluble NSF attachment proteins-alpha and -gamma with glucose transporter-4-containing vesicles in primary rat adipocytes". Endocrinology. 138 (6): 2391–7. doi:10.1210/en.138.6.2391. PMID 9165027.
- Subramaniam VN, Loh E, Horstmann H, et al. (2000). "Preferential association of syntaxin 8 with the early endosome". J. Cell Sci. 113 (6): 997–1008. PMID 10683148.
- Chen D, Xu W, He P, et al. (2001). "Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria". J. Biol. Chem. 276 (16): 13127–35. doi:10.1074/jbc.M009424200. PMID 11278501.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Tani K, Shibata M, Kawase K, et al. (2003). "Mapping of functional domains of gamma-SNAP". J. Biol. Chem. 278 (15): 13531–8. doi:10.1074/jbc.M213205200. PMID 12554740.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.