Test vruće ploče

Test vruće ploče je test odgovora na bol kod životinja, sličan testu trzanja repa. Metodi vruće ploče i trzanja repa općenito se koriste za testiranje analgetika s centralnim djelovanjem,^[1]

Test vruće ploče koristi se u istraživanjima osnovnih bolova i u testiranju efikasnosti analgetika, promatranjem reakcije na bol uzrokovane toplinom. Predložili su ga Eddy i Leimbach 1953.^[2] Koristili su model ponašanja nocicepcije, gdje se ponašanja poput skakanja i lizanja stražnjih šapa izazivaju nakon štetnog toplinskog podražaja. Lizanje je brzi odgovor na bolne toplinske podražaje koji je direktan pokazatelj nocicepcijskog praga. Skakanje predstavlja razrađeniji odgovor, sa kašnjenjem i uključuje emocijsku komponentu bijega.^[3]

Postupak

Za držanje životinje na zagrijanoj površini ploče, upotrebljava se prozirni stakleni cilindar.^[4]
Temperatura ploče podešava se pomoću termoregulirane pumpe sa cirkulacijom vode.
Vrijeme latencije definira se kao vremenski period između nulte tačke, kada se životinja stavi na površinu ploče za grijanje, i vrijeme kada životinja liže šapu ili skoči kako bi izbjegla toplinsku bol. ^[5]^[6]

Reference

^ Carlsson, Karl-Heinz; Jurna, Ilmar (1987). "Depression by flupirtine, a novel analgesic agent, of motor and sensory responses of the nociceptive system in the rat spinal cord". European Journal of Pharmacology. 143 (1): 89–99. doi:10.1016/0014-2999(87)90738-2. ISSN 0014-2999. PMID 3691652.
^ Eddy, NB; Leimbach, D (1953). "Synthetic analgesics. II. Dithienylbutenyl- and dithienylbutylamines". J Pharmacol Exp Ther. 107 (3): 385–393. PMID 13035677.
^ ESPEJO, E; Mir, D (30 September 1993). "Structure of the rat's behaviour in the hot plate test". Behavioural Brain Research. 56 (2): 171–176. doi:10.1016/0166-4328(93)90035-O. PMID 8240711.
^ Hunskaar, Steinar; Berge, Odd-Geir; Hole, Kjell (1 August 1986). "A modified hot-plate test sensitivie to mild analgesics". Behavioural Brain Research. 21 (2): 101–108. doi:10.1016/0166-4328(86)90088-4. PMID 3755945.
^ Ripoll N, Hascoet M, Bourin M. The four-plates test: Anxiolytic or analgesic paradigm? Progress in Neuro-Psychopharmacology and Biological Psychiatry, July 2006 vol. 30 issue 5, 873-880.
^ Tzschentke, T. M.; Christoph, T.; Kogel, B.; Schiene, K.; Hennies, H.-H.; Englberger, W.; Haurand, M.; Jahnel, U.; Cremers, T. I. F. H.; Friderichs, E.; De Vry, J. (23 July 2007). "( )-(1R,2R)-3-(3-Dimethylamino-1-ethyl-2-methyl-propyl)-phenol Hydrochloride (Tapentadol HCl): a Novel -Opioid Receptor Agonist/Norepinephrine Reuptake Inhibitor with Broad-Spectrum Analgesic Properties". Journal of Pharmacology and Experimental Therapeutics. 323 (1): 265–276. doi:10.1124/jpet.107.126052. PMID 17656655.

Vanjski linkovi

[CarlssonJurna1987-1] Carlsson, Karl-Heinz; Jurna, Ilmar (1987). "Depression by flupirtine, a novel analgesic agent, of motor and sensory responses of the nociceptive system in the rat spinal cord". European Journal of Pharmacology. 143 (1): 89–99. doi:10.1016/0014-2999(87)90738-2. ISSN 0014-2999. PMID 3691652.

[orig-2] Eddy, NB; Leimbach, D (1953). "Synthetic analgesics. II. Dithienylbutenyl- and dithienylbutylamines". J Pharmacol Exp Ther. 107 (3): 385–393. PMID 13035677.

[3] ESPEJO, E; Mir, D (30 September 1993). "Structure of the rat's behaviour in the hot plate test". Behavioural Brain Research. 56 (2): 171–176. doi:10.1016/0166-4328(93)90035-O. PMID 8240711.

[4] Hunskaar, Steinar; Berge, Odd-Geir; Hole, Kjell (1 August 1986). "A modified hot-plate test sensitivie to mild analgesics". Behavioural Brain Research. 21 (2): 101–108. doi:10.1016/0166-4328(86)90088-4. PMID 3755945.

[5] Ripoll N, Hascoet M, Bourin M. The four-plates test: Anxiolytic or analgesic paradigm? Progress in Neuro-Psychopharmacology and Biological Psychiatry, July 2006 vol. 30 issue 5, 873-880.

[6] Tzschentke, T. M.; Christoph, T.; Kogel, B.; Schiene, K.; Hennies, H.-H.; Englberger, W.; Haurand, M.; Jahnel, U.; Cremers, T. I. F. H.; Friderichs, E.; De Vry, J. (23 July 2007). "( )-(1R,2R)-3-(3-Dimethylamino-1-ethyl-2-methyl-propyl)-phenol Hydrochloride (Tapentadol HCl): a Novel -Opioid Receptor Agonist/Norepinephrine Reuptake Inhibitor with Broad-Spectrum Analgesic Properties". Journal of Pharmacology and Experimental Therapeutics. 323 (1): 265–276. doi:10.1124/jpet.107.126052. PMID 17656655.

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