SERINC3
Serinski inkorporator 3 jest protein koji je kod ljudi kodiran genom SERINC3.[5][6] Demonstrirano je da se SERINC3 ponaša kao faktor restrikcije retrovirusa.[7][8][9]
Model organizmi uredi
U proučavanju funkcije SERINC3 upotrebljavani su u modwelni organizmi. Uvjetna linijas nokaut miša zvana Serinc3tm1a(KOMP)Wtsi generirana je u Wellcome Trust Sanger Institute.[10] Muške i ženske životinje prolazile su standardizirani fenotipski skrining[11] za određivanje efekata delecije.[12][13][14][15] Izvršeni dodatni snimcii: - Dubinsko imunsko fenotipiziranje[16]
| Svojstvo | Fenotip |
|---|---|
| Svi podaci raspoloživi su na linku.[11][16] | |
| Insulin | Normalan |
| Vijabilnost homozigota na P14 | Normalna |
| Plodnost homozigota | Normalna |
| Tjelesna težina | Normalna |
| Neurološka procjena | Normalna |
| Snaga stiska | Normalna |
| Dismorfologija | Normalna |
| Indirektana kalorimetrija | Normalna |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog debla | Normalan |
| DEXA | Normalna |
| Radiografija | Normalna |
| Morfologija oka | Normalna |
| Klinička hemija | Normalna |
| Hematologija 16 sedmica | Normal |
| Leukociti periferne krvi 16 sedmica | Normalni |
| Težina srca | Normalna |
| Salmonella infekcija | Normalna |
| Imunofenotipizacija slezene | Normalna |
| Imunofenotipizacija mezenternih limfnih čvorova | Normalna |
| Epidermni imunski sastav | Normalan |
| Izazov gripe | Normalan |
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000132824 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000017707 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Bossolasco M, Lebel M, Lemieux N, Mes-Masson AM (novembar 1999). "The human TDE gene homologue: localization to 20q13.1-13.3 and variable expression in human tumor cell lines and tissue". Molecular Carcinogenesis. 26 (3): 189–200. doi:10.1002/(SICI)1098-2744(199911)26:3<189::AID-MC8>3.0.CO;2-T. PMID 10559794.
- ^ "Entrez Gene: SERINC3 serine incorporator 3".
- ^ Rosa A, Chande A, Ziglio S, De Sanctis V, Bertorelli R, Goh SL, et al. (oktobar 2015). "HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation". Nature. 526 (7572): 212–7. doi:10.1038/nature15399. PMC 4861059. PMID 26416734.
- ^ Chande A, Cuccurullo EC, Rosa A, Ziglio S, Carpenter S, Pizzato M (novembar 2016). "S2 from equine infectious anemia virus is an infectivity factor which counteracts the retroviral inhibitors SERINC5 and SERINC3". Proceedings of the National Academy of Sciences of the United States of America. 113 (46): 13197–13202. doi:10.1073/pnas.1612044113. PMC 5135340. PMID 27803322.
- ^ Ramdas, Pavitra; Bhardwaj, Vipin; Singh, Aman; Vijay, Nagarjun; Chande, Ajit (24. 2. 2020). "Coevolution of retroviruses with SERINCs following whole-genome duplication divergence". bioRxiv. doi:10.1101/2020.02.24.962506.
- ^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- ^ a b "International Mouse Phenotyping Consortium".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, et al. (juni 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (juni 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (januar 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- ^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, et al. (juli 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- ^ a b "Infection and Immunity Immunophenotyping (3i) Consortium".[mrtav link]
Dopunska literatura uredi
- Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, et al. (septembar 1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). Nature. 377 (6547 Suppl): 3–174. PMID 7566098.
- Maruyama K, Sugano S (januar 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Bonaldo MF, Lennon G, Soares MB (septembar 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (oktobar 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Bossolasco M, Veillette F, Bertrand R, Mes-Masson AM (august 2006). "Human TDE1, a TDE1/TMS family member, inhibits apoptosis in vitro and stimulates in vivo tumorigenesis". Oncogene. 25 (33): 4549–58. doi:10.1038/sj.onc.1209488. PMID 16547497.